2002
DOI: 10.1017/s0953756202006032
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Intra- and intersporal diversity of ITS rDNA sequences in Glomus intraradices assessed by cloning and sequencing, and by SSCP analysis

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Cited by 63 publications
(46 citation statements)
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“…We were surprised to find similar levels of AM fungal diversity in natural areas and adjacent croplands of the prairie. Numerous studies have reported negative effects of crop production on AM fungi (52)(53)(54)(55). Soil tillage has a negative impact on AM hyphal network biomass and infectivity (56,57) and on the richness of AM fungal communities (58)(59)(60), but in the Canadian prairie, soils are rarely tilled (25), as not tilling is a soil water conservation practice (61,62).…”
Section: Discussionmentioning
confidence: 99%
“…We were surprised to find similar levels of AM fungal diversity in natural areas and adjacent croplands of the prairie. Numerous studies have reported negative effects of crop production on AM fungi (52)(53)(54)(55). Soil tillage has a negative impact on AM hyphal network biomass and infectivity (56,57) and on the richness of AM fungal communities (58)(59)(60), but in the Canadian prairie, soils are rarely tilled (25), as not tilling is a soil water conservation practice (61,62).…”
Section: Discussionmentioning
confidence: 99%
“…Single-spore isolates were then maintained in identical environments ( Fig. 1) (24). Four single-spore isolates were taken from each of the four plots and put into culture with Root tumor-inducing plasmid T-DNA-transformed carrot roots, which were all of the same clone (25) (for a detailed description of isolation and cultivation see Supporting Text, which is published as supporting information on the PNAS web site).…”
Section: Methodsmentioning
confidence: 99%
“…However, identification of AMF on the species or even isolate level is complicated by the heterogeneity of rDNA within glomeromycotan spores and isolates. Several authors have shown that different variants of rDNA genes coexist within single AMF spores (15,27). Hence, a conservative approach in the evaluation of phylogenetic analyses is advisable by, e.g., defining a well-supported monophyletic sequence cluster as an AMF phylotype.…”
mentioning
confidence: 99%