Intestinal epithelium of juvenile douradoSalminus brasiliensis(Cuvier, 1816) fed diet with lyophilized bovine colostrum

Abstract

The aim of this study was to evaluate the effect of lyophilized bovine colostrum (LBC) used as partial source of dietary protein on the histological characteristics of the intestinal epithelium of juvenile dourado Salminus brasiliensis. Juveniles were fed diets containing 0%, 10% or 20% of LBC inclusion for either 30 or 60 days. For the histological study, the intestine was divided into three segments, S1, S2 and posterior intestine. In the S1 segment, interaction between treatment and period was observed in the number of goblet cells containing sialomucin, effect of treatment in the total number of goblet cells and effect of period in the number of goblet cells containing sulphomucins (P < 0.05). In the S2 segment, effect of period was observed in the number of goblet cells containing acid, neutral and total mucins, sialomucins and the partial volume of the absorptive mucosa (Vv) (P < 0.05). In the posterior intestine, effect of period was observed in the thickness of muscle layer and number of goblet cells containing sialomucins and sulphomucins (P < 0.05). Considering the aspects studied, the presence of 0%, 10% or 20% of LBC in the diet did not significantly influence the enteric histological characteristics of juvenile dourado during the period of the study.

Partial substitution of fishmeal with soybean protein-based diets for dourado Salminus brasiliensis

“…Sampled excisions were processed using standard histological techniques (Cruz et al, 2014;Moretti et al, 2014); six blade-mounted sections per fish were stained with haematoxylin and eosin (H.E.). The morphometric analysis of proximal intestine comprised: measurement of mucosal fold height (FH), fold thickness (FT), and muscular layer thickness (MT).…”

Effects of yeast based toxin binder supplementation on growth performance and intestinal microarchitecture in male buffalo calves (Bubalus bubalis) exposed to different concentrations of Aflatoxin B1.

“…Mucins secreated by goblet cells are either acidic, neutral or mixed in nature. In fish GC having acidic mucins protect intestinal epithelium against chemical agents while GC having neutral mucins provide protection against chemical agents (Cruz et al, 2014). Factors that lead to an increase in GC having acidic and neutral mucin under the influence of AFB1 or YTB supplementation are yet to be investigated.…”

Cellular activity and development of enteric, hepatic and muscle tissues of juvenile pacuPiaractus mesopotamicus(Holmberg 1887) fed with lyophilized bovine colostrum

“…Colostrum is a rich source of protein like immunoglobulins, essential nutrients, hormones and biologically active molecules and is widely used in mammals as a source of nutrition and immune protection to newborn (Blum & Baumrucker ; Hurley & Theil ; Moretti, Nordi, Lima, Pauletti, Susin & Machado‐Neto ; Lima, Moretti, Nordi, Pauletti, Susin & Machado‐Neto ; Moretti, Nordi, Lima, Pauletti & Machado‐Neto ). The consumption of bovine colostrum by fish provided intestinal morphometrical alteration, as a consequence of increased activity of absorption processes (Rodrigues, Pauletti, Kindlein, Delgado, Cyrino & Machado‐Neto ), changes in pattern of goblet cells present in enteric mucosae (Cruz, Moretti, Nordi, Cyrino & Machado‐Neto ), the indicators of cellular activity in muscle, liver and intestine tissue (Pauletti, Kindlein, Bagaldo, Rodrigues, Delgado, Cyrino & Machado‐Neto ,b) and was found too absence of implications in intestinal enzymatic activity (Moretti, Nordi, Cruz, Cyrino & Machado‐Neto ).…”