Interstitial assessment of aggressive prostate cancer by physio‐chemical photoacoustics: An <i>ex vivo</i> study with intact human prostates

Huang, Shengsong; Yu, Qin; Chen, Yingna; Pan, Jian; Xu, Chengdang; Wu, Denglong; Chao, Wan-yu; Wei, John T.; Tomlins, Scott A.; Wang, Xueding; Fowlkes, J. Brian; Carson, Paul L.; Cheng, Qian; Xu, Guan

doi:10.1002/mp.13061

Cited by 22 publications

(46 citation statements)

References 37 publications

(92 reference statements)

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“…In the future, the proposed TUT could be integrated in conventional optical-microscopy techniques to allow multimodal microscopy that is capable of both ultrasound stimulation and sensing. Eventually, the TUT technology could be further scaled to develop miniaturized photoacoustic endomicroscopy and microendoscopy devices for space-constrained point-of-care clinical applications, e.g., prostate and pancreas needle biopsies [35][36][37].…”

Section: Discussionmentioning

confidence: 99%

Optical-Resolution Photoacoustic Microscopy Using Transparent Ultrasound Transducer

Chen

Agrawal

Dangi

et al. 2019

Sensors

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The opacity of conventional ultrasound transducers can impede the miniaturization and workflow of current photoacoustic systems. In particular, optical-resolution photoacoustic microscopy (OR-PAM) requires the coaxial alignment of optical illumination and acoustic-detection paths through complex beam combiners and a thick coupling medium. To overcome these hurdles, we developed a novel OR-PAM method on the basis of our recently reported transparent lithium niobate (LiNbO 3 ) ultrasound transducer (Dangi et al., Optics Letters, 2019), which was centered at 13 MHz ultrasound frequency with 60% photoacoustic bandwidth. To test the feasibility of wearable OR-PAM, optical-only raster scanning of focused light through a transducer was performed while the transducer was fixed above the imaging subject. Imaging experiments on resolution targets and carbon fibers demonstrated a lateral resolution of 8.5 µm. Further, we demonstrated vasculature mapping using chicken embryos and melanoma depth profiling using tissue phantoms. In conclusion, the proposed OR-PAM system using a low-cost transparent LiNbO 3 window transducer has a promising future in wearable and high-throughput imaging applications, e.g., integration with conventional optical microscopy to enable a multimodal microscopy platform capable of ultrasound stimulation.

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Section: Discussionmentioning

confidence: 99%

Optical-Resolution Photoacoustic Microscopy Using Transparent Ultrasound Transducer

Chen

Agrawal

Dangi

et al. 2019

Sensors

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“…Magnetic resonance imaging (MRI) has also been used in the assessment of prostate cancer [14–17]. However, patients with prostates that are suspected of being cancerous through MRI assessment still typically require a biopsy for confirmation of the prostate cancer [14, 15, 18]. Thus, there is a need to develop novel techniques for detecting prostate cancers through noninvasive means.…”

Section: Introductionmentioning

confidence: 99%

Photoacoustic imaging of clofazimine hydrochloride nanoparticle accumulation in cancerous vs normal prostates

et al. 2019

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Prostate cancer was the most common form and had the second highest death rate of male cancer in the United States in 2015. Current diagnosis techniques, such as prostate-specific antigen tests, transrectal ultrasound scans, and biopsies, are often inconclusive, and in the latter case, invasive. Here, we explore the use of clofazimine hydrochloride nanoparticles (CFZ-HCl NPs), a repurposed formulation from an FDA-approved antimycobacterial agent, as a photoacoustic contrast agent for the evaluation of prostate cancer due to its macrophage-targeting capabilities and high optical absorbance at 495 nm. Using a transgenic adenocarcinoma of the mouse prostate (TRAMP) mouse model, our results indicate a preferential accumulation of intravenously injected CFZ-HCl NPs in cancerous prostates over normal prostates. Differences in accumulation of CFZ-HCl NPs between cancerous and normal prostates were determined using a two-wavelength unmixing technique via ex vivo photoacoustic imaging. Thus, intravenous injection of CFZ-HCl NPs leads to differences in the interactions of the particles with cancerous vs normal prostates, while allowing for photoacoustic detection and analysis of prostate cancer. These findings could lead to the development of a new noninvasive technique for the detection and monitoring of prostate cancer progression in an animal model that can potentially be translated to human patients.

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“…Angiogenesis and lipid accumulation have been shown to be increased in prostate cancer tumors [58,59]. In vivo [43] and ex vivo [34,35,41] imaging of human prostates have shown differences between malignant and benign prostate tissue using endogenous contrast. We have recently shown that successful prostate cancer biopsy targeting in ex vivo human prostates is possible using 1064 nm PAT and US imaging [34].…”

mentioning

confidence: 99%

“…While PAT has the potential to provide real-time biopsy targets, current PAT setups have not yet demonstrated the ability to image the full posterior-anterior depth as observed from the rectum with the US transducer [34,35,[41][42][43]. Two locations for the placement of the light delivery source for PAT have been investigated.…”

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confidence: 99%

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Cylindrical illumination with angular coupling for whole-prostate photoacoustic tomography

Bungart¹,

Cao²,

Yang-Tran³

et al. 2019

Biomed. Opt. Express

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Current diagnosis of prostate cancer relies on histological analysis of tissue samples acquired by biopsy, which could benefit from real-time identification of suspicious lesions. Photoacoustic tomography has the potential to provide real-time targets for prostate biopsy guidance with chemical selectivity, but light delivered from the rectal cavity has been unable to penetrate to the anterior prostate. To overcome this barrier, a urethral device with cylindrical illumination is developed for whole-prostate imaging, and its performance as a function of angular light coupling is evaluated with a prostate-mimicking phantom.

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Interstitial assessment of aggressive prostate cancer by physio‐chemical photoacoustics: An ex vivo study with intact human prostates

Abstract: The proposed procedure has shown promises in the diagnosis of aggressive prostate cancer.

Cited by 22 publications

References 37 publications

Optical-Resolution Photoacoustic Microscopy Using Transparent Ultrasound Transducer

Optical-Resolution Photoacoustic Microscopy Using Transparent Ultrasound Transducer

Photoacoustic imaging of clofazimine hydrochloride nanoparticle accumulation in cancerous vs normal prostates

Cylindrical illumination with angular coupling for whole-prostate photoacoustic tomography

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