Intra-articular Injection of Urinary Bladder Matrix Reduces Osteoarthritis Development

Jacobs, Heather; Rathod, Sona; Wolf, Matthew T.; Elisseeff, Jennifer H.

doi:10.1208/s12248-016-9999-6

Cited by 20 publications

(10 citation statements)

References 36 publications

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“…muscle repair, IL-4 production by Th2 cells was required for the myeloid cell transition to a proregenerative phenotype, needed for efficient muscle repair (102). In the joint, IL-4 production correlated with tissue repair after ACLT, suggesting that similar mechanisms of type 2 immune responses impact OA (103). Without treatment, OA signatures reflect a profibrotic and proinflammatory immune response that is potentially mediated by SnCs, which may only recruit and modulate the phenotypes of immune cells.…”

Section: R E V I E W S E R I E S : C E L L U L a R S E N E S C E N C mentioning

confidence: 99%

Senescent cells and osteoarthritis: a painful connection

Jeon¹,

David²,

Campisi³

et al. 2018

Journal of Clinical Investigation

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254

217

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Senescent cells (SnCs) are associated with age-related pathologies. Osteoarthritis is a chronic disease characterized by pain, loss of cartilage, and joint inflammation, and its incidence increases with age. For years, the presence of SnCs in cartilage isolated from patients undergoing total knee artificial implants has been noted, but these cells' relevance to disease was unclear. In this Review, we summarize current knowledge of SnCs in the multiple tissues that constitute the articular joint. New evidence for the causative role of SnCs in the development of posttraumatic and age-related arthritis is reviewed along with the therapeutic benefit of SnC clearance. As part of their senescence-associated secretory phenotype, SnCs secrete cytokines that impact the immune system and its response to joint tissue trauma. We present concepts of the immune response to tissue trauma as well as the interactions with SnCs and the local tissue environment. Finally, we discuss therapeutic implications of targeting SnCs in treating osteoarthritis.

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Section: R E V I E W S E R I E S : C E L L U L a R S E N E S C E N C mentioning

confidence: 99%

Senescent cells and osteoarthritis: a painful connection

Jeon¹,

David²,

Campisi³

et al. 2018

Journal of Clinical Investigation

Self Cite

254

217

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“…Besides joint damage, MIA injection induces mechanical sensitivity of the ipsilateral hind paw and weight bearing deficit. Histological observation and pain-related behavior in rat models together resemble human degenerative OA (Jacobs et al 2017).…”

Section: Introductionmentioning

confidence: 87%

“…Osteoarthritis (OA) is the most noticeable form of the synovial joint disease, characterized by joint degeneration and pain. In addition to structural defects, there is increasing evidence that approximately 30% of OA patients have neuropathic pain (Teeple et al 2013;Wang and Regatte 2015;Rey-Rico et al 2016;Jacobs et al 2017;Jin et al 2017;Philpott et al 2017). The mechanisms of persistent pain development are not yet clear, but most of modern OA treatment strategies are based on pain management (Ma et al 2009;Van Velden et al 2015;Philpott et al 2017;Zhang et al 2017;Kalamegam et al 2018).…”

Section: Introductionmentioning

confidence: 99%

A novel view of the problem of Osteoarthritis in experimental rat model

Паликов¹,

Palikova²,

Borozdina³

et al. 2020

RRP

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Introduction: The article presents the results of the functional tests to improve the assessment of MIA-induced osteoarthritis development and the effectiveness of NSAID therapy. Materials and methods: In the study, 26 male SD rats were used. MIA-induced osteoarthritis was simulated in the right knee joint. After an intra-articular injection of MIA, the animals were treated with ibuprofen and meloxicam. Pain assessment was studied in the following functional tests: incapacitance (hind limb weight bearing) test, von Frey test (mechanical allodynia), grip strength test, and knee diameter measurement. At the end of the study, a histological analysis of the knee joint was performed. Results and discussion: An intra-articular MIA injection reduced 1.5 times the paw withdrawal threshold. In the rats that suffered MIA-induced osteoarthritis, the difference between the diameters of the intact and injected joints was 1.05 mm, compared to 0.03 mm difference in the control group. Hind limb weight bearing asymmetry was 89.5% when simulating MIA-induced osteoarthritis. The muscular hind limb grip strength in rats with MIA-induced osteoarthritis was significantly reduced on 3rd and 7th days after simulating osteoarthritis. Ibuprofen and meloxicam showed significant efficacy in all the above tests, although ibuprofen effectiveness was more pronounced than that of meloxicam. Conclusion: The following functional tests were identified as the most significant and sufficient to assess the development of MIA-induced osteoarthritis and analgesic efficacy of NSAIDs: incapacitance test, allodynia test (von Frey filaments), measurement of hind limb grip strength and measurement of the diameter of the inflamed knee joint. The histological analysis made it possible to confirm the correspondence of the physiological response and pathological changes in the knee joint.

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“…It has been demonstrated that the presence and integrity of basement membrane complex in UBM promotes inductive tissue remodeling (Brown and Badylak, 2014 ), but little is known about the supporting activity of UBM toward chondrocyte function. UBM was recently used for articular cartilage regeneration in canine and murine models demonstrating its efficacy in treating dogs or mice with chronic osteoarthritis of the hip or knee joint, respectively (Rose et al, 2009 ; Tottey et al, 2011 ; Jacobs et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair

Angelozzi

Penolazzi

Mazzitelli

et al. 2017

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) approaches using biomaterials have gain important roles in the regeneration of cartilage. This paper describes the production by microfluidics of alginate-based microfibers containing both extracellular matrix (ECM)-derived biomaterials and chondrocytes. As ECM components gelatin or decellularized urinary bladder matrix (UBM) were investigated. The effectiveness of the composite microfibers has been tested to modulate the behavior and redifferentiation of dedifferentiated chondrocytes. The complete redifferentiation, at the single-cell level, of the chondrocytes, without cell aggregate formation, was observed after 14 days of cell culture. Specific chondrogenic markers and high cellular secretory activity was observed in embedded cells. Notably, no sign of collagen type 10 deposition was determined. The obtained data suggest that dedifferentiated chondrocytes regain a functional chondrocyte phenotype when embedded in appropriate 3D scaffold based on alginate plus gelatin or UBM. The proposed scaffolds are indeed valuable to form a cellular microenvironment mimicking the in vivo ECM, opening the way to their use in cartilage TE.

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Intra-articular Injection of Urinary Bladder Matrix Reduces Osteoarthritis Development

Cited by 20 publications

References 36 publications

Senescent cells and osteoarthritis: a painful connection

Senescent cells and osteoarthritis: a painful connection

A novel view of the problem of Osteoarthritis in experimental rat model

Dedifferentiated Chondrocytes in Composite Microfibers As Tool for Cartilage Repair

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