Intestinal anti-inflammatory effects of total alkaloid extract from Fumaria capreolata in the DNBS model of mice colitis and intestinal epithelial CMT93 cells

Bribi, Noureddine; Algieri, Francesca; Rodríguez-Nogales, Alba; Vezza, Teresa; Garrido-Mesa, José; Utrilla, M. Pilar; Contreras, María del Mar; Maiza, Fadila; Segura‐Carretero, Antonio; Gálvez, Júlio

doi:10.1016/j.phymed.2016.05.003

Cited by 29 publications

(24 citation statements)

References 37 publications

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“…In particular, the analysis of specific cytokine changes related to various T‐cell subsets could elucidate key mechanisms for the beneficial effects of the PTSO treatment. In the present study, both experimental models of colitis were associated with the upregulation of proinflammatory cytokines in the inflamed colonic tissue, in accordance with previous studies . The treatment with PTSO resulted in a reduction of proinflammatory cytokines such as IL‐1β, Tnf‐α, IL‐6 , and IL‐17 .…”

Section: Discussionsupporting

confidence: 92%

“…As previously reported, the inflammatory process induced by DNBS was associated with an altered expression of the pro‐inflammatory markers evaluated by RT‐qPCR. Therefore, the expression of the pro‐inflammatory cytokines evaluated ( IL‐1β , Tnf‐α , IL‐6 , and IL‐17 ) was significantly increased in the DNBS colitic group in comparison with the noncolitic.…”

Section: Resultssupporting

confidence: 79%

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The Immunomodulatory Properties of Propyl‐Propane Thiosulfonate Contribute to its Intestinal Anti‐Inflammatory Effect in Experimental Colitis

Vezza

Algieri

Garrido-Mesa

et al. 2019

Molecular Nutrition Food Res

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Scope Propyl‐propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti‐inflammatory, immunomodulatory, and antimicrobial properties. In consequence, PTSO can be a potential candidate for the treatment of inflammatory bowel diseases. Methods and results The anti‐inflammatory effects of PTSO are studied in two mice models of colitis: 2,4‐dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01–10 mg kg–1) and dextran sodium sulfate (DSS) (PTSO doses: 0.01–0.1 mg kg–1). The immunomodulatory effects of PTSO (0.1–25 µm) are also shown in vitro in Caco‐2 and THP‐1 cells, reducing the production of pro‐inflammatory mediators and downregulating mitogen‐activated protein kinases (MAPKs) signaling pathways. This compound displays beneficial effects in both models of mouse colitis by reducing the expression of different pro‐inflammatory mediators and improving the intestinal epithelial barrier integrity. Moreover, PTSO ameliorates the altered gut microbiota composition observed in DSS colitic mice. Conclusion PTSO exerts intestinal anti‐inflammatory activity in experimental colitis in mice. This anti‐inflammatory activity can be associated with the immunomodulatory properties of PTSO through the regulation of the activity of cells involved in the inflammatory response. Furthermore, PTSO is able to restore the intestinal epithelial barrier function and to ameliorate the intestinal microbiota homeostasis, thus supporting its future development in human IBD.

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Section: Discussionsupporting

confidence: 92%

Section: Resultssupporting

confidence: 79%

The Immunomodulatory Properties of Propyl‐Propane Thiosulfonate Contribute to its Intestinal Anti‐Inflammatory Effect in Experimental Colitis

Vezza

Algieri

Garrido-Mesa

et al. 2019

Molecular Nutrition Food Res

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“…Protopine exhibits anti-inflammatory activity both in vitro and in vivo [ 40 , 41 ]. This alkaloid can contribute, at least in part, to explaining the antinociceptive and anti-inflammatory effects of the F. capreolata extract showed in our previous studies [ 2 , 6 , 7 ]. Thus, for the quality control of this alkaloid extract, protopine was preliminary selected as analytical/active marker since it is also commercially available and the RP-HPLC method was selected since it was shorter than the GC method.…”

