Intrahost genetic variability of human herpes virus‐8

Leão, Jair Carneiro; Faria, Andreza Barkokebas Santos De; Fonseca, Déborah Daniella Diniz; Gueiros, Luiz Alcino Monteiro; Porter, Stephen

doi:10.1002/jmv.23491

Cited by 10 publications

(10 citation statements)

References 65 publications

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“…1B, gray area). In fact, most studies consistently demonstrate that co-infection by different HHV-8 genotypes is extremely rare, [26,46,50–52] thus supporting that the currently detected recombinant forms do not originate from new events, but they represent previous circulating recombinant forms. [2,49] …”

Section: Discussionmentioning

confidence: 93%

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Tozetto‐Mendoza¹,

Ibrahim²,

Tateno

et al. 2016

Medicine

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AIDS-associated Kaposi's sarcoma (AIDS-KS) caused by human herpes virus 8 (HHV-8) is the most severe and resistant form of KS tumor. Our aim was to verify whether there is an association between HHV-8 variability and development of AIDS-KS in Brazil by comparing the HHV-8 variability between individuals without and with KS. Saliva samples and blood, when available, were analyzed by polymerase chain reaction (PCR) techniques for detection of the fragments of ORF K1 of HHV-8, which were then genotyped and analyzed regarding the genetic variability. Our study described 106 positive cases for HHV-8 in the saliva from 751 AIDS patients without previous KS. In addition, we performed a phylogenetic analysis of HHV-8 in 34 of the 106 AIDS patients without KS and in 33 of the 37 patients with active KS. The distribution of HHV-8 genotypes A, B, C, and F in AIDS individuals was indistinguishable by comparing non-KS and KS groups, as well as regarding ethnicity. Considering the KS group, genotype B was associated with better prognosis of KS tumor. Interestingly, we found a particular profile of diversity within clade C and 2 recombinant patterns of HHV-8 in the saliva of AIDS individuals without KS. We emphasize the need to achieve standard genotyping protocol for ORF K1 amplification, thus allowing for substantial detection of HHV-8 variants. Our findings can shed light on the role of HHV-8 variability in the pathogenesis of AIDS-KS.

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Section: Discussionmentioning

confidence: 93%

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Tozetto‐Mendoza¹,

Ibrahim²,

Tateno

et al. 2016

Medicine

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“…It is becomingly increasingly apparent that DNA viruses may also exhibit significant intrahost diversity. For example, significant population variability has been observed in pseudorabies virus (61), HCMV (18), and Kaposi's sarcoma-associated herpesvirus (KSHV) (62), members of the alpha-, beta-, and gammaherpesviruses, respectively. Our results extend these findings and suggest that this is a general phenomenon of the Herpesviridae family, although the factors contributing to the diversity remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Epstein-Barr Virus Latent Membrane Protein 1 Genetic Variability in Peripheral Blood B Cells and Oropharyngeal Fluids

Renzette

Somasundaran

Brewster

et al. 2014

J Virol

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We report the diversity of latent membrane protein 1 (LMP1) gene founder sequences and the level of Epstein-Barr virus (EBV) genome variability over time and across anatomic compartments by using virus genomes amplified directly from oropharyngeal wash specimens and peripheral blood B cells during acute infection and convalescence. The intrahost nucleotide variability of the founder virus was 0.02% across the region sequences, and diversity increased significantly over time in the oropharyngeal compartment (P ‫؍‬ 0.004). The LMP1 region showing the greatest level of variability in both compartments, and over time, was concentrated within the functional carboxyl-terminal activating regions 2 and 3 (CTAR2 and CTAR3). Interestingly, a deletion in a proline-rich repeat region (amino acids 274 to 289) of EBV commonly reported in EBV sequenced from cancer specimens was not observed in acute infectious mononucleosis (AIM) patients. Taken together, these data highlight the diversity in circulating EBV genomes and its potential importance in disease pathogenesis and vaccine design. IMPORTANCEThis study is among the first to leverage an improved high-throughput deep-sequencing methodology to investigate directly from patient samples the degree of diversity in Epstein-Barr virus (EBV) populations and the extent to which viral genome diversity develops over time in the infected host. Significant variability of circulating EBV latent membrane protein 1 (LMP1) gene sequences was observed between cellular and oral wash samples, and this variability increased over time in oral wash samples. The significance of EBV genetic diversity in transmission and disease pathogenesis are discussed.

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“…Human herpes virus type 8 also known as Kaposi's sarcoma associated Herpes virus (KSHV) was first identified in 1994, in lesions of patients with Kaposi's sarcoma (KS) [1]. Human herpes virus type 8 (HHV-8) is a double stranded DNA virus and the most recently discovered human tumor virus [2].…”

Section: Introductionmentioning

confidence: 99%

Human Herpes Virus Type 8 among Female-Sex Workers

Nzivo¹

2017

JHVRV

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The prevalence of Human Herpes Virus type 8 (HHV-8) varies from one region to another and by populations. The modes of transmission and risk factors associated with HHV-8 among heterosexual adults are still not clear. HHV-8 has been associated with high risk sexual behavior. Female-sex workers (FSWs) are a high-risk group and therefore more vulnerable to sexually transmitted infections (STIs) compared to women in the general population. This review aimed at determining the global distribution of HHV-8 among FSWs and its associated risk factors. The secondary aim was to determine transmission and HHV-8 co-infection with Human Immunodeficiency Syndrome (HIV) among FSWs. We systematically searched literature in PubMed and Google Scholar. Twenty six studies among FSWs and women engaging in high risk sexual behavior from Africa, Europe, North and South America, Asia and the Mediterranean regions from 1996-2014 were reviewed. Results were presented in proportions. HHV-8 distribution among FSWs followed patterns of HHV-8 distribution in the general population in the world, that is, high among African and Mediterranean FSWs compared to other regions. The main risk factor associated with HHV-8 was African race. Sexual behavior associated with HHV-8 varied from one study to another. In conclusion, FSWs may not play a vital role in the transmission of HHV-8.

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Intrahost genetic variability of human herpes virus‐8

Cited by 10 publications

References 65 publications

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma

Epstein-Barr Virus Latent Membrane Protein 1 Genetic Variability in Peripheral Blood B Cells and Oropharyngeal Fluids

Human Herpes Virus Type 8 among Female-Sex Workers

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