Intestinal Tract Microbe Communities Associated with Horseshoe Crabs from Beibu Gulf, China

Wang, Pei; Ning, Yi; Huang, Jingcai; Dai, Zhicheng; Wang, Hong; Wang, Yujun; Liao, Yongyan

doi:10.1007/s00284-020-02140-x

Cited by 7 publications

(4 citation statements)

References 36 publications

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“…32,33 For example, the use of sulfolane as a supercharging reagent, 34 permitted the differentiation of lasso and branched-cyclic topoisomers at high charge states, which was not observed for lower charged species (Figure S2). 26,27,35 The push for higher resolution and sensitivity has led to the developments of various forms of IMS devices, that can be typically classified as scanning (e.g., FAIMS, 36 DMA, 37 and transverse modulation IMS 38 ) or dispersive (e.g., drift-tube IMS, 39 periodic focusing drift tube IMS, 40 TWIMS, 41 overtone mobility IMS, 42 cyclic TWIMS, 43 and SLIMS 44 ). In particular, the recent introduction of trapped IMS (TIMS) and its integration with MS analyzers, 45−50 has found multiple applications in analytical workflows with high mobility resolving power (R up to ∼400) in a compact geometry.…”

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confidence: 99%

Identification of Lasso Peptide Topologies Using Native Nanoelectrospray Ionization-Trapped Ion Mobility Spectrometry–Mass Spectrometry

et al. 2018

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Lasso peptides are a fascinating class of bioactive ribosomal natural products characterized by a mechanically interlocked topology. In contrast to their branched-cyclic forms, lasso peptides have higher stability and have become a scaffold for drug development. However, the identification and separation of lasso peptides from their unthreaded topoisomers (branched-cyclic peptides) is analytically challenging since the higher stability is based solely on differences in their tertiary structures. In the present work, a fast and effective workflow is proposed for the separation and identification of lasso from branched cyclic peptides based on differences in their mobility space under native nanoelectrospray ionization-trapped ion mobility spectrometry-mass spectrometry (nESI-TIMS-MS). The high mobility resolving power ( R) of TIMS resulted in the separation of lasso and branched-cyclic topoisomers ( R up to 250, 150 needed on average). The advantages of alkali metalation reagents (e.g., Na, K, and Cs salts) as a way to increase the analytical power of TIMS is demonstrated for topoisomers with similar mobilities as protonated species, efficiently turning the metal ion adduction into additional separation dimensions.

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confidence: 99%

Identification of Lasso Peptide Topologies Using Native Nanoelectrospray Ionization-Trapped Ion Mobility Spectrometry–Mass Spectrometry

et al. 2018

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“…This study also investigates the potential production of tetrodotoxin by its symbiotic microorganisms. In recent, two studies reported on gut microbiome in early stages (first and second instar) of cultivated T. tridentatus (Miao et al, 2020) and wild adults of T. tridentatus and C. rotundicauda (Wang et al, 2020), and this study provides the first result revealing the diversity and variations of gut microbiota in wild juveniles of the two Asian horseshoe crab species. We then analyzed if any species of bacteria known to produce tetrodotoxin (Magarlamov et al, 2017) was present in the gut of horseshoe crabs.…”

Section: Discussionmentioning

confidence: 59%

Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda

Leung

Nong

et al. 2020

Front. Mar. Sci.

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Horseshoe crabs are a group of marine chelicerates that contain only four extant species, some of which are endangered. Their hemolymph has been widely used in medical applications for endotoxin detection. Nevertheless, there is limited information on the profiles of their hemolymph proteins and their gut microbial diversity. In this study, we performed the first detailed investigation of the hemolymph proteomics and gut microbiota of two Asian horseshoe crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda. Among the identified proteins being cataloged in the juvenile and adult hemolymph, unexpectedly, sesquiterpenoid signaling pathway proteins including Heat shock protein 83 (HSP83), Chd64, and a juvenile hormone binding protein (JHBP) were revealed. This provides evidence for the presence of functional sesquiterpenoid hormonal systems in these marine chelicerates. consumption of certain horseshoe crab species often leads to tetrodotoxin poisoning and the horseshoe crab is thought to possess a tetrodotoxin resistance mechanism. As such, sodium channels were analyzed and found to have critical amino acid residues that are similar to the toxin resistant pufferfish sodium channels. The source of the toxin is unknown so we investigated the gut microbiota, and found that Clostridium and Vibrio were the most dominant bacteria in T. tridentatus and C. rotundicauda, respectively. Together, this study provides a framework for further understanding of sesquiterpenoids and gut microbiota of these marine chelicerates.

