Intracellular calcium and protein tyrosine phosphorylation during the release of bovine sperm adhering to the fallopian tube epithelium in vitro

Gualtieri, Roberto; Boni, Raffaele; Tosti, Elisabetta; Zagami, Maria; Talevi, Riccardo

doi:10.1530/rep.1.00374

Cited by 63 publications

(47 citation statements)

References 35 publications

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“…However, in the present study, we observed that AEA has no effects on sperm-oviduct binding and sperm release, even at a concentration of 5 mM. These data are in agreement with several evidences showing that release of spermatozoa bound to the oviductal reservoir is promoted by capacitation (Lefebvre & Suarez 1996, Gualtieri et al 2005, Talevi et al 2007, Talevi & Gualtieri 2010, whereas AEA has been shown to depress this process, at least in human and boar (Rossato et al 2005, Maccarrone et al 2005. Herein, sperm-ZP binding experiments demonstrated that AEA depresses sperm capacitation also in the bovine species.…”

Section: Sperm-oviduct Interactionsupporting

confidence: 93%

“…Several studies showed that sperm adhesion to the oviductal epithelium prevents capacitation and depresses the motility (reviewed in Hunter (2008)). However, at the time of release, adhering spermatozoa undergo rapid metabolic changes such as increase in intracellular Ca 2C , protein tyrosine phosphorylation, and flagellar beat frequency, which allow their migration toward the ampullary region (Gualtieri et al 2005). The sperm ascension through the oviduct may involve intermittent phases of adhesions and releases (Smith & Yanagimachi 1990, Demott & Suarez 1992, Nakanishi et al 2004.…”

Section: Introductionmentioning

confidence: 99%

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Is there a role for endocannabinoids in sperm–oviduct interaction?

Talevi¹,

Barbato²,

Iorio³

et al. 2010

Reproduction

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The endocannabinoid system (ECS) has been found in reproductive cells and tissues in several mammals. Spermatozoa are able to respond to anandamide, and the oviduct is able to synthesize and modulate the concentration of this endocannabinoid along the isthmic and ampullary regions. The main aim of this study was to understand whether the ECS has a role during sperm storage and release within the oviduct in cattle. Data showed that 1) the endocannabinoid receptors 1 and 2 (CB1 and CB2) are present in bovine spermatozoa both in the initial ejaculate and in spermatozoa bound to the oviduct in vitro; 2) CB1 receptor is still detectable in spermatozoa released from the oviduct through penicillamine but not in those released through heparin; 3) arachidonylethanolamide (AEA) does not affect sperm viability, whereas it depresses sperm progressive motility and kinetic values; 4) sperm-oviduct binding and release in vitro are not influenced by AEA; 5) AEA depresses sperm-zona pellucida (ZP) binding; 6) binding of heparin-capacitated spermatozoa to the ZP is not affected by AEA; 7) N-acylphosphatidylethanolamine-selective phospholipase D, the main enzyme involved in anandamide synthesis, is expressed in oviductal epithelial cells. In conclusion, secretion of AEA from epithelial cells might contribute to the oviduct sperm-reservoir function, prolonging the sperm fertile life through the depression of motility and capacitation. Capacitation signals, such as heparin, that promote sperm release, might remodel the sperm surface and cause a loss of the sperm sensitivity to AEA.

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Section: Sperm-oviduct Interactionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Is there a role for endocannabinoids in sperm–oviduct interaction?

Talevi¹,

Barbato²,

Iorio³

et al. 2010

Reproduction

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“…There is also evidence from in vitro studies that sulphated glycoconjugates such as heparin can act on oviduct tissues to release sperm from binding (Talevi and Gualtieri, 2001;Gualtieri and Talevi, 2003;Gualtieri et al, 2005). However, very much in line with the overall theme of this essay, the concentrations of heparin or heparin-like glycoconjugates in oviduct luminal fluid are under cyclic ovarian control, reaching a peak during the period of oestrus (Parrish et al, 1989), so one returns to the perspective of gonadal coordination of pre-fertilisation events.…”

