Intracytoplasmic sperm injection: An assisted reproductive technique and its outcome to overcome infertility

Sarkar, Nandini

doi:10.1080/01443610701327339

Cited by 16 publications

(7 citation statements)

References 41 publications

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“…With the application of in vitro fertilization (IVF), abnormalities in the process of fertilization can be evaluated. In most of the patients with defects in sperm‐egg fusion, fertilization failure can be rescued by intracytoplasmic sperm injection (ICSI) . However, there are still some affected individuals suffering from recurrent fertilization failure even after ICSI attempts and even though morphologically normal oocytes and sperm can be retrieved …”

Section: Introductionmentioning

confidence: 99%

“…In most of the patients with defects in sperm-egg fusion, fertilization failure can be rescued by intracytoplasmic sperm injection (ICSI). [2][3][4] However, there are still some affected individuals suffering from recurrent fertilization failure even after ICSI attempts and even though morphologically normal oocytes and sperm can be retrieved. 5 A normal mature oocyte is critical for successful fertilization.…”

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confidence: 99%

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Novel mutations in WEE2: Expanding the spectrum of mutations responsible for human fertilization failure

Zhang

Zhao

et al. 2019

Clinical Genetics

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Successful fertilization is fundamental for sexual reproduction. After undergoing a series of molecular and morphological changes, the haploid sperm fuses with the haploid oocyte to create a diploid zygote. Defects in this process might lead to human fertilization failure. We have previously found homozygous mutations in WEE2 that are responsible for human fertilization failure, but the genetic basis of human fertilization failure requires further investigation. In the present study, we screened for WEE2 mutations in a new cohort of patients with fertilization failure. Through Sanger sequencing of WEE2 exons, we identified seven novel mutations and two reported mutations in WEE2 from six affected individuals. Morphologically normal PB1 oocytes can be retrieved from all patients. However, most of the oocytes cannot be fertilized successfully. These findings confirmed our previous research and expanded the mutational spectrum of WEE2, making it a potential genetic diagnostic marker for those suffering from human fertilization failure.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Novel mutations in WEE2: Expanding the spectrum of mutations responsible for human fertilization failure

Zhang

Zhao

et al. 2019

Clinical Genetics

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“…With the development of micromanipulation, ICSI has rapidly become an important treatment choice for male infertility (Sarkar, ). Despite the high success rate of ICSI, the potential risks of this treatment are still being evaluated.…”

Section: Introductionmentioning

confidence: 99%

Comparison of conventionalin vitrofertilisation and intracytoplasmic sperm injection outcomes in patients with moderate oligoasthenozoospermia

Shuai

Huang

et al. 2014

Andrologia

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The method of choice for assisted reproductive technology treatment in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI) is usually based on the evaluation of male infertility factors. Decisions for couples with moderate oligoasthenozoospermia (OA) are often empirical because uniform treatment criteria are lacking. This study aimed to evaluate the effect of patients with moderate OA treated with conventional IVF and ICSI. A total of 199 couples with moderate OA undergoing their first IVF/ICSI cycle were included in the study. The patients were divided into two groups according to the type of insemination: conventional IVF group (n = 97) and ICSI group (n = 102). All patients were randomised to be inseminated either by conventional IVF or ICSI. The fertilisation rate, embryo quality, implantation rate and clinical pregnancy rate were examined. No differences in the fertilisation, implantation and pregnancy rates were observed between conventional IVF and ICSI groups (P > 0.05). However, the number of good-quality embryos was significantly higher in the ICSI group than in the IVF group (P < 0.05). Couples with moderate OA did not influence on the overall clinical outcomes between IVF and ICSI treatments, and a negative influence by ICSI on blastocyst development was not confirmed.

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“…Compared with conventional in-vitro fertilization (IVF), ICSI greatly reduces the requirements for sperm quantity, motility, and fertilization ability. With the development of micromanipulation, ICSI has become an important part of assisted reproductive techniques (ARTs) for the treatment of cases with male factor infertility (Sarkar, 2007). With ICSI, an egg can be fertilized not only with fresh sperm with mild or moderate abnormalities but also with sperm following a frozen-thawed procedure, sperm of a severely or extremely severely abnormal concentration, motility or morphology, or even sperm that have been surgically retrieved from the epididymis or testis (van Steirteghem et al, 1993;Devroey et al, 1994;Redgment et al, 1994;Tournaye et al, 1994;Chen et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation

Gao

et al. 2012

J. Zhejiang Univ. Sci. B

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To evaluate the effects of sperm with different parameters and sources on the outcomes of intracytoplasmic sperm injection (ICSI), 1972 ICSI cycles were analyzed retrospectively. Groups 1 to 5 were composed of cycles using ejaculated sperm and were grouped according to sperm quantity, quality, and morphology into normal (288 cycles), or mild (329 cycles), moderate (522 cycles), severe (332 cycles), and extremely severe (171 cycles) oligozoospermia and/or asthenozoospermia and/or teratozoospermia (OAT) groups. Group 6 was composed of 250 cycles using testicular or epididymal sperm, and Group 7 consisted of 80 cycles using frozen-thawed sperm. We found that fertilization rates were gradually reduced from Groups 1 to 6, and reached statistical difference in Groups 5 and 6 (P<0.05). The high-quality embryo rate was higher in Group 1 than in Groups 2, 3, 5, 6, and 7 (P<0.05). No statistical differences were observed in the rates of embryo cleavage, clinical pregnancy, miscarriage, live-birth, premature birth, low birth weight, weeks of premature birth, average birth weight, or sex ratio for all seven groups (P>0.05). A total of nine cases of malformation were observed, with a malformation rate of 1.25% (9/719). In conclusion, different sperm sources and parameters can affect ICSI outcomes before embryo implantation. A full assessment of offspring malformation will require further study using a larger sample size.

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Intracytoplasmic sperm injection: An assisted reproductive technique and its outcome to overcome infertility

Cited by 16 publications

References 41 publications

Novel mutations in WEE2: Expanding the spectrum of mutations responsible for human fertilization failure

Novel mutations in WEE2: Expanding the spectrum of mutations responsible for human fertilization failure

Comparison of conventionalin vitrofertilisation and intracytoplasmic sperm injection outcomes in patients with moderate oligoasthenozoospermia

Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation

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