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“…The balance of the intestinal environment affects the host’s growth and development, metabolic transformation, immune regulation, and many vital physiological functions ( Rawls et al, 2004 ; Flint et al, 2008 ; Butt and Volkoff, 2019 ). In recent years, Miao et al (2020) first studied the effects of initial feeding and first molting on the intestinal microbes of juvenile horseshoe crabs, who found that molting had a great impact on the intestinal microbes; Wang et al (2020) compared the differences of intestinal microbiota between Carcinoscorpius rotundicauda and T. tridentatus in Beibu Gulf. Qu et al (2020) analyzed the composition of the intestinal microbes of the tri-spine horseshoe crab in Hong Kong.…”

Section: Discussionmentioning

confidence: 99%

Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus

Liu

Xiong

et al. 2022

Front. Physiol.

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Anthropogenic elevation of atmospheric carbon dioxide (CO2) drives global-scale ocean acidification (OA), which has aroused widespread concern for marine ecosystem health. The tri-spine horseshoe crab (HSC) Tachypleus tridentatus has been facing the threat of population depletion for decades, and the effects of OA on the physiology and microbiology of its early life stage are unclear. In this study, the 1st instar HSC larvae were exposed to acidified seawater (pH 7.3, pH 8.1 as control) for 28 days to determine the effects of OA on their growth, molting, oxidative stress, and gut microbiota. Results showed that there were no significant differences in growth index and molting rate between OA group and control group, but the chitinase activity, β-NAGase activity, and ecdysone content in OA group were significantly lower than those of the control group. Compared to the control group, reactive oxygen species (ROS) and malondialdehyde (MDA) contents in OA group were significantly increased at the end of the experiment. Superoxide dismutase (SOD), catalase (CAT), and alkaline phosphatase (AKP) activities increased first and then decreased, glutathione peroxidase (GPX) decreased first and then increased, and GST activity changed little during the experiment. According to the result of 16S rRNA sequencing of gut microbiota, microbial-mediated functions predicted by PICRUSt showed that “Hematopoietic cell lineage,” “Endocytosis,” “Staphylococcus aureus infection,” and “Shigellosis” pathways significantly increased in OA group. The above results indicate that OA had no significant effect on growth index and molting rate but interfered with the activity of chitinolytic enzymes and ecdysone expression of juvenile horseshoe crabs, and caused oxidative stress. In addition, OA had adverse effects on the immune defense function and intestinal health. The present study reveals the potential threat of OA to T. tridentatus population and lays a foundation for the further study of the physiological adaptation mechanism of juvenile horseshoe crabs to environmental change.

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Intestinal Tract Microbe Communities Associated with Horseshoe Crabs from Beibu Gulf, China

Cited by 7 publications

References 36 publications

Identification of Lasso Peptide Topologies Using Native Nanoelectrospray Ionization-Trapped Ion Mobility Spectrometry–Mass Spectrometry

Identification of Lasso Peptide Topologies Using Native Nanoelectrospray Ionization-Trapped Ion Mobility Spectrometry–Mass Spectrometry

Hemolymph Proteomics and Gut Microbiota of Horseshoe Crabs Tachypleus tridentatus and Carcinoscorpius rotundicauda

Effects of Ocean Acidification on Molting, Oxidative Stress, and Gut Microbiota in Juvenile Horseshoe Crab Tachypleus tridentatus

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