Intra-species genomic groups in Fusarium semitectum and their correlation with origin and cultural characteristics

Abd‐Elsalam, Kamel A.; Schnieder, F.; Asran-Amal, A.; Khalil, Mohamed S.; Verreet, Joseph‐Alexander

doi:10.1007/bf03356118

Cited by 12 publications

(13 citation statements)

References 20 publications

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“…A positive correlation between the genetic diversity of isolates and the geographical distance has been reported for Gibberella zeae in the USA (Zeller et al., 2004), Sclerospora graminicola in India (Singru et al., 2003) and Alternaria brassicicola in New South Wales (Bock et al., 2002). For isolates of Beauveria bassiana from Kenya (De Muro et al., 2003), Fusarium semitectum on cotton in Egypt (Abd‐Elsalam et al., 2003) and Peronospora sparsa in Finland (Lindqvist‐Kreuze et al., 2002), however, no correlation between physical distance of origin and genetic diversity could be established.…”

Section: Discussionmentioning

confidence: 99%

“…To study the molecular diversity of Ethiopian and German P. triticina isolates, amplified fragment length polymorphism (AFLP) analysis was chosen because AFLPs (i) generate more information per analysis than other molecular techniques, (ii) require no prior knowledge about the genetic make‐up of the organism under study, (iii) are highly reproducible, and (iv) allow discrimination among closely related individuals within a species (Vos et al., 1995; Majer et al., 1996; Mueller and Wolfenbarger, 1999). AFLP markers have been successfully used in mycology and plant pathology for the differentiation of species within genera (Keiper et al., 2003; Menzies et al., 2003; Schmidt et al., 2004; Leisova et al., 2005b) as well as of isolates within species from straminopiles, ascomycetes and basidiomycetes (Schnieder et al., 2001; Rau et al., 2002, 2005; Abd‐Elsalam et al., 2003; Radisek et al., 2003; Singru et al., 2003; Martinez et al., 2004; Leisova et al., 2005a,b; Kauserud et al., 2006). Molecular techniques such as AFLP produce neutral markers in contrast to resistance‐specific virulence, which is subject to strong host selection (Kolmer, 1993; Brown, 1996) and environmental conditions.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Diversity in Puccinia triticina Isolates from Ethiopia and Germany

Mebrate¹,

Dehne²,

Pillen

et al. 2006

Journal of Phytopathology

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A total of 43 isolates of the wheat leaf rust fungus, Puccinia triticina Eriks, collected from Ethiopia and Germany were analysed for their genetic diversity using the amplified fragment length polymorphism (AFLP) technique. Out of 18 EcoRI/MseI primer combinations screened, 15 produced 219 highly polymorphic fragments. The average AFLP difference between pairs of the leaf rust isolates (26 from Ethiopia, 17 from Germany) was calculated using Dice's genetic similarity (GS) coefficient. The overall GS for the 43 isolates was 0.67 ± 0.13. The Ethiopian leaf rust isolates had lower average GS (0.63 ± 0.13) than the German ones (0.76 ± 0.10). A cluster analysis and a two-dimensional principal coordinate analysis (PCoA) grouped the 43 isolates into two significantly different (P £ 0.01) clusters. Isolates in cluster I (35 isolates) had an average GS of 0.76 ± 0.06 while the isolates in cluster II (8 isolates) had an average GS of 0.55 ± 0.12. Isolates were also grouped into three regions of collection, central Ethiopia, south and south-east Ethiopia, and Germany. The regions were significantly different at P £ 0.01 indicating regional variation in terms of molecular diversity of the leaf rust isolates studied. Each isolate, however, had a unique AFLP fingerprint. The results indicated that the leaf rust population in central Ethiopia is genetically distinct and this might be related to the predominant cultivation of durum wheat cultivars in this area.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Diversity in Puccinia triticina Isolates from Ethiopia and Germany

Mebrate¹,

Dehne²,

Pillen

et al. 2006

Journal of Phytopathology

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“…Different authors reported the lack of correlation between physical distance of origin and genetic diversity in other fungus genus. Abd‐Elsalam et al. (2003) working with Fusarium semitectum did not find association between the genetic variability and the host genotype or the geographic origin.…”

