Intestinal permeability and transport of apigenin across Caco-2 cell monolayers

Marina, Z.; Ismail, Amin; Loh, Su Peng; Jailani, Fadhilah; Kartinee, Kassim Nur

doi:10.31665/jfb.2019.7198

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…These results suggest possible differences in matrix-dependent transport efficacy, similar to findings from the study by Hilary et al (2020), which showed differences in transport efficacy between different forms of date seeds. The disparities in transport efficiency between phenolic compounds can be attributed to the chemical structure and solubility of the compounds as well as differences in the transport mechanism (Fang et al, 2017;Marina et al, 2019). The transportation of simple phenolic acids, such as protocatechuic acid and vanillic acid, and flavonoid aglycones, such as chrysoeriol, is likely facilitated by direct passive diffusion.…”

Section: B Tracementioning

confidence: 99%

Effect of gastrointestinal digestion on the stability, antioxidant activity, and Caco‐2 cellular transport of pigmented grain polyphenols

Ed Nignpense,

Francis,

Blanchard

et al. 2024

Journal of Food Science

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Grain polyphenols are known to possess several health properties. However, their digestive stability and intestinal absorption have not been fully elucidated. This study investigated the fate of pigmented grain polyphenols in the digestive system. Purple rice, purple barley, purple wheat, and blue wheat extracts were subjected to simulated gastric and intestinal phase digestion, followed by Caco‐2 cellular transport. Phenolic profiling and antioxidant activity were determined using benchtop assays and an ultra‐high‐performance liquid chromatography–2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid free radical) (UHPLC‐ABTS•+) system. The results demonstrated a decrease in the total phenolic content of extracts after digestion, with purple rice extract retaining the highest phenolic content (79%) and ABTS•+antioxidant activity (31%). Antioxidant activity was retained the most during the gastric phase; however, dominant antioxidant compounds were not detected after intestinal digestion. Significant variations in phenolic composition and radical scavenging activity were detected after digestion. Protocatechuic acid, vanillic acid, apigenin, and chrysoeriol were all transported across the intestinal barrier. The findings of this study provide novel insights into the in vitro stability and antioxidant activity of cereal grain polyphenols after simulated digestion.

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Section: B Tracementioning

confidence: 99%

Effect of gastrointestinal digestion on the stability, antioxidant activity, and Caco‐2 cellular transport of pigmented grain polyphenols

Ed Nignpense,

Francis,

Blanchard

et al. 2024

Journal of Food Science

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“…Many efforts have been made in unrevealing the digestion and absorption of phenolic compounds [ 21 , 22 ], all of which help in better understanding their systemic effects as non-metabolized and/or non-absorbed phenolics in exerting their effects locally upon gastric digestion [ 2 ]. Furthermore, lipase and alpha-glucosidase are involved in the digestion of triacylglycerols and carbohydrates, respectively [ 19 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Do Flavonoids from Durum Wheat Contribute to Its Bioactive Properties? A Prospective Study

et al. 2021

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A clear gap with respect to the potential biological properties of wheat flavonoids exists in the available literature. This information is crucial for breeding programs aiming to produce new varieties presenting improved health benefits. Accordingly, advanced breeding lines of whole durum wheat were evaluated in this contribution. The highest recovery of phenolics was achieved using aqueous acetone (50:50, v/v), as verified by multi-response optimization, thus showing that phenolics could be largely underestimated by employing an inappropriate extraction. The concentration of derivatives of apigenin, the main phenolics present, ranged from 63.5 to 80.7%, as evaluated by LC–ESI-QTOF-MS. Phenolics from the breeding line 98 exhibited the highest ability in scavenging peroxyl radicals, reducing power as well as in terms of inhibition of pancreatic lipase activity, a key enzyme regulating the absorption of triacylglycerols. In contrast, none of the samples exhibited a significant anti-diabetic potential. Despite their high concentration compared to that of phenolic acids, results of this work do not support a significant antioxidant and pancreatic lipase inhibitory effect of durum wheat flavonoids. Therefore, breeding programs and animal and/or human trials related to the effect of durum wheat flavonoids on oxidative stress and absorption of triacylglycerols are discouraged at this point.

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Anti-inflammatory effect of lentil hull (Lens culinaris) extract via MAPK/NF-κB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture

Guo

Pei

et al. 2022

Journal of Functional Foods

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Intestinal permeability and transport of apigenin across Caco-2 cell monolayers

Cited by 3 publications

References 16 publications

Effect of gastrointestinal digestion on the stability, antioxidant activity, and Caco‐2 cellular transport of pigmented grain polyphenols

Effect of gastrointestinal digestion on the stability, antioxidant activity, and Caco‐2 cellular transport of pigmented grain polyphenols

Do Flavonoids from Durum Wheat Contribute to Its Bioactive Properties? A Prospective Study

Anti-inflammatory effect of lentil hull (Lens culinaris) extract via MAPK/NF-κB signaling pathways and effects of digestive products on intestinal barrier and inflammation in Caco-2 and Raw264.7 co-culture

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