Intraarticular application of autologous conditioned serum (ACS) reduces bone tunnel widening after ACL reconstructive surgery in a randomized controlled trial

Daraboš, Nikica; Hašpl, Miroslav; Moser, Carsten; Daraboš, Anela; Bartolek, Dubravka; Groenemeyer, Dietrich H.W.

doi:10.1007/s00167-011-1458-4

Cited by 89 publications

(55 citation statements)

References 78 publications

(110 reference statements)

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“…The number of patients required for this second part of the study (double-bundle) was re-estimated on the basis of the effect sizes (ca. 0.5) observed in the first part on the single-bundle treatment [7], using a significance level alpha of 0.05 and a test power of 0.5. The expectation was a sample size of 31 patients in each study group.…”

Section: Discussionmentioning

confidence: 99%

“…It provides elevated but physiological concentrations of autologous IL-1Ra and other important signalling proteins [45; Table 5]. ACS has previously been demonstrated to reduce bone tunnel widening in SB ACL reconstruction [7], to augment tendon healing [26] and muscle healing [47,48], and has proved useful in the treatment of knee osteoarthritis [1] and radicular inflammation [2]. Faster healing after surgery means less muscle loss and earlier return to full rehabilitation and training.…”

Section: Discussionmentioning

confidence: 99%

“…intra-articular injections of autologous conditioned serum (ACS) reduce bone tunnel widening in patients undergoing SB ACL reconstruction [7]. Severe bone tunnel widening may be one reason for joint laxity and increased failure rates of ACL reconstruction.…”

mentioning

confidence: 99%

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RETRACTED ARTICLE: Comparison of double-bundle anterior cruciate ligament reconstruction with and without autologous conditioned serum application

Daraboš

Tršek²,

Miklić

et al. 2014

Knee Surg Sports Traumatol Arthrosc

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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RETRACTED ARTICLE: Comparison of double-bundle anterior cruciate ligament reconstruction with and without autologous conditioned serum application

Daraboš

Tršek²,

Miklić

et al. 2014

Knee Surg Sports Traumatol Arthrosc

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“…203,205 In addition to application in the treatment of OA, ACS has also shown to be effective in the treatment of sciatic pain 206 , tendon healing 207 , and to reduce bone tunnel widening following anterior cruciate ligament surgery. 208 ACS has also been shown to enhance muscle healing in both experimentally in mice and in a clinical setting. stifles.…”

Section: Autologous Conditioned Serummentioning

confidence: 99%

Cytokine and Growth Factor Concentrations in Canine Autologous Conditioned Serum

et al. 2016

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(ACADEMIC)The object of this study was to compare growth factor and cytokine profiles in canine autologous conditioned serum (ACS) to canine plasma. Blood collected from 16 medium to large breed dogs was used to produce ACS (Orthokine ® vet irap 10 syringes) and citrated plasma (control). Canine-specific ELISA assays were run per manufacturers' instructions for interleukin (IL)-10, IL-4, tumor necrosis factor (TNF)-α, insulin-like growth factor (IGF)-1, fibroblast growth factor (FGF)-2, transforming growth factor (TGF)-β1, IL-1β, and interleukin-1 receptor antagonist (IL-1ra). Serum, in addition to plasma and ACS, was collected from an additional 6 dogs for TNF-α, IL-1β, and IL-1ra analysis (total of 22 dogs). Data were analyzed for differences in cytokine concentrations between ACS, plasma, and serum using the Wilcoxon signed-rank test with significance set at P<.05.There was a large variability in growth factor and cytokine concentrations between individual dogs in both plasma and ACS. There were no significant differences in IL-10, TNF-α, IGF-1, FGF-2, and TGF-β1 concentrations between ACS, plasma, or serum. ACS concentrations of IL-1β (median, range; 46.3 pg/mL, 0-828.8) and IL-4 (0.0 pg/mL, 0-244.1) were significantly increased compared to plasma (36.6 pg/mL, 0-657.1 and 0.0 pg/mL, 0-0, respectively). IL-1ra concentrations in ACS (median, range; 3458.9 pg/mL, 1,243.1-12,089.0) were significantly higher than plasma (692.3 pg/mL, 422.5-1,475.6), as was the IL-1ra:IL-1β ratio (39.9 and 7.2, respectively).iii Cytokine and Growth Factor Profiles in Canine Autologous Conditioned SerumDominique Marie Sawyere ABSTRACT (PUBLIC)Osteoarthritis (OA) is a common cause of pain and suffering in dogs and is challenging to treat effectively Current treatment for OA merely mask the signs of pain and do not promote regeneration of the damaged cartilage or alter the course of the disease. Many treatments also cause harmful side effects. Autologous conditioned serum (ACS) is a biological therapy that controls inflammation within the joint by specifically blocking the inflammatory cytokine interleukin (IL)-1β. In humans and horses ACS relieves the pain from OA and promotes cartilage regeneration. The purpose of this study was to compare levels of pro-and anti-inflammatory cytokines in canine ACS to that of normal canine plasma with the hypothesis that levels of anti-inflammatory cytokines would be higher in ACS compared to controls and that levels of pro-inflammatory cytokines would remain unchanged. Paired blood samples were collected from 22 large breed healthy dogs and processed in either irap®10 syringes (ACS) or in anti-coagulant as a control. For the last 6 dogs an additional serum sample was collected. Growth factor and cytokine levels were determined using canine-specific ELISAs. One pro-inflammatory cytokine (IL-1β) and two anti-inflammatory cytokines (IL-1ra and IL-4) were significantly higher in the ACS compared to plasma and IL-1ra levels were also higher in serum compared with plasma samples. Other levels w...

