Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups

Silva, Maísa de Lima Correia; Speridião, Patrícia da Graça Leite; Marciano, Renata; Amâncio, Olga Maria Silvério; Morais, Tania Beninga; Morais, Mauro Batista de

doi:10.1590/1678-98652017000100002

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…Another point of equal importance is the fact that this is the first study that compares SB in relation to CM with and without the addition of FOS for a continuous period sufficient to promote significant changes in the nutritional state of the animal. This study used an experimental model previously developed in our laboratory that allowed us to evaluate the nutritional interactions in recently weaned rats exclusively fed on different types of liquid formulas ( 29 , 30 , 37 , 38 ) . During the experiment, the animals did not receive solid feed, with their food intake limited to SB and CM, according to the study group.…”

Section: Discussionmentioning

confidence: 99%

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Effect of fructo-oligosaccharide supplementation in soya beverage on the intestinal absorption of calcium and iron in newly weaned rats

et al. 2018

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Studies have shown the positive effects of prebiotics on the intestinal absorption of Ca and Fe. The present study evaluated the effect of fructo-oligosaccharide (FOS) supplementation in soya beverage (SB) on absorption mechanisms of Ca and Fe in recently weaned rats. Male Wistar rats were divided into four groups: lactose-free cows’ milk (CM), lactose-free CM with FOS (0·8 g/100 ml) (CMF), SB and soya beverage with FOS (0·8 g/100 ml) (SBF). These rats were euthanised after 1 week of treatment. Organ weight, pH of the caecal content and absorption mechanisms of Ca and Fe were evaluated. The results showed that the weight of the caecal contents increased in the CMF and SBF groups, and the pH of the caecal contents was lower in these groups. The Hb levels of the CMF and SB groups were higher when compared with that of the CM group and lower in relation to the SBF group. The apparent Ca and Fe absorption and apparent Ca retention in the CM group were higher when compared with the SB group, whereas in the CMF group, they were higher in relation to the SBF group. Divalent metal transporter 1 (DMT1) protein expression in the duodenum was higher in the SBF group than in the SB and CMF groups. SB resulted in lower intestinal Ca absorption and higher Hb concentration, despite the lower apparent Fe absorption in relation to CM. Supplementation with FOS provided beneficial effects on Hb and DMT1 protein expression in the duodenum, in addition to improving the absorption process.

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Section: Discussionmentioning

confidence: 99%

“…American Institute of Nutrition-93G (AIN-93G)) because the objective of the study was to analyse the interaction of foods (CM, SB and FOS) to simulate what would occur in humans. That is, the feeding strategy was not intended to meet the needs of the animals involved in the experiment ( 29 , 30 , 37 , 38 ) .…”

Section: Discussionmentioning

confidence: 99%

Effect of fructo-oligosaccharide supplementation in soya beverage on the intestinal absorption of calcium and iron in newly weaned rats

et al. 2018

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“…This study concluded that soymilk and iron-fortified cow milk were able to prevent anemia in those young, despite the lower amount of inorganic iron contained in soymilk [123]. Similar results were obtained when comparing intestinal iron absorption derived from cow milk or soymilk in weaning rats [124].…”

Section: Nutritional Resources For Iron Managementmentioning

confidence: 52%

Iron-Enriched Nutritional Supplements for the 2030 Pharmacy Shelves

et al. 2021

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Iron deficiency (ID) affects people of all ages in many countries. Due to intestinal blood loss and reduced iron absorption, ID is a threat to IBD patients, women, and children the most. Current therapies can efficiently recover normal serum transferrin saturation and hemoglobin concentration but may cause several side effects, including intestinal inflammation. ID patients may benefit from innovative nutritional supplements that may satisfy iron needs without side effects. There is a growing interest in new iron-rich superfoods, like algae and mushrooms, which combine antioxidant and anti-inflammatory properties with iron richness.

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Calcium is a noncompetitive inhibitor of DMT1 on the intestinal iron absorption process: empirical evidence and mathematical modeling analysis

Cegarra

Aguirre

Núñez

et al. 2022

American Journal of Physiology-Cell Physiology

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Iron absorption is a complex and highly controlled process where DMT1 transports nonheme iron through the brush border membrane of enterocytes to the cytoplasm but does not transport alkaline-earth metals such as calcium. However, it has been proposed that high concentrations of calcium from the diet could reduce iron bioavailability. In this work we investigate the effect of intracellular and extracellular calcium on iron uptake by Caco-2 cells, as determined by calcein fluorescence quenching. We found that extracellular calcium inhibits iron uptake by Caco-2 cells in a concentration-dependent manner. Chelation of intracellular calcium with BAPTA did not affect iron uptake, which indicates that the inhibitory effect of calcium is not exerted through intracellular calcium signaling. Kinetic studies performed, provided evidence that calcium acts as a reversible non-competitive inhibitor of the iron transport activity of DMT1. Based on these experimental results, a mathematical model was developed that considers the dynamics of non-competitive inhibition using a four-state mechanism to describe the inhibitory effect of calcium on the DMT1 iron transport process in intestinal cells. The model accurately predicts the calcein fluorescence quenching dynamics observed experimentally after an iron challenge. Therefore, the proposed model structure is capable of representing the inhibitory effect of extracellular calcium on DMT1-mediated iron entry into the cLIP of Caco-2 cells. Considering the range of calcium concentrations that can inhibit iron uptake, the possible inhibition of dietary calcium on intestinal iron uptake is discussed.

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Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups

Cited by 3 publications

References 29 publications

Effect of fructo-oligosaccharide supplementation in soya beverage on the intestinal absorption of calcium and iron in newly weaned rats

Effect of fructo-oligosaccharide supplementation in soya beverage on the intestinal absorption of calcium and iron in newly weaned rats

Iron-Enriched Nutritional Supplements for the 2030 Pharmacy Shelves

Calcium is a noncompetitive inhibitor of DMT1 on the intestinal iron absorption process: empirical evidence and mathematical modeling analysis

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