Interstitial Collagenase Is Required for Angiogenesis in Vitro

Fisher, Carolyn U.; Gilbertson-Beadling, S.; Powers, Elaine A.; Petzold, Gary L.; Poorman, Roger A.; Mitchell, Mark A.

doi:10.1006/dbio.1994.1104

Cited by 206 publications

(120 citation statements)

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“…To examine which class of proteolytic enzymes is involved in tube formation in our assay, cultures were treated with aprotinin (bovine pancreas trypsin inhibitor), an inhibitor of plasmin activity, or marimastat, a synthetic low-molecular-weight MMP inhibitor (Wojtowicz-Praga et al, 1997). Treatment with marimastat attenuated the formation of tubelike structures in cultures grown on plastic, collagen type I, or fibrin gel, which is in agreement with Fisher et al (1994), who showed MMP-dependent inhibition of tube formation in endothelial cells grown on collagen type I. In contrast to other models based on fibrin invasion (Jimi et al, 1995;Koolwijk et al, 1996), treatment with aprotinin did not have an effect on endothelial sprouting, regardless of the type of coating used.…”

Section: Effect Of Angiogenic and Antiangiogenic Compoundssupporting

confidence: 87%

“…This might enable interventions of tumor progression by the use of antiangiogenic compounds (Hanahan and Folkman, 1996); eg. directed against endothelial growth factors, growth factor receptors, proteolytic enzymes, or extracellular matrix molecules (Fisher et al, 1994;Ingber, 1992;Millauer et al, 1994). On the other hand, proangiogenic therapy may provide a promising strategy for patients suffering from vascular insufficiency (Rosengart et al, 1999).…”

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confidence: 99%

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Effect of Angiogenic and Antiangiogenic Compounds on the Outgrowth of Capillary Structures from Fetal Mouse Bone Explants

Deckers

Pluijm

Dooijewaard

et al. 2001

Laboratory Investigation

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SUMMARY:Fetal mouse metatarsals are well-known models to study cartilage differentiation and osteoclastic resorption. We show here the outgrowth of PECAM-1 positive tubelike structures from the bone rudiments. This feature can be used to study angiogenesis in vitro. The area of outgrowth significantly increased with culture time, as shown by computerized image analysis of PECAM-1 positive tubelike structures. Treatment with recombinant human vascular endothelial growth factor (rhVEGF-A) stimulated the formation of tubelike structures. Treatment of explants with the angiogenesis inhibitor endostatin, the chemokine IP-10, and the thalidomide derivative phatolyl glutamic acid (PG-acid) resulted in an inhibition of the formation of PECAM-1 positive tubelike structures of 48.8% (Ϯ 4%), 50.2% (Ϯ12%), and 80.8% (Ϯ3%), respectively. Outgrowth of tubelike structures was partly dependent on endogenous VEGF-A because treatment with anti-mVEGF-A and truncated VEGF receptor 1 (soluble fms-like tyrosine kinase 1, sFlt1) strongly inhibited the formation of tubelike structures 74% (Ϯ 4%) and 38% (Ϯ 5%), respectively. Neither onset of tube formation nor total area of tubelike structures were changed when metatarsals were cultured on a fibrin gel or collagen type I gel. Tube formation required activation of matrix metalloproteinases because treatment of the bones with an inhibitor of matrix metalloproteinases completely inhibited migration and tube formation, whereas treatment with an inhibitor of plasmin had no effect. In conclusion, we describe a new in vitro model to study angiogenesis that can be used to test the angiogenic or antiangiogenic potential of novel test compounds that also combines the multicellularity of in vivo assays with the accessibility and flexibility of in vitro assays. (Lab Invest 2001, 81:5-15).

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Section: Effect Of Angiogenic and Antiangiogenic Compoundssupporting

confidence: 87%

mentioning

confidence: 99%

Effect of Angiogenic and Antiangiogenic Compounds on the Outgrowth of Capillary Structures from Fetal Mouse Bone Explants

Deckers

Pluijm

Dooijewaard

et al. 2001

Laboratory Investigation

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“…51 Evidence from TIMPs and synthetic MMP inhibitors MMP inhibition studies support the notion that angiogenesis is dependent at least in part on the actions of MMPs since both TIMPs and synthetic MMP inhibitors, such as BB94 (Batimastat) and BMS-275291, display antiangiogenic properties in vitro and in vivo. [52][53][54] A specific example of this is KB-R7785, a hydroxamate-type metalloproteinase inhibitor that has been shown to inhibit tumor growth and angiogenesis in vivo in the transparent chamber model of tumor progression. 55 The proteolytic activities of the MMPs are considered to be important for EC migration and invasion through the ECM during angiogenesis as several studies have demonstrated TIMP inhibition of EC migration through and on a variety of matrices.…”

