Interspecific Hybridization in Cucumis—Progress, Problems, and Perspectives

Chen, Jinfeng; Adelberg, Jeffrey

doi:10.21273/hortsci.35.1.11

Cited by 27 publications

(11 citation statements)

References 23 publications

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“…Rehm] (Wehenr and St. Amand 1993), while PIs of C. metuliferus and C. anguria were resistant to cucurbit yellowing stunting disorder virus (CYSDV) transmitted by whiteflies (B. tabaci) (Lopez-Sese and Gomez-Guillamon 2000). Genetic distances between C. anguria (PI 542135) and C. africanus (PI 542127) (35% genetic similarity) and between C. meeusei (PI 376068) and C. zeyheri (PI 532629) (61% genetic similarity) (Figure 2) are in agreement with crossability results among these species as summarized by Chen and Adelberg (2000).…”

Section: Genetic Diversity Among Citrullis and Cucumis Speciessupporting

confidence: 80%

Analysis based on RAPD and ISSR Markers Reveals Closer Similarities among Citrullus and Cucumis Species than with Praecitrullus fistulosus (Stocks) Pangalo

Levi

Thomas

Simmons

et al. 2005

Genet Resour Crop Evol

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A cucurbit species named Praecitrullus fistulosus (Stocks) Pangalo, which thrives in India, is considered to be a distant relative of watermelon. Recent experiments indicated that it has mild resistance to whiteflies (Bemisia tabaci). However, our attempts to cross various US plant introductions (PIs) of P. fistulosus with watermelon or other Citrullus PIs have not been successful. Thus, to determine genetic relatedness among those species, phylogenetic analysis [based on simple sequence repeat (SSR)-anchored (also termed ISSR), and randomly amplified polymorphic DNA (RAPD) markers] was conducted among PIs of P. fistulosus, Citrullus lanatus var. lanatus (watermelon), C. lanatus var. citroides and the wild Citrullus colocynthis. Phylogenetic relationships were also examined with Cucumis melo (melon), Cucumis sativus (cucumber), and wild Cucumis species including C. africanus, C. metuliferus, C. anguria, C. meeusei, and C. zeyheri. Wide genetic distance exists between Citrullus and Cucumis groups (8% genetic similarity). Phylogenetic relationships among Citrullus species and subspecies are closer (25-55% genetic similarity) as compared with those among most Cucumis species (14-68% genetic similarity). P. fistulosus appeared to be distant from both Cucumis and Citrullus species (genetic similarity between P. fistulosus and Cucumis or Citrullus groups is less than 3%). Although wide genetic differences and reproductive barriers exist among cucurbit species examined in this study, they are still considered as potential germplasm source for enhancing watermelon and melon crops using traditional breeding and biotechnology procedures.

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Section: Genetic Diversity Among Citrullis and Cucumis Speciessupporting

confidence: 80%

Analysis based on RAPD and ISSR Markers Reveals Closer Similarities among Citrullus and Cucumis Species than with Praecitrullus fistulosus (Stocks) Pangalo

Levi

Thomas

Simmons

et al. 2005

Genet Resour Crop Evol

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“…Allopolyploids can form through various processes, usually via interspecific hybridization and subsequent genome duplication. In addition, in light of the challenge of global diseases and climate change, ‘base broadening’ and the introgression of targeted genes through wide hybridization of domesticated crops and their wild relatives are more desirable than ever for breeders (Chen and Adelberg, ; Kumari et al ., ). In interspecific crosses, hybrids may show favoured growth vigour (heterosis); however, hybrids may also suffer from hybrid incompatibility, which inhibits allopolyploid speciation.…”

Section: Introductionmentioning

confidence: 99%

Allopolyploidization in Cucumis contributes to delayed leaf maturation with repression of redundant homoeologous genes