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“…Biomarcadores são medidas bioquímicas, indicativas de um processo biológico ou de resposta à uma intervenção (STEEL, 2008 (POOLE et al, 1994;MURAKAMI et al, 1998;SAITO et al, 2002 (DARABOS et al, 2009;DARABOS et al 2011).…”

Section: O Estresse Oxidativo E O Líquido Sinovialunclassified

Investigação laboratorial dos efeitos antioxidantes do plasma processado autólogo nas principais enfermidades articulares de equinos após tratamento artroscópico

Brossi¹

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Palavras-chave:Equino. Líquido sinovial. Hemoderivado. Estresse oxidativo. Plasma processado autólogo. As a cursorial animal, the horse relies on soundness to fully accomplish daily life activities and survival. Horses' natural ability for movement justifies breeding and training investments, and is explored to its maximum during athletic activities. Articular disorders, such as osteoarthritis and osteochondrosis, with their potential to reduce physical performance, are a therapeutic challenge. Autologous haemoderivates have appeared as safe and accessible options for the management of these conditions, but their widespread use has outpaced scientific evidence regarding mechanisms of action and ideal methods for preparation and administration. The antioxidant properties of autologous processed plasma, previously demonstrated in vitro on stimulated equine synovial fluid cells, posed a question on whether these effects would occur in vivo. Therefore, the present study aimed to verify autologous processed plasma´s effects in osteoarthritic and osteochondritic joints, when administered at the end of the arthroscopic procedure, with particular emphasis on possible antioxidant attributes. The study also aimed to detect evidences of oxidative stress in these disorders, what could further justify its use. For this purpose, synovial fluid was obtained immediately before the beginning of the surgery, and analyzed to yield an oxidant/ antioxidant, physical, cellular, molecular and inflammatory profile of osteoarthritis and osteochondrosis. After the end of the procedure, one group of horses received the intraarticular medication and another group of untreated horses served as controls. Forty-eight hours after surgery synovial fluid was retrieved from operated joints, and a second analysis was performed. Physical and cellular analyses were conducted immediately after sample collection and the supernatant was stored at -80º C for further evaluations. These consisted in catalase, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, vitamin E, protein carbonyl groups, chondroitin sulphate, hyaluronic acid, prostaglandin E 2 , total protein and urea determinations.Osteoarthritic joints revealed greater activation of antioxidant defense systems, enzymatic and non-enzymatic, with greater activity of catalase, and greater concentrations of vitamin E, respectively, when compared to osteochondritic joints. Additionally, these joints had higher total nucleated cell counts and prostaglandin E 2 and total protein concentrations, revealing a more inflammatory profile when compared to that of osteochondritic joints. Nonetheless, in osteochondritic joints synovial fluid and matrix components degradation was more pronounced, as there were higher concentrations of chondroitin sulphate and hyaluronic acid in synovial fluid obtained from these joints. Results also showed the occurrence of oxidative stress, 48 hours after the arthroscopic procedure, through higher catalase activity in synovial fluid, reflecting activation ...

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Intraarticular application of autologous conditioned serum (ACS) reduces bone tunnel widening after ACL reconstructive surgery in a randomized controlled trial

Abstract: The intraarticular administration/injection of ACS results in decreased bone tunnel widening after ACL reconstructive surgery.

Cited by 89 publications

References 78 publications

RETRACTED ARTICLE: Comparison of double-bundle anterior cruciate ligament reconstruction with and without autologous conditioned serum application

RETRACTED ARTICLE: Comparison of double-bundle anterior cruciate ligament reconstruction with and without autologous conditioned serum application

Cytokine and Growth Factor Concentrations in Canine Autologous Conditioned Serum

Investigação laboratorial dos efeitos antioxidantes do plasma processado autólogo nas principais enfermidades articulares de equinos após tratamento artroscópico

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