Intracellular immunoglobulin in lymphocytes from patients with chronic lymphocytic leukemia: An immunoelectron microscopic study

Secretion of immunoglobulin by neoplastic B lymphocytes from lymph nodes of patients with lymphoma

“…These low levels of secretion have been confirmed by others (Johnstone et al, 1982) and the presence of intracellular IgM in cells from such patients has been detected by using sensitive immunoelectron microscopy (Yasuda et al, 1982). Secretion of free monotypic light chain by the majority of CLL cell populations has also been observed (HannamHarris et al, 1980).…”

Lymphocytic lymphoma/B-chronic lymphocytic leukaemia – An immunohistopathological study of peripheral B lymphocyte neoplasia

“…Increased staining of PC with the monoclonal 33.1 which detects a distinct DC-related Class II molecule (Marti et al, 1983) (Cossman et al, 1983;Forbes et al, 1981;Maeda & Deegan, 1983;Okamura et al, 1982;Totterman et al, 1980Totterman et al, , 1981a. These effects represent, probably, quantitative changes rather than "differentiation" events, in that CLL cells can secrete IgM (Stevenson et al, 1980(Stevenson et al, , 1982, and monoclonal clg has been reported to be present in most cases of CLL (Gordon et al, 1983a;Guglielmi et al, 1982, Han et al, 1982Johnstone et al, 1982;Yasuda et al, 1982;Newell et al, 1983 Martin et al, 1981;Nanba et al, 1977). B-CLL cells are morphologically and phenotypically distinct from germinal centre cells .…”

High Membrane Cholesterol in CLL B-Cells and Differential Expression of Cholesterol Synthesis Genes in IG GENE-Unmutated vs Mutated Cells

“…Previous results from our group led us to hypothesize that CLL B-cells may be enriched in membrane cholesterol, possibly in relation with the expression of CD5; Data from the literature suggested that a high content in cholesterol at the plasma membrane may affect BCR trafficking and signalling (Bléry et al, 2006). Of interest, retention of the BCR in the cytoplasm of CLL cells has long been observed (Yasuda et al, 1982;Newell et al, 1983) suggesting trafficking abnormalities in this pathology. Membrane rigidity in CLL is a prominent cytological feature easily evidenced by the disruption of the cells and numerous Gumprecht's shadows on cytocentrifuged cells stained with May Grünwald Giemsa, and it is noteworthy that an increase in membrane cholesterol increases rigidity (Müller et al, 1970;An et al, 2010).…”