Intestinal permeability and fecal eosinophil-derived neurotoxin are the best diagnosis tools for digestive non-IgE-mediated cow’s milk allergy in toddlers

Kalach, Nicolas; Kapel, Nathalie; Waligora‐Dupriet, Anne‐Judith; Castelain, Marie-Christine; Cousin, Marie Odile; Sauvage, C.; Bâ, F.; Nicolis, Ioannis; Campeotto, Florence; Mj, Butel; Dupont, Christophe

doi:10.1515/cclm-2012-0083

Cited by 45 publications

(54 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Accordingly, the levels of fecal EDN may not be sufficiently elevated in samples collected just after ingesting the causative food or at an acute FPIES reaction. On the other hand, levels of fecal EDN before oral food challenges were variable in our patients, which was consistent with a previous report (8). Increased levels of baseline fecal EDN in some patients may be attributable to a direct consequence of previous FPIES episodes or non-specific intestinal inflammation due to previous FPIES episodes.…”

Section: Approval For the Study Was Obtained From The Human Research supporting

confidence: 91%

Increased expression of epidermal thymic stromal lymphopoietin in inflammatory peeling skin syndrome

Wada

Toma

Hamaguchi

et al. 2014

The Journal of Dermatology

View full text Add to dashboard Cite

Section: Approval For the Study Was Obtained From The Human Research supporting

confidence: 91%

Increased expression of epidermal thymic stromal lymphopoietin in inflammatory peeling skin syndrome

Wada

Toma

Hamaguchi

et al. 2014

The Journal of Dermatology

View full text Add to dashboard Cite

“…The in vivo model still needs to be implemented, as it allows the study of mechanisms of action of probiotics, which are known to be multifactorial, including modulation of the host's microbiota, modulation of the immune system, and also improvement of the barrier function of the gut mucosa. Indeed, probiotic strains may also act in parallel on intestinal permeability, which is increased in allergic children (47). Nebot-Vivinus et al showed that the multispecies probiotic Lactibiane Tolerance increased occluding and ZO-1 expression to prevent the disruption of the epithelial barrier (48), which protects the interaction between enteric bacteria and metabolically associated components that can trigger an immune response (49).…”

Section: Discussionmentioning

confidence: 99%

Three Novel Candidate Probiotic Strains with Prophylactic Properties in a Murine Model of Cow's Milk Allergy

Neau

Delannoy

Marion

et al. 2016

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

e Food allergies can have significant effects on morbidity and on quality of life. Therefore, the development of efficient approaches to reduce the risk of developing food allergies is of considerable interest. The aim of this study was to identify and select probiotic strains with preventive properties against allergies using a combination of in vitro and in vivo approaches. To that end, 31 strains of bifidobacteria and lactic acid bacteria were screened for their immunomodulatory properties in two cellular models, namely, human peripheral blood mononuclear cells (PBMCs) and T helper 2 (Th2)-skewed murine splenocytes. Six strains inducing a high interleukin-10 (IL-10)/IL-12p70 ratio and a low secretion of IL-4 on the two cellular models were selected, and their protective impact was tested in vivo in a murine model of food allergy to ␤-lactoglobulin. Three strains showed a protective impact on sensitization, with a decrease in allergen-specific IgE, and on allergy, with a decrease in mast cell degranulation. Analysis of the impact of these three strains on the T helper balance revealed different mechanisms of action. The Lactobacillus salivarius LA307 strain proved to block Th1 and Th2 responses, while the Bifidobacterium longum subsp. infantis LA308 strain induced a pro-Th1 profile and the Lactobacillus rhamnosus LA305 strain induced pro-Th1 and regulatory responses. These results demonstrate that a combination of in vitro and in vivo screening is effective in probiotic strain selection and allowed identification of three novel probiotic strains that are active against sensitization in mice. Food allergies (FA) are an important public health problem that affects adults and children. They have dramatically and rapidly increased in prevalence in the last several decades, especially in areas with a westernized lifestyle. Despite the associated risk of severe allergic reactions and even death, there is currently no treatment for FA; thus, management of the disease continues to be based on patient education, ensuring strict allergen avoidance, and treatment of symptoms (1). Therefore, the development of new strategies for FA prevention appears to be essential to address this unmet medical need.The increase in the prevalence of allergic diseases over the past 20 to 30 years and the dichotomy in their rates between industrialized and developing countries are two lines of evidence suggesting that environmental changes are a major factor for the development of allergies. Intestinal commensal bacteria and their sequential establishment are known to play a crucial role in the maturation of the intestinal immune system, modulation of the T helper (Th) balance, acquisition of oral tolerance, and maintenance of gut wall epithelial integrity (2, 3). Yet, numerous clinical and experimental studies have reported intestinal dysbiosis in patients with allergic symptoms, with a low diversity and qualitative and/or quantitative differences in the composition of their intestinal microbiota compared to that of healthy individuals (4...