Section: Resultsmentioning

confidence: 88%

“…In fact, alkaloids, including the aforementioned type, have recently been reviewed to be effective treatments of intestinal inflammation injury in animal models [ 5 ]. Interestingly, recent studies have shown the low toxicity of a Fumaria capreolata alkaloid extract (white ramping-fumatory) in vitro and in vivo [ 2 ], as well as their intestinal anti-inflammatory effects in mice colitis [ 6 ] and antinociceptive activity [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry

Contreras

Bribi

Gómez-Caravaca

et al. 2017

International Journal of Analytical Chemistry

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Two analytical platforms, gas chromatography (GC) coupled to quadrupole-time-of-flight (QTOF) mass spectrometry (MS) and reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled to diode array (DAD) and QTOF detection, were applied in order to study the alkaloid profile of Fumaria capreolata. The use of these mass analyzers enabled tentatively identifying the alkaloids by matching their accurate mass signals and suggested molecular formulae with those previously reported in libraries and databases. Moreover, the proposed structures were corroborated by studying their fragmentation pattern obtained by both platforms. In this way, 8 and 26 isoquinoline alkaloids were characterized using GC-QTOF-MS and RP-UHPLC-DAD-QTOF-MS, respectively, and they belonged to the following subclasses: protoberberine, protopine, aporphine, benzophenanthridine, spirobenzylisoquinoline, morphinandienone, and benzylisoquinoline. Moreover, the latter analytical method was selected to determine at 280 nm the concentration of protopine (9.6 ± 0.7 mg/g), a potential active compound of the extract. In conclusion, although GC-MS has been commonly used for the analysis of this type of phytochemicals, RP-UHPLC-DAD-QTOF-MS provided essential complementary information. This analytical method can be applied for the quality control of phytopharmaceuticals containing Fumaria extracts currently found in the market.

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“…Various species have been reported for their use in traditional medicine for the treatment of hepatobiliary pathologies [ 5 ]. Other sources cite the antifungal [ 6 ], antibacterial [ 7 ], and anti-inflammatory [ 8 ] activities of these species. These activities are especially due to the isoquinolinic alkaloids, among which protopine is the most frequently found [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Polyphenolic Content, Antioxidant and Diuretic Activities of Six Fumaria Species

et al. 2017

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Romanian traditional medicine describes the use of aerial parts of Fumaria species to treat hepatobiliary diseases as well as diuretic agents. The present study aims to investigate the chemical composition, antioxidant properties, and diuretic effects of several Fumaria species. LC/MS analysis revealed that Fumaria species contain phenolic acids and high amounts of flavonoids with rutin and isoquercitrin as main compounds. Concerning antioxidant capacity, the most significant results were obtained for F. capreolata and F. vailantii. Both species showed a good correlation between the antioxidant capacity and a high amount of flavonoids. Furthermore, the extracts of F. officinalis and F. schleicheri produced a strong increase in urinary volumetric excretion of saline-loaded rats, 24 h after the oral administration of a single dose of 250 mg/kg bw. Moreover, both extracts of F. officinalis and F. schleicheri increased the urinary excretion of Na+ and K+. Results from the present study offer a new perspective concerning the chemical composition and bioactivities of traditionally used fumitory species.

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Intestinal anti-inflammatory effects of total alkaloid extract from Fumaria capreolata in the DNBS model of mice colitis and intestinal epithelial CMT93 cells

Cited by 29 publications

References 37 publications

The Immunomodulatory Properties of Propyl‐Propane Thiosulfonate Contribute to its Intestinal Anti‐Inflammatory Effect in Experimental Colitis

The Immunomodulatory Properties of Propyl‐Propane Thiosulfonate Contribute to its Intestinal Anti‐Inflammatory Effect in Experimental Colitis

Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry

Evaluation of Polyphenolic Content, Antioxidant and Diuretic Activities of Six Fumaria Species

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