Objective
To obtain normative data for the canine cornea and conjunctiva using high-resolution Time- and Fourier-Domain optical coherence tomography (TD-OCT and FD-OCT) and ultrasound pachymetry (USP).
Animals
One hundred sixty-eight eyes of 133 healthy, young, intact laboratory beagles.
Procedures
The cornea and conjunctiva of 16 eyes of 8 healthy young intact female intact beagles were imaged using FD-OCT. Corneal thickness was measured with FD-OCT and USP, while corneal and conjunctival epithelial thickness was measured with FD-OCT. The central corneal thickness (CCT) was determined in 152 eyes of 125 healthy young adult intact female (35) and male (90) beagles using TD-OCT. Mixed effects linear regression was used for statistical analysis.
Results
The CCT was (mean ± standard deviation) 497.54 ± 29.76, 555.49 ± 17.19 and 594.81 ± 33.02 μm as measured by FD-OCT, USP and TD-OCT, respectively. The central, superior paraxial, superior perilimbal corneal epithelial thickness and superior bulbar conjunctival epithelial thickness was 52.38 ± 7.27, 56.96 ± 6.47, 69.06 ± 8.84 and 42.98 ± 6.17 μm, respectively. When comparing techniques used for measuring CCT (USP versus FD-OCT and FD-OCT versus TD-OCT), USP and TD-OCT generated significantly greater values in comparison to FD-OCT (both P < 0.001). For all dogs CCT increased with increasing age and body weight (both P < 0.001) and was higher in intact males versus females using TD-OCT (P = 0.034).
Conclusion
High-resolution FD-OCT and TD-OCT provide detailed non-invasive evaluation of in vivo canine anterior segment structures. Normative values of the canine cornea and conjunctiva are reported.