Intracellular Metabolism of 4-Hydroxynonenal in Primary Cultures of Rabbit Synovial Fibroblasts

Ullrich, Oliver; Huser, Hans; Ehrlich, W.; Grune, Tilman

doi:10.1016/s0891-5849(96)00496-0

Cited by 24 publications

(17 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The GSTs identified in this study could possibly be responsive to (1) midgut fermentation products (Ebert et al ., 2003) or (2) oxidative stress in the midgut cells. For example, 4‐hydroxynonenal (4‐HNE) is a highly toxic aldehyde produced by lipid peroxidation in cells in response to oxidative stress (Ullrich et al ., 1997). The major pathway of 4‐HNE metabolism in mammals involves glutathione conjugation by GSTs (Alin et al ., 1985; Videla et al ., 2000).…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic profiles of Drosophila melanogaster third instar larval midgut and responses to oxidative stress

Buczkowski

Mittapalli

et al. 2008

Insect Molecular Biology

View full text Add to dashboard Cite

Oligoarray analysis was used to determine the number and nature of genes expressed in third instar Drosophila melanogaster larval midguts. The majority of transcripts were associated with protein synthesis and metabolism. Serine proteases were the main proteolytic enzymes detected. Some 40% of the cytochrome P450 genes and 74% of the glutathione S transferases (GSTs) in the genome of D. melanogaster were observed to be expressed in the midgut by oligoarray analysis. We also identified potential transcription factor binding motifs (TFBMs) of P450s, GSTs and carboxylesterases. Many of the midgut-expressed GST genes contained candidate TFBMs homologous to TFBMs in mammals that have been associated with responses to oxidative stress. We also investigated the response of GSTs in the midgut to dietary H2O2, which showed a dosage-based differential response.

show abstract

Section: Resultsmentioning

confidence: 99%

Transcriptomic profiles of Drosophila melanogaster third instar larval midgut and responses to oxidative stress

Buczkowski

Mittapalli

et al. 2008

Insect Molecular Biology

View full text Add to dashboard Cite

show abstract

“…This original postulate was later corrected with the identification of a variety of HNE products and the consistent observation of a quantitative prevalence of GSH-adduct(s) and/or HNA as to alcohol DHN. 174 HNE metabolism was also studied in hepatoma cells, 180,181 ascites tumor cells, 182 mucosal cells, 171 synovial fibroblasts, 183 thymocytes, 184 vascular smooth muscle cells (SMC) 185 and also in organs such as heart 186 and kidney. 173,187 Always a rapid HNE degradation and intracellular metabolism was observed.…”

Section: A Investigation Of Hne Metabolism In Mammalian Cells and Ormentioning

confidence: 99%

4‐Hydroxynonenal: A membrane lipid oxidation product of medicinal interest

Poli

Schaur

Siems³

et al. 2007

Medicinal Research Reviews

393

411

View full text Add to dashboard Cite

A comprehensive focus on 4-hydroxynonenal (HNE) as candidate molecule in a variety of pathophysiological conditions occurring in humans is here provided. Despite an active, now well characterized, metabolism in most cells and tissues, HNE can be easily detected and quantified by means of several methods, although with different sensitivity. Measurements of HNE and/or stable metabolites in biological fluids are already applied as lipid peroxidation/oxidative stress markers in a huge number of human disease processes, often sustained by inflammatory reactions. A primary involvement of this aldehydic product of membrane lipid oxidation in inflammation-related events, as well as in regulation of cell proliferation and growth, in necrotic or apoptotic cell death, appears supported by its marked ability to modulate several major pathways of cell signaling and, consequently, gene expression. The actual knowledge of HNE reactivity, metabolism, signaling and modulatory effect in the various human organs should provide a solid background to the investigation of the aldehyde's contribution to the pathogenesis of human major chronic diseases and would likely promote advanced and oriented applications not only in diagnosis and prevention but also in molecular treatment of human diseases.

show abstract

“…HNE METABOLISM, CHEMICAL REACTIVITY, AND HNE-ADDUCT FORMATION IN TISSUES AND CELLS Intracellular steady-state levels of HNE are essentially the result of an equilibrium between its generation and metabolism. Rate and efficiency of HNE metabolism in a defined type of cell can result in complete disposal of this compound, and then in the prevention of major cell injury or response, or in an incomplete removal (Canuto et al, 1994;Ullrich et al, 1994Ullrich et al, , 1997Hartley et al, 1995;Grune et al, 1997a,b;Siems et al, 1997aSiems et al, , 1998Srivastava et al, 1998;Petras et al, 1999). HNE can be transformed by aldehyde dehydrogenase (ALDH) isoforms into the major metabolite 4-hydroxy-nonenoic acid and by alcohol dehydrogenase (ADH) isoforms into 1,4-dihydroxynonene.…”

Section: Introductionmentioning

confidence: 99%

4-Hydroxynonenal As a Biological Signal: Molecular Basis and Pathophysiological Implications

Parola

Bellomo

Robino

et al. 1999

Antioxidants & Redox Signaling

237

171

View full text Add to dashboard Cite

Reactive oxygen intermediates (ROI) and other pro-oxidant agents are known to elicit, in vivo and in vitro, oxidative decomposition of omega-3 and omega-6 polyunsaturated fatty acids of membrane phospholipids (i.e, lipid peroxidation). This leads to the formation of a complex mixture of aldehydic end-products, including malonyldialdehyde (MDA), 4-hydroxy-2,3-nonenal (HNE), and other 4-hydroxy-2,3-alkenals (HAKs) of different chain length. These aldehydic molecules have been considered originally as ultimate mediators of toxic effects elicited by oxidative stress occurring in biological material. Experimental and clinical evidence coming from different laboratories now suggests that HNE and HAKs can also act as bioactive molecules in either physiological and pathological conditions. These aldehydic compounds can affect and modulate, at very low and nontoxic concentrations, several cell functions, including signal transduction, gene expression, cell proliferation, and, more generally, the response of the target cell(s). In this review article, we would like to offer an up-to-date review on this particular aspect of oxidative stress--dependent modulation of cellular functions-as well as to offer comments on the related pathophysiological implications, with special reference to human conditions of disease.

show abstract

Intracellular Metabolism of 4-Hydroxynonenal in Primary Cultures of Rabbit Synovial Fibroblasts

Cited by 24 publications

References 30 publications

Transcriptomic profiles of Drosophila melanogaster third instar larval midgut and responses to oxidative stress

Transcriptomic profiles of Drosophila melanogaster third instar larval midgut and responses to oxidative stress

4‐Hydroxynonenal: A membrane lipid oxidation product of medicinal interest

4-Hydroxynonenal As a Biological Signal: Molecular Basis and Pathophysiological Implications

Contact Info

Product

Resources

About