show abstract

“…Total DNA from cecal or fecal contents (2 droppings) was extracted using guanidium thiocyanate and the mechanical bead beating method as described previously (26).…”

Section: Methodsmentioning

confidence: 99%

“…Standard curves were obtained from serial dilutions of a known concentration of plasmid DNA containing a 16S rRNA gene insert from each genera or group. The coefficients of correlation between log10 colony-forming units (CFU) and rRNA gene copy numbers for each genera and group were obtained from rrnDB (26), enabling calculation of the number of CFU per gram of cecal content or feces.…”

Section: Methodsmentioning

confidence: 99%

Disturbed intestinal nitrogen homeostasis in a mouse model of high-fat diet-induced obesity and glucose intolerance

Huong

Hindlet

Waligora‐Dupriet

et al. 2014

American Journal of Physiology-Endocrinology and Metabolism

Self Cite

View full text Add to dashboard Cite

Previous studies demonstrated that PEPT1 expression and function in jejunum are reduced in diabetes and obesity, suggesting a nitrogen malabsorption from the diet. Surprisingly, we reported here a decrease in gut nitrogen excretion in high-fat diet (HFD)-fed mice and further investigated the mechanisms that could explain this apparent contradiction. Upon HFD, mice exhibited an increased concentration of free amino acids (AAs) in the portal vein (60%) along with a selective increase in the expression of two AA transporters (Slc6a20a, Slc36a1), pointing to a specific and adaptive absorption of some AAs. A delayed transit time (ϩ40%) and an increased intestinal permeability (ϩ80%) also contribute to the increase in nitrogen absorption. Besides, HFD mice exhibited a 2.2-fold decrease in fecal DNA resulting from a reduction in nitrogen catabolism from cell desquamation and/or in the intestinal microbiota. Indeed, major quantitative (2.5-fold reduction) and qualitative alterations of intestinal microbiota were observed in feces of HFD mice. Collectively, our results strongly suggest that both increased AA transporters, intestinal permeability and transit time, and changes in gut microbiota are involved in the increased circulating AA levels. Modifications in nitrogen homeostasis provide a new insight in HFD-induced obesity and glucose intolerance; however, whether these modifications are beneficial or detrimental for the HFD-associated metabolic complications remains an open issue.

show abstract

Intestinal permeability and fecal eosinophil-derived neurotoxin are the best diagnosis tools for digestive non-IgE-mediated cow’s milk allergy in toddlers

Abstract: In this routine diagnosis allergy work-up for CMA in toddlers, the best efficacy was seen for intestinal permeability compared to IgE, IgG, skin prick test and atopy patch test. Moreover, fecal EDN in a single spot sample displayed a similar performance.

Cited by 45 publications

References 29 publications

Increased expression of epidermal thymic stromal lymphopoietin in inflammatory peeling skin syndrome

Increased expression of epidermal thymic stromal lymphopoietin in inflammatory peeling skin syndrome

Three Novel Candidate Probiotic Strains with Prophylactic Properties in a Murine Model of Cow's Milk Allergy

Disturbed intestinal nitrogen homeostasis in a mouse model of high-fat diet-induced obesity and glucose intolerance

Contact Info

Product

Resources

About