Section: Catecholamine and Ca 2þ Ionmentioning

confidence: 77%

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Hunter

2007

Molecular Reproduction Devel

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To avoid inappropriate conclusions being drawn from the extensive use of in vitro preparations of sperm-oviduct epithelial binding, it is recalled that events in the genital tract of mammals are regulated by the gonads, primarily by their changing secretion of steroid hormones. Key observations from in vivo models are used to emphasise the dynamic interactions between viable sperm cells and the caudal (distal) portion of the oviduct isthmus, the site of the functional sperm reservoir. These include (1) preovulatory arrest and epithelial binding of intact sperm cells and thereby suppression of completion of capacitation, (2) peri-ovulatory activation and release from binding of discrete sub-populations of competent spermatozoa, and (3) post-ovulatory liberation of large numbers of spermatozoa. These observations underline the influence of endocrine regulation of sperm binding and release by peri-ovulatory Graafian follicles, a point brought out by the enhanced sperm release prompted by diverse treatments with solutions of progesterone. In the light of this evidence, the suitability of in vitro preparations for clarifying physiological events should be questioned, especially if myosalpingeal catecholamines diffusing out of the autonomic nervous system contribute to sperm activation and/or release. None of this is to infer that sperm cells themselves are without influence on their epithelial binding reaction(s). Nor is it to suggest that in vitro models of sperm-oviduct binding are without relevance to the development of sperm evaluation technologies. However, pre-ovulatory sperm-epithelial binding and a regulated peri-ovulatory release should be seen as vital tactics in the overall strategy of achieving successful monospermic fertilisation. Mol. Reprod. Dev. 75: 167-174, 2008.

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“…Moreover, it has been reported that addition of postovulatory isthmic oviductal fluid significantly increases bicarbonateinduced capacitation of sperm recovered from the oviduct (40). This changing interaction between spermatozoa and oviductal epithelial cells cannot easily be mimicked in in vitro models even in highly specialized research laboratories (106)(107)(108)(109). Even the obtaining of a sufficient supply of stage-specific oviductal fluid would prove too complex and expensive to be used in routine assessments of sperm capacitation processes.…”

Section: Limitations Of In Vitro Systemsmentioning

confidence: 99%

Cytometric solutions in veterinary andrology: Developments, advantages, and limitations

Petrunkina

Harrison²

2011

Cytometry Pt A

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Cytometric methodologies are becoming increasingly important in veterinary andrology as means of assessing sperm function. However, as yet, flow cytometric techniques in veterinary andrology have not kept up in sophistication with those in other areas of biology and medicine. In this brief review, we consider the present state of cytometry in andrological procedures for evaluating the fertility of domestic animal sires. We outline the aspects of sperm physiology, paying particular attention to the changes that take place during the process known as capacitation, which prepares the sperm for interaction with the egg. We then examine briefly but critically the cytometric techniques that are currently in commercial use or are being established in research laboratories for testing sperm characteristics. Current limitations and potential developments in semen assessment are discussed. Recent research knowledge offers possibilities for applying more subtle flow cytometric approaches to distinguish different levels of fertilizing potential in semen samples. For example, linking field fertility data to multiparametric kinetic studies of sperm capacitational changes rather than ''single parameter-single time point'' estimations may reveal that slower rather than rapid changes indicate high fertility. Moreover, the development of multicolor flow cytometric procedures as a means of evaluating multiple functional parameters in individual cells would reduce the uncertainties always inherent in predicting fertility from in vitro sperm evaluation tests. ' 2011 International Society for Advancement of Cytometry Key terms fertility; sperm evaluation; heterogeneity; subpopulations; multicolour cytometry THE main focus of an andrological assessment in veterinary medicine is to ascertain the fertility of a potential sire. Although the most obvious and indeed frequently used general veterinary test is to quantify the number of offspring born to the sire, this is a lengthy and very expensive process. For ''accuracy,'' many inseminations must be carried out, and results must await gestation and birth. Obviously, therefore, development of rapid tests that can be carried out in the laboratory has for long been important. However, as research into the reproductive process has advanced, it has become clear that the life of the fertilizing sperm between its release from the testis and its fusion with the egg is far from simple. Moreover, the population of sperm ejaculated into the female has been revealed as being heterogeneous in functional ability, even if morphologically normal [c.f. reviews (1,2)]. As a result of these findings, tests are being developed to examine functional ability within a heterogeneously responding population (3-5).Given that a normal male ejaculate contains many millions of individual sperm cells, it is natural that these tests are based on cytometry. In this short review, we will outline and comment upon a range of modern and still-developing cytometric methodologies in use or potentially useful for sperm...

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Intracellular calcium and protein tyrosine phosphorylation during the release of bovine sperm adhering to the fallopian tube epithelium in vitro

Cited by 63 publications

References 35 publications

Is there a role for endocannabinoids in sperm–oviduct interaction?

Is there a role for endocannabinoids in sperm–oviduct interaction?

Sperm release from oviduct epithelial binding is controlled hormonally by peri‐ovulatory graafian follicles

Cytometric solutions in veterinary andrology: Developments, advantages, and limitations

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