Intervenolin, a new antitumor compound with anti-Helicobacter pylori activity, from Nocardia sp. ML96-86F2

Abstract

Because stromal cells can regulate the growth and metastasis of tumor cells, a compound that modulates the interaction between the stromal cells and the tumor cells can control the tumor progression. In the course of our screening for such a compound, we have isolated a new compound, intervenolin, from the culture broth of Nocardia sp. ML96-86F2. Intervenolin inhibits the growth of human gastric and colorectal cancer cell lines in the coculture with the respective organ-derived stromal cells more strongly than that of the cancer cells cultured alone. Intervenolin shows antitumor effect against a xenograft model of human colorectal cancer cells in vivo. Furthermore, intervenolin exerts selective anti-Helicobacter pylori effect.

Chemical constituents and chemotaxonomic study on the marine actinomycete Williamsia sp. MCCC 1A11233

“…While fewer studies were carried out on isolation and purification of chemical constituents from other several genera, including Nocardia, Gordonia, and Tsukamurella (Fu et al, 2011;Kawada et al, 2013;Kobayashi et al, 1997;Sugiyama et al, 2015). In this study, 24 compounds were obtained from Williamsia sp.…”

Monotherapy with a novel intervenolin derivative, AS‐1934, is an effective treatment for Helicobacter pylori infection

“…ITV inhibits the growth of several human cancer cell lines. 11 ITV and its derivatives also exhibit anti-H. pylori activity in vitro. 12,13 Here, we demonstrated that the ITV derivative, AS-1934, showed anti-H. pylori activity including antibiotic-resistant strains in vitro and exerted anti-H. pylori activity in vivo.…”

“…The minimum inhibitory concentrations (MICs, μg/ml) against H. pylori were determined by the micro broth dilution method in 96-well plates as described previously. 11 MICs for aerobic bacteria were determined by the agar dilution streak method recommended by Clinical and Laboratory Standards Institute document M07-A7. 40…”

Intervenolin suppresses gastric cancer cell growth through the induction of TSP‑1 secretion from fibroblast‑like stromal cells

“…In a recent study, we found a novel compound, intervenolin (ITV), from the culture medium of Nocardia sp. Ml96-86F2 (11). ITV inhibited the in vitro growth of human gastric cancer cells.…”