Intestinal Flora Mediates Antiobesity Effect of Rutin in High‐Fat‐Diet Mice

Abstract

Scope Intestinal flora plays a critical role in the development of . Rutin is a natural flavonoid with potential prebiotic effects on regulating the intestinal flora composition that is beneficial for host health. Therefore, this study hypothesizes that rutin supplementation has beneficial effects on high‐fat‐diet (HFD)‐induced obesity and metabolic disorder through the modulation of intestinal flora in mice. Methods and results The obesity‐alleviating property of rutin using 6‐week‐old C57BL/6J male mice fed on HFD with or without rutin supplementation for 16 weeks is investigated. Rutin supplementation effectively reduces body‐weight gain, insulin resistance, and acted favorably on the intestinal barrier, thereby reducing endotoxemia and systemic inflammation. Sequencing of 16S rRNA genes from fecal samples indicate that rutin exerted modulatory effects on HFD‐induced intestinal flora disorders (e.g., rutin decreased Firmicutes abundance and increased Bacteroidetes and Verrucomicrobia abundance). Antibiotic treatment and fecal microbiota transplantation further demonstrate that the salutary effects of rutin on obesity control are strongly dependent on the intestinal flora. Conclusion Rutin can be considered as a prebiotic agent for improving intestinal flora disorders and obesity‐associated metabolic perturbations in obese individuals.

Identifying Ferroptosis‐Related Genes Associated with Weight Loss Outcomes and Regulation of Adipocyte Microenvironment

“…[62] Therefore, some studies suggest appropriate rutin supplementation, which facilitates the protection of the intestinal barrier, thereby reducing systemic inflammation and achieving effective weight control. [63,64] Our results also showed that menadione could bind to NQO1 and affect the function of NQO1, potentially act through blocking NQO1/GPX4 to mediate ferroptosis. [65] Similar studies have found that NQO1 and Menadione affect each other's activity and effects.…”

Hepatoenteric Protective Effect of Melanin from Inonotus hispidus on Acute Alcoholic Liver Injury in Mice

“…The PVDF membranes were treated with chemiluminescence detection reagent and wrapped with plastic wrap, and then the protein bands were observed. [29] 2.5. Intestinal Flora Analysis…”

Natural compounds as obesity pharmacotherapies

“…for 16 weeks (Tung et al, 2021) and 16.5% less BW after rutin treatment at 100 mg/kg/day by i.g. for 16 weeks (Yan et al, 2022). HFD-fed mice gained 12.8% less BW after consuming rutin in drinking water (1 mg/mL) for 10 weeks (Yuan et al, 2017).…”

“…Rutin could reduce lipid accumulation in the adipocytes in vitro by acting as the PPARα/γ dual agonist (Mandal et al, 2022) and in hepatocytes in vitro by decreasing lipogenesis and oxidative stress through AMPK activation (Wu et al, 2011). Additionally, rutin improves gut microbiota dysbiosis in HFD‐fed rats (Peng et al, 2020) and mice (Guo et al, 2018; Yan et al, 2022) and attenuates the systematic, hepatic (Panchal et al, 2011) and intestinal inflammatory in HFD‐fed mice (Guo et al, 2018). Furthermore, rutin restores the gut barrier and downregulates the NF‐kB pathway through the inhibition of toll‐like receptor‐4/‐9 (Yan et al, 2022).…”

“…Additionally, rutin improves gut microbiota dysbiosis in HFD‐fed rats (Peng et al, 2020) and mice (Guo et al, 2018; Yan et al, 2022) and attenuates the systematic, hepatic (Panchal et al, 2011) and intestinal inflammatory in HFD‐fed mice (Guo et al, 2018). Furthermore, rutin restores the gut barrier and downregulates the NF‐kB pathway through the inhibition of toll‐like receptor‐4/‐9 (Yan et al, 2022).…”