Wang

Hyldgaard

et al. 2018

The Plant Journal

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The important role of polyploidy in plant evolution is widely recognized. However, many questions remain to be explored to address how polyploidy affects the phenotype of the plant. To shed light on the phenotypic and molecular impacts of allopolyploidy, we investigated the leaf development of a synthesized allotetraploid (Cucumis × hytivus), with an emphasis on chlorophyll development. Delayed leaf maturation was identified in C. × hytivus, based on delayed leaf expansion, initial chlorophyll deficiency in the leaves and disordered sink-source transition. Anatomical observations also revealed disturbed chloroplast development in C. ×hytivus. The determination of chlorophyll biosynthesis intermediates suggested that the chlorophyll biosynthesis pathway of C. × hytivus is blocked at the site at which uroporphyrinogen III is catalysed to coproporphyrinogen III. Three chlorophyll biosynthesis-related genes, HEMA1, HEME2 and POR, were significantly repressed in C. × hytivus. Sequence alignment showed both synonymous and non-synonymous substitutions in the HEMA1, HEME2 and POR genes of the parents. Cloning of the chlorophyll biosynthetic genes suggested the retention of homoeologs. In addition, a chimeric clone of the HEMA1 gene that consisted of homologous genes from the parents was identified in C. × hytivus. Overall, our results showed that allopolyploidization in Cucumis has resulted in disturbed chloroplast development and reduced chlorophyll biosynthesis caused by the repressed expression of duplicated homologous genes, which further led to delayed leaf maturation in the allotetraploid, C. × hytivus. The preferential retention/loss of certain types of genes and non-reciprocal homoeologous recombination were also supported in the present study, which provides new insights into the impact of allopolyploidy.

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“…Moreover, C. metuliferus was reportedly able to provide competence for heat and salt tolerance (Benzioni et al, 1991), prolong fruit shelf life (Mendlinger et al, 1992), and increase resistance against Cucurbit yellowing stunting disorder virus (Lopez-Sese and Gomez-Guillamon, 2000), PRSV-P, PRSV-W (formerly known as WMV-1) and Squash mosaic virus (Provvidenti and Gonsalves, 1982;Robinson, 1974, 1977), whitefly (Lopez-Sese and Gomez-Guillamon, 2000), fusarium wilt caused by Fusarium oxysporum f. sp. melonis (Nisini et al, 2002), and root-knot nematode (Chen and Adelberg, 2000;Nugent and Dukes, 1997;Siguenza et al, 2005;Wehner et al, 1991).…”

mentioning

confidence: 99%

In Vitro Regeneration and Genetic Transformation of Cucumis metuliferus through Cotyledon Organogenesis

Lin¹,

Lin²,

Chung³

et al. 2011

horts

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This study was undertaken to establish for the first time an efficient regeneration and transformation system for Cucumis metuliferus line PI292190, which is the source of a well-defined resistant gene, Wmv, that provides resistance against Papaya ringspot virus type P (PRSV-P) and PRSV-W (formerly known as Watermelon mosaic virus 1, WMV-1). Different combinations of growth regulators were evaluated for the regeneration of cotyledon explants. Adventitious buds or shoot primordia were obtained within 3 to 4 weeks on regeneration medium. After shoot development, adventitious buds or shoot primordia were transferred to elongation medium for 3 to 4 weeks and these shoots were subcultured onto rooting medium for another 1 to 2 weeks. Under optimal culture conditions, a total of 7 to 10 weeks was necessary to obtain C. metuliferus plantlets from cotyledons. Furthermore, transgenic plants were successfully obtained using an Agrobacterium tumefaciens-mediated transformation method as shown by polymerase chain reaction analysis and histochemical β-glucuronidase (GUS) assay. A total of nine transgenic plants were developed from 360 cotyledon explants, giving a transformation frequency of 2.5%.

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Interspecific Hybridization in Cucumis—Progress, Problems, and Perspectives

Cited by 27 publications

References 23 publications

Analysis based on RAPD and ISSR Markers Reveals Closer Similarities among Citrullus and Cucumis Species than with Praecitrullus fistulosus (Stocks) Pangalo

Analysis based on RAPD and ISSR Markers Reveals Closer Similarities among Citrullus and Cucumis Species than with Praecitrullus fistulosus (Stocks) Pangalo

Allopolyploidization in Cucumis contributes to delayed leaf maturation with repression of redundant homoeologous genes

In Vitro Regeneration and Genetic Transformation of Cucumis metuliferus through Cotyledon Organogenesis

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