Intradermal injection of Hsp60 induces cytokine responses in canine atopic and healthy skin

Abstract

The purpose of this study was to investigate the immunoregulatory potential of Hsp60 in the skin of dogs with atopic dermatitis. Three dogs with chronic atopic dermatitis and four healthy dogs were injected intradermally with Hsp60 and phosphate-buffered saline. Biopsies were taken before testing from non-injected control skin, lesional and non-lesional atopic skin, and 48 and 72 h after injection. Analysis of cytokine messenger RNA was performed using quantitative real-time polymerase chain reaction. Forty-eight hours after Hsp60 injection, a rise in interleukin (IL)-10 was found (P=0.034) with the highest expression levels in non-lesional atopic and control skin. A rise of transforming growth factor beta (P=0.015) and IL12p40 (P=0.017) was noticed 72 h after Hsp60 injection in control skin. No significant differences were observed for the expression of IL-4, IL-12p35, and interferon gamma. The results indicate that Hsp60 is able to induce cytokines of a regulatory and Th1 phenotype in the skin. Furthermore, this study seems to provide a first indication of deficient Hsp60 response in atopic dermatitis affected skin.

Recognition of self-heat shock protein 60 by T cells from patients with atopic dermatitis

“…This is the first study to demonstrate that hsp60 is present at the site of inflammation and that it induces specific proinflammatory T cell responses in children with AD. This is in line with a previous experimental study showing that hsp60 can induce cytokines of a regulatory and T H 1 phenotype in the skin of dogs with AD (Jassies-van der Lee et al 2008). This finding is an important condition in investigating novel targets for immunomodulation.…”

Review: Innate immunity, lipid metabolism and nutrition in canine atopic dermatitis

“…However, this was greatest in healthy and nonlesional canine AD skin, and least in lesional AD skin . Heat shock protein 60 also increased expression of the regulatory cytokine transforming growth factor β and the T helper 1 cytokine subunit IL‐12p35 in healthy skin, but not in either lesional or nonlesional atopic skin . These results imply that HSP60 induces a regulatory or mixed regulatory–T helper 1 response in healthy canine skin but that atopic skin (particularly lesional skin) could be refractory to this regulation.…”

“…Intradermal injection of HSP60 induced mRNA expression of the (typically) regulatory cytokine IL‐10 in the skin of both healthy dogs and dogs with AD. However, this was greatest in healthy and nonlesional canine AD skin, and least in lesional AD skin . Heat shock protein 60 also increased expression of the regulatory cytokine transforming growth factor β and the T helper 1 cytokine subunit IL‐12p35 in healthy skin, but not in either lesional or nonlesional atopic skin .…”

Review: The role of antibodies, autoantigens and food allergens in canine atopic dermatitis

“…with or without detectable allergen‐specific IgE) . In some dogs, the diagnosis of AD was made based upon clinical signs alone; in others, the method of diagnosis (specifically, whether the presence of allergen‐specific IgE was determined directly in serum or indirectly via IDT) was not mentioned or was unclear . On the face of it, such distinctions seem to matter little, because AD is generally considered to be a clinical diagnosis in dogs (largely because of the variabilities in IgE reactivity discussed above).…”