Intrafollicular lipopolysaccharide injection delays ovulation in cows

Gindri, Patricia Carvalho; Castro, Natália de Ávila; Mion, B.; Gasperin, Bernardo Garziera; Pegoraro, L. M. C.; Rincón, Joao Alveiro Alvarado; Vieira, Arnaldo Diniz; Pradieé, J.; Pfeifer, Luiz Francisco Machado; Corrêa, Márcio Nunes; Schneider, Augusto

doi:10.1016/j.anireprosci.2019.106226

Cited by 12 publications

(13 citation statements)

References 35 publications

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“…We used the LPS-induced model to verify anovulation caused by inflammation. Delayed ovulation due to intrafollicular LPS has been reported in cows [ 13 , 23 ]. However, no study has reported anovulation in response to an i.m.…”

Section: Discussionmentioning

confidence: 99%

Effect of Foot-and-Mouth Disease Vaccination on Acute Phase Immune Response and Anovulation in Hanwoo (Bos taurus coreanae)

Kim¹,

Moon²,

Ha³

et al. 2021

Vaccines

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Vaccination against foot-and-mouth disease is the most common method for preventing the spread of the disease; the negative effects include miscarriage, early embryo death, lower milk production, and decreased growth of fattening cattle. Therefore, in this study, we analyze the side effects of vaccination by determining the acute immune response and ovulation rate after vaccinating cows for foot-and-mouth disease. The test axis was synchronized with ovulation using 100 Hanwoo (Bos taurus coreanae) cows from the Gyeongsangbuk-do Livestock Research Institute; only individuals with estrus confirmed by ovarian ultrasound were used for the test. All test axes were artificially inseminated 21 days after the previous estrus date. The control group was administered 0.9% normal saline, the negative control was injected intramuscularly with lipopolysaccharide (LPS; 0.5 µg/kg), and the test group was administered a foot-and-mouth disease virus vaccine (FMDV vaccine; bioaftogen, O and A serotypes, inactivated vaccine) 2, 9, and 16 days before artificial insemination. White blood cells and neutrophils increased significantly 1 day after vaccination, and body temperature in the rumen increased for 16 h after vaccination. Ovulation was detected 1 day after artificial fertilization by ovarian ultrasound. The ovulation rates were as follows: control 89%, LPS 60%, FMDV vaccine (−2 d) 50%, FMDV vaccine (−9 d) 75%, and FMDV vaccine (−16 d) 75%. In particular, the FMDV vaccine (−2 d) test group confirmed that ovulation was delayed for 4 days after artificial insemination. In addition, it was confirmed that it took 9 days after inoculation for the plasma contents of haptoglobin and serum amyloid A to recover to the normal range as the main acute immune response factors. The conception rate of the FMDV vaccine (−2 d) group was 20%, which was significantly lower than that of the other test groups.

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Section: Discussionmentioning

confidence: 99%

Effect of Foot-and-Mouth Disease Vaccination on Acute Phase Immune Response and Anovulation in Hanwoo (Bos taurus coreanae)

Kim¹,

Moon²,

Ha³

et al. 2021

Vaccines

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“…Granulosa cell apoptosis appears to be an essential part of ovarian development, and it is a reflection of the mitogenic growth of the follicle [9]. Previous studies have elaborated that lipopolysaccharides (LPS) could induce an ovarian inflammatory response and affect ovarian development directly [10,11].…”

Section: Introductionmentioning

confidence: 99%

UFL1 Alleviates LPS-Induced Apoptosis by Regulating the NF-κB Signaling Pathway in Bovine Ovarian Granulosa Cells

Wang

et al. 2020

Biomolecules

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Ubiquitin-like modifier 1 ligating enzyme 1 (UFL1) is an E3 ligase of ubiquitin fold modifier 1 (UFM1), which can act together with its target protein to inhibit the apoptosis of cells. Lipopolysaccharides (LPS) can affect the ovarian health of female animals by affecting the apoptosis of ovarian granulosa cells. The physiological function of UFL1 on the apoptosis of bovine (ovarian) granulosa cells (bGCs) remains unclear; therefore, we focused on the modulating effect of UFL1 on the regulation of LPS-induced apoptosis in ovarian granulosa cells. Our study found that UFL1 was expressed in both the nucleus and cytoplasm of bGCs. The results here demonstrated that LPS caused a significant increase in the apoptosis level of bGCs in cows, and also dramatically increased the expression of UFL1. Furthermore, we found that UFL1 depletion caused a significant increase in apoptosis (increased the expression of BAX/BCL-2 and the activity of caspase-3). Conversely, the overexpression of UFL1 relieved the LPS-induced apoptosis. In order to assess whether the inhibition of bGCs apoptosis involved in the nuclear factor-κB (NF-κB) signaling pathway resulted from UFL1, we detected the expression of NF-κB p-p65. LPS treatment resulted in a significant upregulation in the protein concentration of NF-κB p-p65, and knockdown of UFL1 further increased the phosphorylation of NF-κB p65, while UFL1 overexpression significantly inhibited the expression of NF-κB p-p65. Collectively, UFL1 could suppress LPS-induced apoptosis in cow ovarian granulosa cells, likely via the NF-κB pathway. These results identify a novel role of UFL1 in the modulation of bGC apoptosis, which may be a potential signaling target to improve the reproductive health of dairy cows.

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“…However, the probability that a cow becomes pregnant following its first insemination postpartum is four times lower in cows that suffered an infection than it is in healthy individuals (Gobikrushanth et al, 2016). This is possibly due to the presence of high intra-follicular concentrations of endotoxins (Cheong et al, 2017), since the intra-follicular injection of bacterial products (lipopolysaccharides) reduces the number of ovulations (Gindri et al, 2019).…”

Section: Uterine Health Of Postpartum Cows and Its Effect On Reproductive Functionmentioning

confidence: 99%

Reproductive activity of dairy cattle in the postpartum anestrus period

González-Maldonado¹,

Hernández²,

Ortiz

et al. 2021

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Objective: To review the metabolic and hormonal processes that regulate reproductive activity in dairy cattleduring the postpartum anestrus period.Design/methodology/approach: Analysis of scientific documents and systematization of information relatedto the reproductive physiology and management of dairy cattle in postpartum anestrus.Results: Postpartum anestrus is an event that occurs naturally and is necessary for restoration of the homeostasisof the cow’s body following calving. However, failure to reestablish this homeostasis actually increases itsduration and negatively affects the subsequent reproductive performance of the individual cow.Limitations on study/implications: Reproductive management decisions must be based on scientificknowledge.Findings/conclusions: Management of dairy cattle in the postpartum anestrus period should focus onreestablishment of the state of homeostasis and the subsequent reproductive activity, with nutritional andhormonal strategies implemented to induce cyclicity, ovulation and formation of the first corpus luteum in thatperiod, through application of exogenous hormones after day ten postpartum.

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Intrafollicular lipopolysaccharide injection delays ovulation in cows

Cited by 12 publications

References 35 publications

Effect of Foot-and-Mouth Disease Vaccination on Acute Phase Immune Response and Anovulation in Hanwoo (Bos taurus coreanae)

Effect of Foot-and-Mouth Disease Vaccination on Acute Phase Immune Response and Anovulation in Hanwoo (Bos taurus coreanae)

UFL1 Alleviates LPS-Induced Apoptosis by Regulating the NF-κB Signaling Pathway in Bovine Ovarian Granulosa Cells

Reproductive activity of dairy cattle in the postpartum anestrus period

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