Intracellular uptake, absorption spectrum and stability of the bacteriochlorin photosensitizer 5,10,15, 20-tetrakis (m-hydroxyphenyl) bacteriochlorin (mTHPBC). Comparison with 5,10,15,20-tetrakis (m-hydroxyphenyl) chlorin (mTHPC)

Physical and chemical studies related to the development of m-THPC (FOSCAN®) for the photodynamic therapy (PDT) of tumours

“…Aronoff [29] carried out perchloric acid titrations spectrophotometrically with 5,10,15,20-tetraphenylporphyrin (TPP) and 5,10,15, 20-tetraphenylchlorin in nitrobenzene solutions. As in the present case, he found that the porphyrin gave a single feature (which he nonetheless analysed in terms of pK 3 4.4 and pK 4 3.9) while the chlorin gave a clear intermediate stage, i.e. the monocation.…”

“…Although the bacteriochlorin was in fact more active than the chlorin in vivo, further study and development of it at that time was discounted because it was more difficult to prepare and because it was more readily oxidised, which had implications for shelf life. (However, it has more recently attracted renewed attention [3].) A comparative study of what were considered to be the four best photosensitisers discovered by our group at that time and of Photofrin II was carried out [4].…”

Indocyanine Green Nanoparticles Useful for Photomedicine

“…1 The ideal PDT photosensitizer must be taken up by cells, and it must also absorb light at wavelengths that fall within the red region of the spectrum (650 and 800 nm), known as a therapeutic window, where maximal penetration of light into tissues is observed. 2 The recent interest in using indocyanine green (ICG) as a photosensitizer in PDT comes from the fact that this dye has a strong absorption band around 780 nm 3 , which falls in the "photodynamic window." 4,5 The ICG molecule exhibits a molecular structure with amphiphilic properties that has both hydrophilic and lipophilic properties 6,7 (Fig.…”

Tissue Uptake Study and Photodynamic Therapy of Melanoma‐Bearing Mice with a Nontoxic, Effective Chlorin

“…In addition to the good spectroscopic properties of foscan, notably an e 650 value of 29 600 m À1 cm À1 , it has a reasonable singlet oxygen quantum yield in air-saturated methanol (F D = 0.43). [13] The analogous bacteriochlorin (THPB) has still better spectroscopic and photochemical properties (e 735 = 91 000 m À1 cm À1 ; F D = 0.43 in air-saturated ethanol), [13] but in vitro phototoxicity towards the mouse tumor cell line colon 26 [14] or the depth of tumor photonecrosis 24 h after intraperitoneal (i.p.) injection [15] did not reflect the increase in singlet oxygen generation for an equivalent light dose.…”