Intracellular prokaryotes in rumen ciliate protozoa: Detection by confocal laser scanning microscopy after in situ hybridization with fluorescent 16S rRNA probes

Lloyd, David; Williams, Alan G.; Amann, R.; Hayes, Alan; Durrant, Lisa; Ralphs, J. R.

doi:10.1016/s0932-4739(96)80011-3

Cited by 51 publications

(47 citation statements)

References 34 publications

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“…Broiler drinking water systems provide low-oxygen or anoxic habitats for microbes, explaining the detection of anoxic unculturable alveolate clones. Although reports of intracellular prokaryotes in rumen ciliate protozoa (anoxic environment) exist, the potential of anoxic uncultured alveolate clones to act as Campylobacter hosts is unknown but nevertheless may exist (9,43,44). Protozoa were detected in the water systems of all farms, thus indicating the potential for Campylobacter and protozoa to interact.…”

Section: Resultsmentioning

confidence: 96%

“…This may have been because the protozoa were feeding at a high rate after being starved. Initially fewer bacteria were recovered from cocultures containing T. pyriformis than A. castellanii, probably because T. pyriformis moves at a much higher rate and would be expected to be a more active bacterial predator (9,37,43,44). After 5 days more notable differences existed between the Campylobacter viable counts (Fig.…”

Section: Delayed Decline Of Campylobacter Viability In Coculturesmentioning

confidence: 98%

See 1 more Smart Citation

Survival of Campylobacter jejuni in Waterborne Protozoa

Snelling¹,

McKenna²,

Lecky³

et al. 2005

Appl Environ Microbiol

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The failure to reduce the Campylobacter contamination of intensively reared poultry may be partially due to Campylobacter resisting disinfection in water after their internalization by waterborne protozoa. Campylobacter jejuni and a variety of waterborne protozoa, including ciliates, flagellates, and alveolates, were detected in the drinking water of intensively reared poultry by a combination of culture and molecular techniques. An in vitro assay showed that C. jejuni remained viable when internalized by Tetrahymena pyriformis and Acanthamoeba castellanii for significantly longer (up to 36 h) than when they were in purely a planktonic state. The internalized Campylobacter were also significantly more resistant to disinfection than planktonic organisms. Collectively, our results strongly suggest that protozoa in broiler drinking water systems can delay the decline of Campylobacter viability and increase Campylobacter disinfection resistance, thus increasing the potential of Campylobacter to colonize broilers.

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Section: Resultsmentioning

confidence: 96%

Section: Delayed Decline Of Campylobacter Viability In Coculturesmentioning

confidence: 98%

Survival of Campylobacter jejuni in Waterborne Protozoa

Snelling¹,

McKenna²,

Lecky³

et al. 2005

Appl Environ Microbiol

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show abstract

“…Similarly, as in the case of bacteria, the degradation of crude fiber being the effect of protozoan activity results in an increased concentration of hydrogen in the ruminal environment, which is used in the so-called horizontal hydrogen transfer by methanogens to the reduction of carbon (IV) oxide. Vogels et al (1980) and Lloyd et al (1996) showed that cells of ciliates may be colonized by Archaea. This indicates that the population of methanogens is connected with the numbers of protozoans.…”

Section: Rumen Protozoamentioning

confidence: 99%

Dietary Possibilities to Mitigate Rumen Methane and Ammonia Production

Szumacher-Strabel¹,

Cieślak²

2012

Greenhouse Gases - Capturing, Utilization and Reduction

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“…The Archaea, which live in the digestion system of ruminants, are very difficult to cultivate in vitro (Garcia et al, 2000). Moreover, in the research, where FISH was used, the association of the methanogens with rumen ciliates was showed (Lloyd et al, 1996). Because FISH does not require the pure culture of microorganisms, it can be used in the determination of microbial mixed population in the rumen.…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research

Pers‐Kamczyc¹,

Zmora²,

Cieślak³

et al. 2011

J. Anim. Feed Sci.

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Despite many years of research elucidating processes occurring in the rumen, still only around 10% of the rumen microbe is known. Molecular biology techniques seem to be a good complement of an information obtained by using the classical microbiology and therefore can enhance our knowledge about processes occurring in the rumen. The aim of this paper is to describe and to summarize a widely used techniques of the molecular biology during analysis of microbes in rumen. The main attention have been focused on the three of them which can be employed for quantitative and qualitative analysis of rumen microorganisms: PCR-DGGE, Real-time PCR and Fluorescence in situ hybridization.

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Intracellular prokaryotes in rumen ciliate protozoa: Detection by confocal laser scanning microscopy after in situ hybridization with fluorescent 16S rRNA probes

Cited by 51 publications

References 34 publications

Survival of Campylobacter jejuni in Waterborne Protozoa

Survival of Campylobacter jejuni in Waterborne Protozoa

Dietary Possibilities to Mitigate Rumen Methane and Ammonia Production

Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research

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