Intramolecular electron conduction along DNA strands and their temperature dependency in a DNA-aligned cast film

Nakayama, H.; Ohno, Hiroyuki; Okahata, Yoshio

doi:10.1039/b107228e

Cited by 51 publications

(28 citation statements)

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“…Recently, Kasumov and co-workers [4] have pointed out the interplay between the conformation of DNA molecules on surfaces and their electrical properties, based on height measurements by AFM and by using pentylamine surface treatments. The relationship between the DNA structure and its electrical properties was also established by Nakayama et al [3], who studied the conductivity the DNA strands as a function of temperature, related to changes in the intramolecular stacking of base pairs close to the melting transition.…”

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confidence: 99%

“…The research on DNA conductivity is of interest as charge transfer seems to play a key-role in the protection of the genetic code [2]. Nevertheless, the experiments on the conductivity of DNA are far from having reached a consensus, with electrical behaviours ranging from insulator to metallic and even contact-induced superconductivity [2,3]. This variety of experimental results may certainly be related to the variety of available DNA deposition methods.…”

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confidence: 99%

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Localization and delocalization of charges injected in DNA

Heim

Mélin

Deresmes

et al. 2004

Applied Physics Letters

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The electrical properties of DNA molecules are investigated by charge injection and electric force microscopy experiments. Prior to injection, DNA molecules exhibit a weak positively charged state. We probe the electrical behaviour of DNA by measuring the localized or delocalized character of the DNA charge states upon injection of excess charges. We show that injected charges do not delocalize for overstretched DNA prepared by a receding meniscus technique, while the adjunction of spermidine during the deposition leads to relaxed DNA molecules exhibiting a charge delocalization over microns. The interplay between charge localization/delocalization and deposition techniques may explain that transport behaviors ranging from insulating to conductive have been reported for DNA deposited on surfaces.85.65.+h, 87.14.Gg, 87.64.Dz, 68.37.Ps

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Localization and delocalization of charges injected in DNA

Heim

Mélin

Deresmes

et al. 2004

Applied Physics Letters

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“…In this work, we present the temperature-dependent conductivity and structural evolution monitored through Raman spectroscopy measured on the DNA-lipid cast film between physiological and denaturation temperatures. These DNA-lipid films were previously studied by Nakayama et al [2], where disappearance of conductivity above the denaturation temperature was reported. We observed a substantial reduction in the DNA conductivity due to premelting effects starting at temperature as low as 40…”

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confidence: 99%

“…• C. Above this temperature, the double-stranded molecular conformation is destroyed, and consequently, the electrical conductivity is lost [2,3]. According to numerous theoretical models [4,5] even at physiological temperatures (∼40…”

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Effect of Premelting on Conductivity of DNA-Lipid Films

Kasumov

Nakamae

Cazayous

et al. 2009

Research Letters in Nanotechnology

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“…This is because one phosphate group per base pair can bond with one functional cation. 3 Herein, we describe the single molecular morphology of porphyrin/DNA complex nanowires consisting of a -stacked porphyrin assembly deposited on a solid surface. Atomic force microscopy (AFM) observations of the porphyrin/DNA complex reveal the characteristic structure of -stacking interactions between porphyrin molecules.…”

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Single Molecular Morphology of Porphyrin/DNA Complex

Takatoh¹,

Matsumoto²,

Kawai³

et al. 2005

Chemistry Letters

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A Porphyrin/DNA complex stabilized by both ionic bonds and stacking of conjugates is reported herein. Morphology observation on the single molecular level revealed that the complex exhibits rigid behavior and inhomogeneity with phase separation. These characteristics are the indication of the presence of stacking interaction between porphyrins along the DNA scaffold.One-dimensional molecular complexes are attracting much interest for the design of nanostructured materials with electronic and magnetic functions.1 Low-dimensional structures are usually available as the structural motifs in crystals, and accordingly have been a subject of solid-state physics and chemistry. Recently, the physics and chemistry of single molecules have emerged as a new field of material science. A method to isolate molecular complexes on the single molecular level and maintain the molecular structure on a solid surface would provide a new family of functional molecular wires. The porphyrin nanostructure has been investigated extensively because of the attractive photoelectric properties originating from its stacked -electron system, which extends to the molecular plane.2 In order to create a one-dimensional porphyrin architecture, ionic assembly of porphyrins with a DNA scaffold is advantageous to high-density integration. This is because one phosphate group per base pair can bond with one functional cation.3 Herein, we describe the single molecular morphology of porphyrin/DNA complex nanowires consisting of a -stacked porphyrin assembly deposited on a solid surface. Atomic force microscopy (AFM) observations of the porphyrin/DNA complex reveal the characteristic structure of -stacking interactions between porphyrin molecules.The porphyrin/DNA complex was constructed using, 5,10,15,20-tetrakis[4-trimethylammoniophenyl]porphyrin-tetratoluene-4-sulfonate (TMAP, purchased from STERM) ( Figure 1a), 4,5 and poly(dA)poly(dT) (50-mer, purchased from Amersharm Pharmacia Biotech). TMAP and DNA were dissolved in doubly distilled water at concentrations of 2 Â 10 À4 mol/L (M) and 20 units, respectively, and maintained for one day after stirring. A concentration of one unit is equal to 50 mg/mL of solution of DNA, which can be converted to 1:6 Â 10 À4 mol/L (M) for the number of bases (phosphate groups). Various ratios of the solution were mixed by stirring in order to examine the different compositions of the TMAP/DNA complex. The mixed solutions were then diluted 100 times with water, and 10 drops of the solution were placed onto freshly cleaved mica in order to deposit the complex. After 2 min of incubation, the mica surface was dried under nitrogen flow. The complex formation in the solution was confirmed by spectroscopic data obtained using a UV-vis absorption spectrometer (U-3300, Hitachi) and a CD spectrometer (J-720, Jasco). The morphology of the TMAP/DNA complexes was observed by AFM (Model Nanoscope IIIa Dimension 3000, Digital Instruments) operated in tapping mode with a silicon single crystal probe under ambient pressure. Figure 1B s...

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Intramolecular electron conduction along DNA strands and their temperature dependency in a DNA-aligned cast film

Abstract: Electroconductivity along a long DNA strand (ca. 10 microns length) in a DNA-aligned cast film of DNA-lipid complex was measured on a comb-type electrode (5 microns distance), and it could be reversibly regulated by temperatures across 70 degrees C.

Cited by 51 publications

References 12 publications

Localization and delocalization of charges injected in DNA

Localization and delocalization of charges injected in DNA

Effect of Premelting on Conductivity of DNA-Lipid Films

Single Molecular Morphology of Porphyrin/DNA Complex

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