Intramuscular injection of skeletal muscle derived extracellular matrix mitigates denervation atrophy after sciatic nerve transection

Abstract

Peripheral nerve injury and the associated muscle atrophy has an estimated annual healthcare burden of $150 billion dollars in the United States. When considering the total annual health-related spending of $3.5 trillion, these pathologies alone occupy about 4.3%. The prevalence of these ailments is rooted, at least in part, in the lack of specific preventative therapies that can be administered to muscle while it remains in the denervated state. To address this, skeletal muscle-derived ECM (skECM) was injected directly in denervated muscle with postoperative analysis performed at 20 weeks, including gait analysis, force production, cytokine quantification, and histological analysis. skECM was shown to be superior against non-injected muscle controls showing no difference in contraction force to uninjured muscle at 20 weeks. Cytokines IL-1β, IL-18, and IFNγ appeared to mediate regeneration with statistical regression implicating these cytokines as strong predictors of muscle contraction, showing significant linear correlation.

Conducting polymer-based scaffolds for neuronal tissue engineering

“…Tissue engineering has the potential to overcome these limitations and restore function. [81][82][83][84] CPs have shown great potential in CNS tissue engineering because of their unique electrical and mechanical properties.…”

“…Peripheral nervous system (PNS) tissue engineering is a field of research focused on developing strategies to repair or regenerate damaged or injured peripheral nerves. 81,[122][123][124] The PNS consists of nerves outside the brain and spinal cord and has some degree of regenerative capacity. However, in the cases of severe injury or nerve damage, it needs tissue engineering approaches that can enhance and accelerate healing.…”

Magnetic fibrin nanofiber hydrogel delivering iron oxide magnetic nanoparticles promotes peripheral nerve regeneration

“…These injuries are frequently observed in patients who have experienced trauma [ 1 ]. Due to its elevated socioeconomic status, the United States annually allocates approximately $150 billion towards PNIs, a figure that directly corresponds to the considerable socioeconomic impact [ 2 , 3 ]. The treatment of peripheral nerve injury is usually based on the severity of the injury.…”

Injectable and Self-Healable Thermoresponsive Hybrid Hydrogel Constructed via Surface-Modified Graphene Oxide Loading Exosomes for Synergistic Promotion of Schwann Cells

“…Schwann cells (SCs), as glial cells, play a key role in the regeneration of axons. Orderly and organized migration of SCs facilitates extracellular matrix connections, greatly promoting axon regeneration by providing mechanical support and growth factors. − Therefore, promoting the growth, proliferation, and migration of SCs in peripheral neuropathy is of great significance for the treatment of peripheral neuropathy and the normal function of axons . The widely used treatment for DPN is still based on blood sugar control, combined with a variety of nerve growth factors to regulate the lesion site and promote the growth of SCs and recovery of nerve lesions .…”