Intracellular coexpression of CXC- and CC– chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation

Pinto, Sandra N.; Martínez-Romero, Alicia; O’Connor, José-Enrique; Gil-Benso, Rosario; San-Miguel, Teresa; Terrádez, Liria; Monteagudo, Carlos; Callaghan, Robert C.

doi:10.1186/1471-2407-14-118

Cited by 18 publications

(14 citation statements)

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“…Furthermore, coexpression of ligands and chemokine receptors in neoplastic cells and extracellular matrix suggests that autocrine and paracrine stimulation by the tumor cells results in production of angiogenic factors in response to hypoxia during the first stages of tumor growth, as has been reported in other neoplastic and non-neoplastic conditions [13,[20][21][22].…”

Section: Discussionmentioning

confidence: 76%

“…Animal models have been used widely in the study of tumor angiogenesis [11][12][13]. Our aims were to characterization neovascularization of xenotransplanted Os in a nude mouse model at histological, immunohistochemical, ultrastructural, and molecular level, and to consider the use of this model in testing efficacy of new anti-angiogenic drugs.…”

Section: Introductionmentioning

confidence: 99%

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The early stages of tumor angiogenesis in human osteosarcoma: a nude mice xenotransplant model

et al. 2015

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Osteosarcoma (Os) is the most common malignant bone tumor in childhood and not rare in adults. In recent years, much research has focused on the role of angiogenesis in tumor development, growth, invasion, and metastasis. The aims of this study were to characterize neovascularization established between the xenotransplanted Os and the host at histological, immunohistochemical, ultrastructural, and molecular level, and to evaluate if this model could be used in testing new anti-angiogenic drugs. Three xenotransplanted human Os were evaluated. Tumor pieces 3-4 mm in size were implanted subcutaneously on the back of athymic Balb-c nude mice (n = 14). The animals were killed at 24, 48, and 72 h and 7, 14, 21, and 28 days after implantation. Tumor samples were either fixed in 10 % formaldehyde and embedded in paraffin for histological analysis, or fixed with glutaraldehyde (2 %) for electron microscopy or retained non-fixed for molecular analysis (ELISA and qRT-PCR). Morphologically, intense neo-vasculogenesis within tumor parenchyma was present between the first and third week after transplantation. Immunohistochemistry demonstrated overexpression of VEGF and their receptors together with PDFGFRA 24-48 h after tumor implantation. Additionally, neoplastic cells co-expressed chemokines (CXCL9, CXCL10, and GRO) and their receptors. Molecular studies showed two expression profiles, distinguishing an early and a late phase in the angiogenic process. In Os, our model showed two stages of induced angiogenesis, with close association between histological and molecular events. This approximation could be of use for testing the effect of different anti-angiogenic agents.

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Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

The early stages of tumor angiogenesis in human osteosarcoma: a nude mice xenotransplant model

et al. 2015

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“…Several studies have shown that melanoma cell lines co-express high levels of chemokine factor receptors and ligands ( 24 ). Chemokines and tumor cell surface receptors are involved in post-melanoma angiogenesis, tumor cell growth, invasion and metastasis of malignant processes ( 25 ).…”

Section: Discussionmentioning

confidence: 99%

Mechanisms of CXCR7 induction in malignant melanoma development

Liu

Tian

et al. 2017

Oncology Letters

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Malignant melanoma (MM) is a highly malignant skin tumor. The mechanism of MM pathogenesis and its signaling pathways are not well characterized. C-X-C chemokine receptor type 7 (CXCR7) has been reported to regulate cancer cell invasion. The present study sought to investigate the effects of CXCR7 on MM development. First, CXCR7 expression levels were assessed in the skin tumor tissue of patients with MM. Then, CXCR7 small hairpin RNA was used in M14 melanoma cells in a Transwell culture model and in a transplanted mouse model to test the effects of CXCR7. In addition, immunohistochemistry staining, reverse transcription-quantitative polymerase chain reaction and western blotting were used. The results revealed that CXCR7 expression levels were significantly higher in MM tissue compared with squamous cell carcinoma or basal cell carcinoma tissue. Knocking down CXCR7 in M14 cells significantly inhibited cell migration and invasion in the Transwell culture model. Furthermore, CXCR7 knockdown also significantly reduced the transplanted tumor size, weight and vascular number in the mouse model. It was concluded that CXCR7 interacts with C-X-C motif chemokine ligand 12 to activate the chemokine receptor signaling pathway, and to increase melanoma cell migration, invasion and development.

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“…It has become clear that tumour angiogenesis is the result of an imbalance between proangiogenic and antiangiogenic factors, the threshold of change in favour of proangiogenesis is considered to be the angiogenic switch. Several angiogenic factors and chemokines related with angiogenic mechanisms have been studied in different tumour types [6, 8, 9]. We recently communicated these findings in a nude mice osteosarcoma model [10] as well as in two high-grade chondrosarcomas (in press) [11].…”

Section: Introductionmentioning

confidence: 99%

High-risk gastrointestinal stromal tumour (GIST) and synovial sarcoma display similar angiogenic profiles: a nude mice xenograft study

Giner

Machado

Löpez‐Guerrero

et al. 2017

ecancer

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BackgroundGastrointestinal stromal tumour (GIST) is the most common primary mesenchymal tumour of the gastrointestinal tract. Spindle cell monophasic synovial sarcoma (SS) can be morphologically similar. Angiogenesis is a major factor for tumour growth and metastasis. Our aim was to compare the angiogenic expression profiles of high-risk GIST and spindle cell monophasic SS by histological, immunohistochemical and molecular characterisation of the neovascularisation established between xenotransplanted tumours and the host during the initial phases of growth in nude mice.MethodsThe angiogenic profile of two xenotransplanted human soft-tissue tumours were evaluated in 15 passages in nude mice using tissue microarrays (TMA). Tumour pieces were also implanted subcutaneously on the backs of 14 athymic Balb-c nude mice. The animals were sacrificed at 24, 48, and 96 h; and 7, 14, 21, and 28 days after implantation to perform histological, immunohistochemical, and molecular studies (neovascularisation experiments).ResultsMorphological similarities were apparent in the early stages of neoplastic growth of these two soft-tissue tumours throughout the passages in nude mice and in the two neovascularisation experiments. Immunohistochemistry demonstrated overexpression of pro-angiogenic factors between 24 h and 96 h after xenotransplantation in both tumours. Additionally, neoplastic cells coexpressed chemokines (CXCL9, CXCL10, GRO, and CXCL12) and their receptors in both tumours. Molecular studies showed two expression profiles, revealing an early and a late phase in the angiogenic process.ConclusionThis model could provide information on the early stages of the angiogenic process in monophasic spindle cell SS and high-risk GIST and offers an excellent way to study possible tumour response to antiangiogenic drugs.

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Intracellular coexpression of CXC- and CC– chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation

Cited by 18 publications

References 50 publications

The early stages of tumor angiogenesis in human osteosarcoma: a nude mice xenotransplant model

The early stages of tumor angiogenesis in human osteosarcoma: a nude mice xenotransplant model

Mechanisms of CXCR7 induction in malignant melanoma development

High-risk gastrointestinal stromal tumour (GIST) and synovial sarcoma display similar angiogenic profiles: a nude mice xenograft study

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