The effect of intracerebroventricular infusions of ghrelin
on the secretory activity of the GnRH/LH system
in peripubertal ewes

Wójcik-Gładysz, Anna; Wańkowska, Marta; Gajewska, Alina; Misztal, Tomasz; Szlis, Michał; Polkowska, Jolanta

doi:10.22358/jafs/65665/2014

Cited by 6 publications

(5 citation statements)

References 45 publications

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“…Generally, in Polish Merino sheep oestrous behaviour occurred between 33 and 34 weeks of age. Our previous observations confirmed that under normal environmental and nutritional conditions, the onset of puberty in Polish Merino lambs born in early February occurs at the age of 33 weeks (Wójcik‐Gładysz et al., ). The animals were randomly divided into two experimental groups: a control group, receiving an intracerebroventricular (icv) infusion of Ringer‐Locke solution (artificial cerebrospinal fluid, 120 μl/hr, n = 14), and an obestatin group, receiving an icv infusion of obestatin (Goat/Ovine Obestatin, Phoenix Pharmaceuticals Inc, Belmont, CA) diluted in Ringer‐Locke solution ( n = 14) for a total dose of 100 μg/day, at infusion rate of 120 μl/hr.…”

Section: Methodssupporting

confidence: 87%

“…Moreover, NPY neurons in the ARC nucleus are primary targets for many orexigenic and anorexigenic peripheral hormones (Gładysz, Krejci, Simůnek, & Polkowska, ; Tillet et al., ). Our previous studies confirmed that peripheral peptides, such as leptin and ghrelin, are involved in the modulation of hypothalamic appetite‐regulating network activity in sheep, and this modulation occurs via NPY neurons in the ARC nucleus (Polkowska, Gajewska, Wańkowska, Misztal, & Wójcik‐Gładysz, ; Tillet et al., ; Wójcik‐Gładysz, Wańkowska, Misztal, Romanowicz, & Polkowska, ; Wójcik‐Gładysz et al., ). Considering above information, at least one hypothalamic pathway consists of NPY neurons, and the NPY neuronal system integrates the final pathway regulating food intake (Schneeberger, Gomis, & Claret, ).…”

Section: Introductionsupporting

confidence: 73%

“…Immediately after the last infusion, the animals were weighed, anaesthetized intravenously using dexmedetomidine (0.05 ml/kg, Dexdomitor ® , Orion Pharma, Turku, Finland) and pentobarbital (80 ng/kg, Morbital ® , Vetoquinol Biowet, Poland) and decapitated. Two previously described methods (Wójcik‐Gładysz et al., ) for preserving collected tissues (freezing in liquid nitrogen and in situ fixation) were used in each experimental group. For molecular biology analysis, hypothalamic structures (mediobasal hypothalamus area [MBH] containing periventricular nucleus [PEV] and ARC nucleus as well as median eminence [ME]) from seven sheep from both groups ( n = 7 + 7) were dissected.…”

Section: Methodsmentioning

confidence: 99%

“…Two previously described methods(Wójcik-Gładysz et al, 2014) forpreserving collected tissues (freezing in liquid nitrogen and in situ fixation) were used in each experimental group. For molecular biology analysis, hypothalamic structures (mediobasal hypothalamus area [MBH] containing periventricular nucleus [PEV] and ARC nucleus as well as median eminence [ME]) from seven sheep from both groups (n = 7 + 7) were dissected.…”

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confidence: 99%

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Does obestatin modulate the hypothalamic appetite‐regulating network in peripubertal sheep?

Szlis

Polkowska

Skrzeczyńska

et al. 2018

Animal Physiology Nutrition

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The participation of peripheral peptides in the processes regulating the food intake (energy homeostasis) at the central nervous system level remains unclear. This study focuses on the role of obestatin in neuronal activity within the hypothalamic appetite-regulating network in ruminants. The animals (n = 28) were randomly divided into two groups. The sheep in the control group received intracerebroventricular infusions of the Ringer-Locke solution, and the sheep in obestatin group were infused with obestatin (diluted in the Ringer-Locke solution) at 25 μg per 120 μl/hr. The series of four 1-hr infusions on 3 consecutive days were performed, and immediately after the experiment, the sheep were decapitated. Selected brain regions were fixed in situ for further immunohistochemical analysis, while the remaining ones were frozen for real-time RT-qPCR analysis. Obestatin infusion elicited changes in the neuropeptide Y (NPY) neuronal network in the hypothalamus. The results obtained show that exogenous obestatin evoked an increase in npy and agrpmRNA expression in the mediobasal hypothalamus (MBH), while the immunoreactivity for NPY was decreased in the arcuate and periventricular nuclei. The increase in cart and pomcmRNA expression in the MBH was also observed. Moreover, increased levels of gpr39 receptor and npy receptor 1 mRNA expression were evident in obestatin-infused sheep. Based on these results, it can be concluded that obestatin plays a role in the modulation of appetite-regulating network at the central level in sheep. The results obtained suggest that the underlying mechanism may involve the modification of the activity of NPY/AgRP and CART/α-MSH neurons in the arcuate nucleus.

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Section: Methodssupporting

confidence: 87%

Section: Introductionsupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Does obestatin modulate the hypothalamic appetite‐regulating network in peripubertal sheep?

Szlis

Polkowska

Skrzeczyńska

et al. 2018

Animal Physiology Nutrition

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“…Neuropeptide Y (NPY), agouti-related peptide (AgRP) and proopiomelanocortin (POMC) mRNA expression increased after BDNF intracerebroventricular (ICV) infusion (Przybył et al, 2020). On the other hand, our previous research demonstrated that several factors involved in appetite regulation processes, such as NPY, ghrelin and obestatin, can change the somatotrophic axis hormones secretory activity in sheep (Wójcik-Gładysz et al, 2014Szlis et al, 2018). It is well-known that the activity of the somatotrophic axis in the CNS may also be modulated by several factors, such as drugs, antibiotics and neurotrophins (Timmusk et al, 1993;Patapoutian and Reichardt, 2001;Kennedy, 2012).…”

Section: Introductionmentioning

confidence: 99%