Interspecific potato somatic hybrids between Solanum tuberosum and S. cardiophyllum, potential sources of late blight resistance breeding

Chandel, Poonam; Tiwari, Jagesh Kumar; Ali, Nilofer; Devi, Sapna; Sharma, Sandeep; Sharma, Sanjeev; Luthra, S. K.; Singh, B. P.

doi:10.1007/s11240-015-0862-8

Cited by 38 publications

(15 citation statements)

References 35 publications

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“…Recently, after using biotechnological improvement Simplot Plant Sciences has reported ‘Innate’ Gen 2 potatoes that are of high quality and show a strong resistance to late blight in the second generation 1 . Consequently, the use of biotechnology and MAS will increase the speed with which other genes conferring resistance can be incorporated into potato cultivars and their subsequent cultivation ( Rietman, 2011 ; Chandel et al, 2015 ). Extensive investigations indicate that stacking of multiple R genes and monitoring how to deploy these stacks spatially and temporally could reduce fungicide use by over 80% ( Haverkort et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

et al. 2020

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Potato Late Blight Resistance Genes of the parent cultivar 'Delikat' in field trials. The evaluation of agronomic traits of selected BC 2 clones and of their processing qualities revealed valuable material for breeding late blight durable resistant potato. We show that the combination of somatic hybridization with the additional use of gene specific markers and corresponding Avr effectors is an efficient approach for the successful identification and introgression of late blight resistance genes into the potato gene pool.

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Section: Discussionmentioning

confidence: 99%

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

et al. 2020

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“…In heterologous hexaploid wheat, there are one or two amplicons in a single individual at an SSR locus (Yang, Peng & Yang, 2016; Sipahi et al, 2017). The autotetraploid plants alfalfa (Liu, Liu & Yang, 2007) and potato (Chandel et al, 2015) show one, two, three or four amplicons in single individuals at an SSR locus. We found that the number of bands in sainfoin individuals ranges from one amplicon to four amplicons.…”

Section: Discussionmentioning

confidence: 99%

Development of polymorphic EST-SSR markers and characterization of the autotetraploid genome of sainfoin (Onobrychis viciifolia)

Shen

Chai

Zhou

et al. 2019

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Background Sainfoin (Onobrychis viciifolia) is a highly nutritious, tannin-containing, and tetraploid forage legume. Due to the lack of detailed transcriptomic and genomic information on this species, genetic and breeding projects for sainfoin improvement have been significantly hindered. Methods In this study, a total of 24,630,711 clean reads were generated from 14 different sainfoin tissues using Illumina paired-end sequencing technology and deposited in the NCBI SRA database (SRX3763386). From these clean reads, 77,764 unigene sequences were obtained and 6,752 EST-SSRs were identified using de novo assembly. A total of 2,469 primer pairs were designed, and 200 primer pairs were randomly selected to analyze the polymorphism in five sainfoin wild accessions. Results Further analysis of 40 sainfoin individuals from the five wild populations using 61 EST-SSR loci showed that the number of alleles per locus ranged from 4 to 15, and the expected heterozygosity varied from 0.55 to 0.91. Additionally, by counting the EST-SSR band number and sequencing the three or four bands in one sainfoin individual, sainfoin was confirmed to be autotetraploid. This finding provides a high level of information about this plant. Discussion Through this study, 61 EST-SSR markers were successfully developed and shown to be useful for genetic studies and investigations of population genetic structures and variabilities among different sainfoin accessions.

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“…In specific cases, transfer of desirable resistance genes is possible only through methods different than sexual hybridization due to crossing barriers. Somatic hybrids resistant to P. infestans were previously obtained between Solanum tuberosum ( tbr) and several wild potato species (Orczyk et al 2003; Smyda et al 2013; Chandel et al 2015). Of this group, only few hybrids between tbr and Solanum bulbocastanum (Helgeson et al 1998), Solanum nigrum (Horsman et al 2001), Solanum tarnii (Thieme et al 2008), Solanum commersonii (Carputo et al 2000), and Solanum cardiophyllum (Thieme et al 2010) were subsequently backcrossed sexually to potato cultivars and exploited in potato breeding programs.…”

Section: Introductionmentioning

confidence: 99%

Genetic composition of interspecific potato somatic hybrids and autofused 4x plants evaluated by DArT and cytoplasmic DNA markers

et al. 2016

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Key message Using DArT analysis, we demonstrated that all Solanum 3 michoacanum (1) S. tuberosum somatic hybrids contained all parental chromosomes. However, from 13.9 to 29.6 % of the markers from both parents were lost in the hybrids. Abstract Somatic hybrids are an interesting material for research of nucleus-cytoplasm interaction and sources of new nuclear and cytoplasmic combinations. Analyses of genomes of somatic hybrids are essential for studies on genome compatibility between species, its evolution and are important for their efficient exploitation. Diversity array technology (DArT) permits analysis of the composition of nuclear DNA of somatic hybrids. The nuclear genome compositions of 97 Solanum 3 michoacanum (?) S. tuberosum [mch (?) tbr] somatic hybrids from five fusion combinations and 11 autofused 4x mch were analyzed for the first time based on DArT markers. Out of 5358 DArT markers generated in a single assay, greater than 2000 markers were polymorphic between parents, of which more than 1500 have a known chromosomal location on potato genetic or physical map. DArT markers were distributed along the entire length of 12 chromosomes. We noticed elimination of markers of wild and tbr fusion components. The nuclear genome of individual somatic hybrids was diversified. Mch is a source of resistance to Phytophthora infestans. From 97 mch (?) tbr somatic hybrids, two hybrids and all 11 autofused 4x mch were resistant to P. infestans. The analysis of the structure of particular hybrids' chromosomes indicated the presence of markers from both parental genomes as well as missing markers spread along the full length of the chromosome. Markers specific to chloroplast DNA and mitochondrial DNA were used for analysis of changes within the organellar genomes of somatic hybrids. Random and nonrandom segregations of organellar DNA were noted.

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Interspecific potato somatic hybrids between Solanum tuberosum and S. cardiophyllum, potential sources of late blight resistance breeding

Cited by 38 publications

References 35 publications

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

Development of polymorphic EST-SSR markers and characterization of the autotetraploid genome of sainfoin (Onobrychis viciifolia)

Genetic composition of interspecific potato somatic hybrids and autofused 4x plants evaluated by DArT and cytoplasmic DNA markers

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