Intracistronic heterogeneity of the 16S rRNA locus in Taylorella equigenitalis isolated from cases of contagious equine metritis

Moore, John E.; Millar, B.C.; Matsuda, Motoo; Haga, Setsuko; Murayama, Ohoshi; Kagawa, Shizuko; Matsui, Toshihiro

doi:10.1016/s0737-0806(02)70206-2

Cited by 2 publications

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“…In the present study, we also determined the sequences of the 16S rDNA from three strains of T. equigenitalis in which sequence di¡erences among the strains occurred at only a few nucleotide positions and described the intracistronic heterogeneity of the 16S rRNA locus in T. equigenitalis isolated from cases of CEM elsewhere (Moore et al, 2002). Bleumink-Pluym and colleagues developed and evaluted a PCR system for the detection of T. equigenitalis using a genus-speci¢c primer^probe set designed by the alignment of the 16S rDNA sequences of di¡erent bacterial species, including T. equigenitalis (Bleumink-Pluym et al, 1994).…”

Section: Discussionmentioning

confidence: 94%

Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)

et al. 2006

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The primer set for 16S rDNA amplified an amplicon of about 1500 bp in length for three strains of Taylorella equigenitalis (NCTC11184(T), Kentucky188 and EQ59). Sequence differences of the 16S rDNA among the six sequences, including three reference sequences, occurred at only a few nucleotide positions and thus, an extremely high sequence similarity of the 16S rDNA was first demonstrated among the six sequences. In addition, the primer set for 16S-23S rDNA internal spacer region (ISR) amplified two amplicons about 1300 bp and 1200 bp in length for the three strains. The ISRs were estimated to be about 920 bp in length for large ISR-A and about 830 bp for small ISR-B. Sequence alignment of the ISR-A and ISR-B demonstrated about 10 base differences between NCTC11184(T) and EQ59 and between Kentucky188 and EQ59. However, only minor sequence differences were demonstrated between the ISR-A and ISR-B from NCTC11184(T) and Kentucky188, respectively. A typical order of the intercistronic tRNAs with the 29 nucleotide spacer of 5'-16S rDNA-tRNA(Ile)-tRNA(Ala)-23S rDNA-3' was demonstrated in the all ISRs. The ISRs may be useful for the discrimination amongst isolates of T. equigenitalis if sequencing is employed.

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Section: Discussionmentioning

confidence: 94%