Intramuscular Fat Selection in Rabbits Modifies the Fatty Acid Composition of Muscle and Liver Tissues

Zubiri-Gaitán, Agostina; Blasco, A.; Ccalta, Ruth; Satué, K.; Hernández, Pilar

doi:10.3390/ani12070893

Cited by 7 publications

(3 citation statements)

References 45 publications

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“…The glycolytic and energy metabolism was also affected by selection. No differences were found between the lines in the plasma glucose concentration neither in previous 22,2 > nor in this experiment. However, greater abundances of metabolites involved in the amino sugar metabolism (0.68 to 1.30 SD, P 0 ≥ 0.99), the fructose, mannose and galactose metabolism (0.54 to 1.77 SD, P 0 ≥ 0.97), and the pentose metabolism (0.53 SD, P 0 = 0.96) were found in the plasma of the L line.…”

Section: Other Metabolic Pathways Affected By Selectioncontrasting

confidence: 56%

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Divergent selection for IMF in rabbits: A story told by plasma metabolites

Zubiri-Gaitán

Blasco

Hernández

2023

Preprint

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This study provides a thorough comparison of the plasma metabolome of two rabbit lines divergently selected for intramuscular fat content (IMF). The divergent selection led to a correlated response in the overall adiposity, turning these lines into a valuable animal material to study also the genetics of obesity. Over 900 metabolites were detected, and the adjustment of multivariate models, both discriminant and linear, allowed to identify 322 with differential abundances between lines, which also adjusted linearly to the IMF content. The most affected pathways were those of lipids and amino acids, with differences between lines ranging from 0.23 to 6.04 standard deviations, revealing a limited capacity of the low-IMF line to obtain energy from lipids, and a greater branched-chain amino acids catabolism in the high-IMF line related to its increased IMF content. Additionally, changes in metabolites derived from microbial activity confirmed its relevant role in the lipid deposition.

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Section: Other Metabolic Pathways Affected By Selectioncontrasting

confidence: 56%

“…The differences in cholesterol and bile acids was also observed in the plasma of the L line on the 10th generation of selection 2 >. Additionally, previous experiments also found greater amount of triglycerides in the plasma of the L line 22,23 .…”

Section: Lipids Metabolismmentioning

confidence: 67%

Divergent selection for IMF in rabbits: A story told by plasma metabolites

Zubiri-Gaitán

Blasco

Hernández

2023

Preprint

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“…Each sire was mated with five dams, and, in order to minimize the inbreeding, only one male offspring of the sire from the highest-ranking mating was selected for breeding the next generation. Further information on the divergent selection experiment can be found in previous publications [15,16]. The present study was carried out with data from 1982 rabbits, 166 of which were from the base population, 874 from the High-IMF line (H) and 942 from the Low-IMF line (L).…”

Section: Animals and Phenotypesmentioning

confidence: 99%

Genome-Wide Association Study of Maternal Genetic Effects on Intramuscular Fat and Fatty Acid Composition in Rabbits

EL Nagar,

Heddi,

Sosa-Madrid

et al. 2023

Animals

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Maternal genetic effects (MGE) could affect meat quality traits such as intramuscular fat (IMF) and its fatty acid composition. However, it has been scarcely studied, especially in rabbits. The objectives of the present study were, first, to assess the importance of MGE on intramuscular fat and fatty acid composition by applying a Bayesian maternal animal model in two rabbit lines divergently selected for IMF. The second objective was to identify genomic regions and candidate genes of MGE that are associated with the traits of these offspring, using Bayesian methods in a Genome Wide Association Study (GWAS). Quantitative analyses were performed using data from 1982 rabbits, and 349 animals from the 9th generation and 76 dams of the 8th generation with 88,512 SNPs were used for the GWAS. The studied traits were IMF, saturated fatty acids (total SFA, C14:0; myristic acid, C16:0; palmitic acid and C18:0; stearic acid), monounsaturated fatty acids (total MUFA, C16:1n-7; palmitoleic acid and C18:1n-9; oleic acid), polyunsaturated fatty acids (total PUFA, C18:2n-6; linoleic acid, C18:3n-3; α-linolenic acid and C20:4n-6; arachidonic acid), MUFA/SFA and PUFA/SFA. The proportion of phenotypic variance explained by the maternal genetic effect ranged from 8 to 22% for IMF, depending on the model. For fatty acid composition, the proportion of phenotypic variance explained by maternal genetic effects varied from 10% (C18:0) to 46% (MUFA) in a model including both direct and additive maternal genetic effects, together with the common litter effect as a random variable. In particular, there were significant direct maternal genetic correlations for C16:0, C18:1n9, C18:2n6, SFA, MUFA, and PUFA with values ranging from −0.53 to −0.89. Relevant associated genomic regions were located on the rabbit chromosomes (OCU) OCU1, OCU5 and OCU19 containing some relevant candidates (TANC2, ACE, MAP3K3, TEX2, PRKCA, SH3GL2, CNTLN, RPGRIP1L and FTO) related to lipid metabolism, binding, and obesity. These regions explained about 1.2 to 13.9% of the total genomic variance of the traits studied. Our results showed an important maternal genetic effect on IMF and its fatty acid composition in rabbits and identified promising candidate genes associated with these traits.

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Plasma metabolomic profiling in two rabbit lines divergently selected for intramuscular fat content

Zubiri-Gaitán,

Blasco,

Hernández

2023

Commun Biol

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This study provides a thorough comparison of the plasma metabolome of two rabbit lines divergently selected for intramuscular fat content (IMF). The divergent selection led to a correlated response in the overall adiposity, turning these lines into a valuable animal material to study also the genetics of obesity. Over 900 metabolites were detected, and the adjustment of multivariate models, both discriminant and linear, allowed to identify 322 with differential abundances between lines, which also adjusted linearly to the IMF content. The most affected pathways were those of lipids and amino acids, with differences between lines ranging from 0.23 to 6.04 standard deviations, revealing a limited capacity of the low-IMF line to obtain energy from lipids, and a greater branched-chain amino acids catabolism in the high-IMF line related to its increased IMF content. Additionally, changes in metabolites derived from microbial activity supported its relevant role in the lipid deposition. Future research will focus on the analysis of the metabolomic profile of the cecum content, and on the integration of the several -omics datasets available for these lines, to help disentangle the host and microbiome biological mechanisms involved in the IMF deposition.

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Intramuscular Fat Selection in Rabbits Modifies the Fatty Acid Composition of Muscle and Liver Tissues

Cited by 7 publications

References 45 publications

Divergent selection for IMF in rabbits: A story told by plasma metabolites

Divergent selection for IMF in rabbits: A story told by plasma metabolites

Genome-Wide Association Study of Maternal Genetic Effects on Intramuscular Fat and Fatty Acid Composition in Rabbits

Plasma metabolomic profiling in two rabbit lines divergently selected for intramuscular fat content

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