Section: Endogenous Inhibitors Of Metalloproteinasesmentioning

confidence: 99%

Metalloproteinases and their inhibitors in tumor angiogenesis

Handsley

Edwards

2005

Intl Journal of Cancer

263

211

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Angiogenesis is the process by which new blood vessels are formed from preexisting vasculature. It is an essential feature of the female reproductive cycle, embryonic development and wound repair. Angiogenesis has also been identified as a causal or contributing factor in several pathologies, including cancer, where it is a rate-limiting step during tumor progression. Matrix metalloproteinases (MMPs) are a family of soluble and membrane-anchored proteolytic enzymes that can degrade components of the extracellular matrix (ECM) as well as a growing number of modulators of cell function. Several of the MMPs, in particular the gelatinases and membrane-type 1 MMP (MT1-MMP), have been linked to angiogenesis. Potential roles for these proteases during the angiogenic process include degradation of the basement membrane and perivascular ECM components, unmasking of cryptic biologically relevant sites in ECM components, modulation of angiogenic factors and production of endogenous angiogenic inhibitors. This review brings together what is currently known about the functions of the MMPs and the closely related ADAM (a disintegrin and metalloproteinase domain) and ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) families in angiogenesis and considers how this information might be useful in manipulation of the angiogenic process, with a view to constraining tumor progression. ' 2005 Wiley-Liss, Inc.Key words: metalloproteinase; tumor; angiogenesis; tissue inhibitor of metalloproteinases; protease; extracellular matrix Angiogenesis is an essential feature of several physiologic processes such as the developmental growth of organs, wound healing and the female reproductive cycle. 1 It is also particularly significant in cancer progression, being a crucial step in the transition of a tumor from a hyperplastic but contained in situ state to a malignant phenotype. 2,3 To allow its growth beyond a certain critical size, a solid avascular tumor must develop its own blood supply in order to meet its growing metabolic requirements. 1 Vascularization of a tumor also provides a route for dissemination of the tumor cells to different sites around the body.Although angiogenesis appears to be the primary form of tumor vascularization, the malignant cells of a tumor can gain access to a blood supply through other means, such as via tumor-induced vasculogenesis, in which bone marrow-derived precursor endothelial cells contribute to the formation of tumor vessels. 4,5 Vasculogenic mimicry is another form of vessel formation that has been reported in certain tumor types, such as aggressive human uveal melanomas, prostate and breast tumors. [6][7][8] In these cases, networks of tumor cell-lined vascular channels were identified within the tumors. Finally, vessel co-option, implemented by tumor cells as a temporary means of gaining access to a blood supply, involves the tumor cells growing around and thus co-opting existing host vessels to form an initially well-vascularized tumor. 9 This review will highlight new insigh...

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“…The latent form (proenzyme) can be activated, at least "in vitro", by other proteases (e.g., plasmin, plasma kallikrein, tissue plasminogen activator, which are present in the inflammatory micro-environment), as well as by mercurials (e.g., 4-aminophenylmercuric acetate) [6]. MMPs appear to be crucial for connective tissue remodelling in physiologic processes, including wound healing [7], angiogenesis [8], cytotrophoblast implantation [9], embryonic development [10], in the cycling of endometrium [11] and in pathologic conditions, such as tumor invasion and metastasis [12], progressive joint destruction [13], inflammation [14], Alzheimer's disease [15] and atherosclerosis [16]. The source of degradative proteinases may depend on the type of disease: in rheumatoid arthritis enzymes may arise from the pannus that proliferates over the cartilage and from inflammatory cells, such as neutrophils and macrophages, that attack the cartilage surface.…”

Section: Introductionmentioning

confidence: 99%

A correlation between knee cartilage degradation observed by arthroscopy and synovial proteinases activities

Marini

Fasciglione

Monteleone

et al. 2003

Clinical Biochemistry

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Objective: A novel study has been carried out to characterize the amount and activity levels of metalloproteinases (i.e., MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 and MMP-13) and of their inhibitors (i.e., TIMP-1 and TIMP-2) in synovial fluid from patients (n ϭ 56) with different degrees of either chondral lesions or knee arthritis identified and classified by arthroscopy. Design and methods: Zymographies, Western blotting and ELISA tests have been used to correlate the disease stage, as determined by arthroscopy, and both the amount and the activation state of different MMPs and of their inhibitors. Results: Analysis of data obtained demonstrates that the degree of cartilage degradation, as seen by arthroscopy, is strictly related to the activity of some synovial MMPs, in particular MMP-2 and MMP-13 and on reduced inhibitory effect of MMP-2 by TIMP-2; in addition, a serine protease weighing about 125 kDa appears only in patients with severe cartilage degradation, i.e., with knee arthritis. Conclusions: On the whole, this is the first study in which an analysis of synovial MMPs/other proteinases activity and TIMPs has been strictly related to arthroscopy results in patients with different degrees of osteoarthritis. Results indicate that an imbalance between specific MMP activities and the amount of TIMPs and of its inhibitory efficiency is crucial for the disease evolution and it is related to the disease stage.

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Interstitial Collagenase Is Required for Angiogenesis in Vitro

Cited by 206 publications

References 0 publications

Effect of Angiogenic and Antiangiogenic Compounds on the Outgrowth of Capillary Structures from Fetal Mouse Bone Explants

Effect of Angiogenic and Antiangiogenic Compounds on the Outgrowth of Capillary Structures from Fetal Mouse Bone Explants

Metalloproteinases and their inhibitors in tumor angiogenesis

A correlation between knee cartilage degradation observed by arthroscopy and synovial proteinases activities

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