Intramuscular Injection of Combined Calf Blood Compound (CFC) and Homeopathic Drug Tr14 Accelerates Muscle Regeneration In Vivo

Abstract

Skeletal muscle injuries in competitive sports cause lengthy absences of athletes from tournaments. This is of tremendous competitive and economic relevance for both the athletes and their respective clubs. Therapy for structural muscle lesions aims to promote regeneration and fast-track return-to-play. A common clinical treatment strategy for muscle injuries is the intramuscular injection of calf blood compound and the homeopathic drug, Tr14. Although the combination of these two agents was reported to reduce recovery time, the regulatory mechanism whereby this occurs remains unknown. In this in vivo study, we selected a rat model of mechanical muscle injury to investigate the effect of this combination therapy on muscle regeneration. Gene expression analysis and histological images revealed that this combined intramuscular injection for muscle lesions can enhance the expression of pro-myogenic genes and proteins and accelerate muscle regeneration. These findings are novel and depict the positive effects of calf blood compound and the homeopathic drug, Tr14, which are utilized in the field of Sports medicine.

<p>The Effect of Inflammation on the Healing Process of Acute Skin Wounds Under the Treatment of Wounds with Injections in Rats</p>

“…[24][25][26][27][28] Deproteinized Calf Blood Medicine (Actovegin ® preparation) is used in everyday clinical practice as it significantly affects regional blood flow and the intensity of local inflammation. [29][30][31][32][33] Collagen I type (Collost ® ) does not significantly affect inflammation but is effective as a wound healing stimulator. [34][35][36][37][38][39]…”

<p>Comparative Analysis of the Effectiveness of Some Biological Injected Wound Healing Stimulators and Criteria for Its Evaluation</p>

“…All selected injectables were of biological origin and derived from mammals; their effectiveness in wound treatment has been previously proven in clinical and experimental studies. These include collagen (Collost ® ; which contains native unreconstructed collagen obtained from the skin of cattle), [15][16][17][18][19] deproteinized calf blood hemoderivative (DCBH; Actovegin ® ), [20][21][22] and mesenchymal stem cells (SCs). [23][24][25][26][27][28] An obvious advantage of the injectable drugs is the ability to accurately administer a certain volume and dose of the drug to the same location in all animals, providing identical wound treatment conditions.…”