Intracellular dehydration in the rat made diabetic with streptozotocin: effects of infusion

Abstract

Metabolic and isotopic dilution techniques were used to investigate fluid balance and fluid volumes in rats made diabetic with streptozotocin before and after infusion. Uninfused diabetic rats had significantly (P less than 0.01) lower total body water than controls (57.7 +/- 2.2 vs 65.7 +/- 1.4% (S.E.M.) fat free mass). This was due exclusively to a significantly (P less than 0.001) reduced intracellular fluid volume (38.2 +/- 1.5 vs 45.4 +/- 1.4% fat free mass). Metabolic studies over the preceding 2 weeks showed that the fluid deficit in the diabetic group had resulted from a failure of the rats to increase their fluid intake to the same extent as their combined fluid losses. A 4-h saline infusion halved the fluid deficit in diabetic animals. The retained fluid was used to restore intracellular fluid volume which became comparable in diabetic and control rats (47.2 +/- 2.0 vs 46.4 +/- 1.0% fat free mass). The retention of infusate by diabetic animals to counteract their intracellular dehydration may partly explain the reduced urine output reported elsewhere in infused anaesthetized diabetic rats.

Study of the pharmacokinetic changes of Tramadol in diabetic rats

“…It has been suggested that plasma protein binding of drugs may change because of change in plasma fatty acid levels [9]. Moreover, an intracellular dehydration has been observed in male Sprague-Dawley DMIS rats [24]. Consequently both may affect the distribution of drugs in the body.…”

Insulin Containing Nanocomplexes Formed by Self-Assembly from Biodegradable Amine-Modified Poly(Vinyl Alcohol)-Graft-Poly(l-Lactide): Bioavailability and Nasal Tolerability in Rats

“…In diabetic rats insulin deficiency causes severe changes in metabolism among others, a reduction in liver protein synthesis, decreased activity of the sympathetic nervous system, dehydration, glycosuria, and osmotic diuresis (28,29). Thus, streptozotocin-diabetic rats showed a different response profile to environmental stress factors (30).…”

Renal Function in Chronically Catheterized Conscious Diabetic Rats Using Constant and Servo‐controlled Infusion

“…5,22 Values recorded from our rats clearly represent an overestimate of the animal's natural excretion rate and probably result from a volume expansion produced by the high constant infusion. 23 If they are infused at a rate below that of their natural fluid intake or urine output, fluid is retained rather than excreted in an attempt to restore intracellular volume. 5 When our diabetic rats were similarly infused, their urine output stabilized at 106Ϯ13 L/min, contrasting with rates of approximately twice this value reported in non-infused diabetic animals.…”