Section: Discussionmentioning

confidence: 83%

Molecular Diversity of Puccinia melanocephala Populations

Pocoví

Rech

Collavino

et al. 2010

Journal of Phytopathology

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AFLP analysis was carried out to assess genetic variability and determine the population structure of the sugarcane rust Puccinia melanocephala in northwest Argentina. Molecular data were also used to clarify whether genetic variation was correlated with host variation and ⁄ or the geographic distribution of the disease. Bulk rust uredospores were collected in the field, and both the geographical area and the infected host sugarcane cultivar were recorded. A total of 538 AFLP markers generated with 20 primer combinations were used to perform the genetic analysis. The percentage of polymorphic loci was quite high (85.7%), considering that P. melanocephala only reproduces asexually. Cluster analysis (UPGMA) and principal co-ordinate analysis (PCoA) grouped populations from distinct geographic and host origins, suggesting that neither geographical region nor sugarcane variety constrains the relationships among the populations. This finding was corroborated by a lack of significant correlation between genetic distance and geographic distance (r = 0.057; P = 0.285). The non-significant differences found between rust populations collected from distinct sugarcane varieties (PhiT = 0.026; P = 0.06) also support these results. Analysis of Molecular Variance Approach (AMOVA) analysis attributed most of the variation (95%) to differences within populations. No genetic structure was detected, and the populations behaved as a large undifferentiated population with high level of genetic variability.

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“…High levels of intraspecific variability exist within F. semitectum isolates that are obtained from a single host, such as cotton (ABD-ELSALAM et al, 2003), alfalfa (ZACCARDELLI et al, 2006) and red-fleshed dragon fruit (HAWA et al, 2010). In our case, the occurrence F. semitectum isolates with similar virulence levels in melon fruits may be due to the low number of isolates because they all came from the same agricultural region.…”

Section: Resultsmentioning

confidence: 98%

<b>Effects of wounding, humidity, temperature, and inoculum concentrations on the severity of corky dry rot caused by <i>Fusarium semitectum</i> in melon fruits

et al. 2014

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Corky dry rot, caused by Fusarium semitectum, is the main postharvest disease of melons in Brazil. This study investigated the effects of wounding, humidity, temperature, and inoculum concentration on the severity of corky dry rot under controlled conditions. Cantaloupe and honeydew melon types were inoculated by spraying conidial suspensions of three F. semitectum isolates. In all experiments, the tested F. semitectum isolates did not differ in relation to disease severity, but, the cantaloupe melon showed higher levels of severity. No lesions appeared on fruits that lacked wounds, and increasing wound age reduced lesion severity. Melons that were inoculated with F. semitectum developed symptoms regardless of the presence or absence of a moist chamber at the post-inoculation stage, but the lesions were larger under moist chamber conditions. There were no symptoms at 10°C, but a temperature increase from 15 to 25°C resulted in a disease severity increase. The largest lesions were observed when both melon types were inoculated with a concentration of 10 6 conidia mL -1 , but even the lowest concentration (10 1 conidia mL -1 ) was sufficient for causing lesions. Injury reduction and/or the acceleration of melon healing, as well as environmental variable control and a reduction of inoculum sources, are essential to reducing corky dry rot severity.Efeito de ferimento, umidade, temperatura e concentração de inóculo na severidade da podridão seca causada por Fusarium semitectum em frutos de melão RESUMO. A podridão seca causada por Fusarium semitectum é a principal doença pós-colheita do melão no Brasil. Este estudo investigou o efeito de ferimento, umidade, temperatura e concentração de inóculo na severidade da podridão seca em condições controladas. Frutos de melão dos tipos cantaloupe e honeydew foram inoculados por meio da pulverização de suspensão de conídios de três isolados de F. semitectum, inoculados separadamente. Em todos os experimentos os isolados de F. semitectum testados não diferiram em relação à severidade da doença, porém, a variedade cantaloupe apresentou maiores níveis de severidade da doença. Não houve lesões em frutos sem ferimento e o incremento na idade do ferimento reduziu a severidade. Melões inoculados com F. semitectum desenvolveram sintomas independentemente de câmara úmida pós-inoculação, mas as lesões foram maiores em condições de câmara úmida. Não houve sintomas a 10°C, mas o aumento da temperatura de 15 para 25°C resultou num incremento da severidade da doença. As maiores lesões foram observadas na concentração de inóculo de 10 6 conídios mL -1 nos dois tipos de melão, mas a menor concentração (10 1 conídios mL -1 ) foi suficiente para causar leões. A diminuição de injúrias nos frutos de melão e/ou a aceleração da cicatrização, bem como o controle das variáveis ambientais e a redução das fontes de inóculo são essenciais para redução da severidade da podridão seca.Palavras-chave: Cucumis melo, podridão do fruto, patologia pós-colheita, epidemiologia. 282Oliveira et al.

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Intra-species genomic groups in Fusarium semitectum and their correlation with origin and cultural characteristics

Cited by 12 publications

References 20 publications

Molecular Diversity in Puccinia triticina Isolates from Ethiopia and Germany

Molecular Diversity in Puccinia triticina Isolates from Ethiopia and Germany

Molecular Diversity of Puccinia melanocephala Populations

<b>Effects of wounding, humidity, temperature, and inoculum concentrations on the severity of corky dry rot caused by <i>Fusarium semitectum</i> in melon fruits

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