Intra-and interspecific protoplast fusion between some Pleurotus species.

Toyomasu, Tetsuo; Mori, Kanichi

doi:10.1271/bbb1961.51.935

Cited by 6 publications

(4 citation statements)

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“…Mycelial growth shown by mycelial colony diameter and hyphal size of FS1 and FS2 was significantly different from those of the parental strains (Table 1). These results are consistent to the previous studies indicating that fusants which are dikaryotic (n+n) grow faster (Toyomatsu and Mori 1987) and have larger hyphae (Abe et al 1982) than the monokaryotic parental strains. There were 10 replicates of mycelia taken from three different colonies of FS1 and FS2 fusants as well as their parents used in this experiment.…”

Section: Mycelial Growth and Hyphal Sizesupporting

confidence: 93%

“…Ira Djajanegara a,b and Agus Masduki a a Agency for the Assessment andApplication of Technology LAPTIAB Gedung 610, PUSPIPTEK Serpong 15314, Indonesia, email: idjajanegara@yahoo.com/agusmasduki2003@yahoo.com b University of Al Azhar Indonesia Kompleks Masjid Al Azhar, Jalan Sisingamangaraja No. 8, Kebayoran Baru, Jakarta Submitted 6 July 2009;Accepted 7 January 20101993Matsumoto et al 1997), intergenerically (Eguchi et al 1993;Zhao and Chang 1996) and even interheterogenerically (Eguchi and Higaki 1995;Toyomatsu and Mori 1987). However, for the same reason as that implied to the conventional method, the greater the distance in genetic relationship between the two mating isolates, the less successful protoplast fusion will be (Anne and Peberdy 1976).…”

Section: Protoplast Fusion Between White and Brown Oyster Mushroomsmentioning

confidence: 99%

“…Protoplast fusion has been used as a method to create mushroom hybrids especially when conventional method cannot be achieved. As conventional hybridization, protoplast fusion can be performed intraspecifically (Kiguchi and Yanagi 1985;Toyomatsu and Mori 1987), interspecifically (Takehara et al…”

Section: Introductionmentioning

confidence: 99%

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Protoplast Fusion Between White and Brown Oyster Mushrooms

Djajanegara

Masduki

2013

Indones. J. Agric. Sci.

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Genetic crossing of white oyster mushroom (Pleurotus floridae) to introduce longer storage life trait can only be done within individuals in this particular species. However, longer storage life trait is possessed by brown oyster mushroom (Pleurotus cystidiosus). Therefore, a protoplast fusion experiment between white and brown oyster mushrooms was conducted to obtain an oyster mushroom strain showing high productivity and long storage life. The experiment was done at the biology laboratory of the University of Al Azhar Indonesia from May 2008 to August 2009. Protoplast fusion was done by isolating protoplast from 5-day old monokaryotic mycelia grown in potato dextrose broth (PDB). Around 3.15 x 105 protoplasts ml-1 were harvested using mixture of cellulase Onozuka R-10 (1%) and macerozyme R-10 (1%) from brown oyster mushroom with 80.61% viability. Similarly, 3.71 x 105 protoplasts ml-1 were harvested using lysing enzyme (2%) from white oyster mushroom with 83.68% viability. Protoplast fusions were conducted using 40% PEG6000 for 10 minutes. The candidate fusants were then screened using minimum regeneration media (MRM). There were 22 colonies grew on MRM media and four colonies (FS1, FS2, FS3, and FS4) showed clamp connection as well as primordia formation to be chosen as candidate fusants. However, isozyme studies using malate dehydrogenase and acid phosphatase as marker enzymes confirmed that only FS1 and FS2 were the hybridized products. The two colonies showed different mycelia growth patterns and hyphae sizes, fruit body morphology and productivity compared to their parents. These two fusants, however, did not indicate the presence of longer storage life trait as expected despite a higher productivity achieved by FS1. In this study, the protoplast fusion only yielded higher productivity strain of mushroom with different colors without any changes in storage life.

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Section: Mycelial Growth and Hyphal Sizesupporting

confidence: 93%

Section: Protoplast Fusion Between White and Brown Oyster Mushroomsmentioning

confidence: 99%

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Protoplast Fusion Between White and Brown Oyster Mushrooms

Djajanegara

Masduki

2013

Indones. J. Agric. Sci.

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“…MnP gene cDNA is sequenced from P. florida (8) and A. bisporus (9). Hybrid production by fusion of monokaryotic and dikaryotic of Pleurotus species has been presented (10). The hybrids Pleurotus ostreatus (11) and Lentinula edodes (12) were generated using hyphae anastomosis fusion.…”

Section: Introductionmentioning

confidence: 99%

Production of Somatic Hybrids of Pleurotus florida Through Hyphae Anastomosis Fusion with Agaricus bisporus

Farimani

Farsi

2022

Gene Cell Tissue

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Background: Production and cultivation of edible mushrooms are one of the commercial applications of microbial technologies in order to biodegrad agricultural waste into valuable food products. One of the edible mushrooms that uses these agricultural wastes for its growth and development is Pleurotus florida. In P. florida, various enzymes are responsible for the decomposition of lignin compounds in the compost environment from the beginning of mycelial growth to the end of the fruiting period. One of the most important of these enzymes is manganese peroxidase (MnP), which plays a major role in the breakdown of lignin compounds. In addition, the most important compost-degrading mushroom that produces the enzyme MnP is Agaricus bisporus. Objectives: The aim of this study was to produce hybrids of P. florida by increasing the production efficiency through hyphae fusion of P. florida and the A. bisporus. Methods: The hyphae fusion and hybridization were performed for two strains P. florida and A. bisporus, in PDA culture media. Samples were isolated from the fusion areas and were transferred to the culture media, and then spawn was produced. Performance testing was carried out with hybrids and P. florida. We designed a specific primer pair of P. florida MnP gene by Primer Premier software (V.7.0). Polymerase chain reaction (PCR) was used to confirm the MnP gene. For this purpose, RNAs were extracted, and their cDNA was synthesized by reverse transcriptase enzyme. PCR with specific primers was performed, and electrophoresis was loaded on 1% agarose gel. Results: This study performed hybridization with formation clamp connections at the junction line. Hybrid N09 with biological efficiency (82.92) produced the highest performance in all evaluations compared to other hybrids and the parent strain. Based on the results of PCR product electrophoresis, banding pattern N09 (hybrid N09) indicated that this hybrid, in addition to the P. florida MnP gene of P. florida, also received the amino acid sequences of this gene in A. bisporus. Conclusions: According to the obtained results, the use of hyphae fusion increased the traits as a result of mixing the genetic contributions of parent strains, followed by the diversity of hybrid strains and increased production yield.

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Mushroom Genetics and Breeding

Wu¹

1990

Plant Breeding Reviews

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Intra-and interspecific protoplast fusion between some Pleurotus species.

Cited by 6 publications

References 0 publications

Protoplast Fusion Between White and Brown Oyster Mushrooms

Protoplast Fusion Between White and Brown Oyster Mushrooms

Production of Somatic Hybrids of Pleurotus florida Through Hyphae Anastomosis Fusion with Agaricus bisporus

Mushroom Genetics and Breeding

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