2002
DOI: 10.1016/s0891-0618(01)00152-1
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Intracerebroventricular infusion of neuropeptide Y up-regulates synthesis and accumulation of luteinizing hormone but not follicle stimulating hormone in the pituitary cells of prepubertal female lambs

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Cited by 17 publications
(7 citation statements)
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“…Paraffinembedded pituitary sections were mounted on slides and coated with 3-aminopropyltriethoxysilane (2% in acetone; Sigma, St. Louis, MO, USA). The nonradioactive in situ hybridization procedure using DIG-labelled cDNA probes was performed according to the Breitschopf et al, (1992) protocol, with minor modifications by Wańkowska et al (2002). Briefly, slides were deparaffinised, rehydrated, washed in PBS, fixed with 4% paraformaldehyde in PBS and treated with 0.25% acetic anhydride in 100 mM TEA buffer (triethanolamine-Cl; Sigma).…”
Section: Hybridohistochemistrymentioning
confidence: 99%
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“…Paraffinembedded pituitary sections were mounted on slides and coated with 3-aminopropyltriethoxysilane (2% in acetone; Sigma, St. Louis, MO, USA). The nonradioactive in situ hybridization procedure using DIG-labelled cDNA probes was performed according to the Breitschopf et al, (1992) protocol, with minor modifications by Wańkowska et al (2002). Briefly, slides were deparaffinised, rehydrated, washed in PBS, fixed with 4% paraformaldehyde in PBS and treated with 0.25% acetic anhydride in 100 mM TEA buffer (triethanolamine-Cl; Sigma).…”
Section: Hybridohistochemistrymentioning
confidence: 99%
“…Reproductive functions are highly energy consuming, therefore, sophisticated mechanisms have been selected during evolution to allow for the reduction of HPG axis activity under energy insufficiency. Several neuroendocrine factors like neuropeptide Y, leptin, insulin and kisspeptin interact in the physiological control of energy balance and reproductive processes (Wańkowska et al, 2002;Fernandez-Fernandez et al, 2006;TenaSempere, 2007;Wójcik-Gładysz et al, 2009). Ghrelin is a 28-amino acid hormone identified as an endogenous ligand for the growth hormone secretagogue receptor (GHS-R) type 1a (Kojima et al, ABSTRACT.…”
Section: Introductionmentioning
confidence: 99%
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“…All sections from every stage were run in a single assay. The non-radioactive in situ hybridisation procedure using DIG-labelled cDNA probes was performed according to Breitschopf et al (1992) protocol, with minor modifications by Wańkowska et al (2002). Briefly, slides were deparaffined, rehydrated, washed in PBS, fixed with 4% paraformaldehyde in PBS and treated with 0.25% acetic anhydride in 100 mM TEA buffer (triethanolamine-Cl; Sigma, St. Louis, USA).…”
Section: Hybridohistochemistrymentioning
confidence: 99%
“…The role of NPY in the regulation of somatostatin in sheep has only been investigated under conditions of food deprivation, indicating that this hypothalamic peptide could mediate a nutritionally dependent decrease in the release of somatostatin (Polkowska and Gladysz, 2001). NPY is also involved in the stimulation of prepubertal events in lambs by enhancing LH secretory processes in this period (Wań kowska et al, 2002). It can be supposed that this action is mediated through the ARC somatostatin population of neurons involved in prepubertal events (Polkowska et al, 2008).…”
Section: Introductionmentioning
confidence: 99%