Intranasal chitosan-DNA vaccines that protect across influenza virus subtypes

Sawaengsak, Chompoonuch; Mori, Yasuko; Yamanishi, Koichi; Srimanote, Potjanee; Chaicumpa, Wanpen; Mitrevej, Ampol; Sinchaipanid, Nuttanan

doi:10.1016/j.ijpharm.2014.07.005

Cited by 31 publications

(19 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synthetic polymers with cationic groups are widely used nowadays in medicine and biology as effective nonnatural immunostimulants [1,2] andasDNA-containing constructs for gene delivery. [3,4] They also demonstrate antimicrobiala nd antiviral activities.…”

Section: Introductionmentioning

confidence: 99%

“…[8][9][10][11][12][13][14] The above effects have been revealed and quantified for long cationic polymers with rather high degrees of polymerization (DP). [1][2][3][4][5][6][7][8][9][10][11][12][13][14] However,i nformation about the behavior of short polycations with lower DPs on the biological membranes is fragmented. For example, it has been reported that acationic polypeptide with al ower DP is able to laterally segregate anionic lipids from mixturesw ith neutral lipids,b ut loses this ability under physiological salt conditions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Influence of the Chain Length of Polycations on their Complexation with Anionic Liposomes

et al. 2015

View full text Add to dashboard Cite

A series of strong polycations is synthesized through the anionic polymerization of 2-vinylpyridine, followed by subsequent quaternization of the resulting polymer. Polycations based on quaternized 2-vinylpyridine (PVPQs) with degrees of polymerization (DP) from 20 to 440 are adsorbed on the surface of small anionic liposomes. Liposome/PVPQ complexes are characterized by using a number of physicochemical methods. All PVPQs are totally adsorbed onto the liposome surface up to a certain concentration at which saturation is reached (which is specific for each PVPQ). The integrity of the adsorbed liposomes remains intact. Short PVPQs interact with anionic lipids localized on the outer membrane leaflet, whereas long PVPQs extract anionic lipids from the inner to outer leaflet. Complexes tend to aggregate, and the largest aggregates are formed when the initial charge of the liposomes is fully neutralized by the charge of the PVPQ. PVPQs with intermediate DPs demonstrate behavioral features of both short and long PVPQs. These results are important for the interpretation of the biological effects of cationic polymers and the selection of cationic polymers for biomedical applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Influence of the Chain Length of Polycations on their Complexation with Anionic Liposomes

et al. 2015

View full text Add to dashboard Cite

show abstract

“…39 Chitosan has been widely used as a delivery platform for DNA vaccines for a variety of pathogens including Leptospirosis, 40 Coxsackievirus B3, 41 and influenza. 42,43 Chitosan nanoparticles have also been formulated with pDNA encoding HPV-16 E7, a tumor-specific antigen for immunotherapy against HPV-associated tumors. After intramuscular injection of the HPV-16 E7/chitosan nanoparticles, the vaccine platform induced CD8þ T-cell activation and proliferation, stimulated interferon (IFN)-g and interleukin (IL)-4 production, and reduced tumor size in a mouse model.…”

Section: Natural Polymersmentioning

confidence: 99%

Micro- and nanoparticulates for DNA vaccine delivery

Farris

Brown

Ramer‐Tait

et al. 2016

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

DNA vaccination has emerged as a promising alternative to traditional protein-based vaccines for the induction of protective immune responses. DNA vaccines offer several advantages over traditional vaccines, including increased stability, rapid and inexpensive production, and flexibility to produce vaccines for a wide variety of infectious diseases. However, the immunogenicity of DNA vaccines delivered as naked plasmid DNA is often weak due to degradation of the DNA by nucleases and inefficient delivery to immune cells. Therefore, biomaterial-based delivery systems based on micro-and nanoparticles that encapsulate plasmid DNA represent the most promising strategy for DNA vaccine delivery. Microparticulate delivery systems allow for passive targeting to antigen presenting cells through size exclusion and can allow for sustained presentation of DNA to cells through degradation and release of encapsulated vaccines. In contrast, nanoparticle encapsulation leads to increased internalization, overall greater transfection efficiency, and the ability to increase uptake across mucosal surfaces. Moreover, selection of the appropriate biomaterial can lead to increased immune stimulation and activation through triggering innate immune response receptors and target DNA to professional antigen presenting cells. Finally, the selection of materials with the appropriate properties to achieve efficient delivery through administration routes conducive to high patient compliance and capable of generating systemic and local (i.e. mucosal) immunity can lead to more effective humoral and cellular protective immune responses. In this review, we discuss the development of novel biomaterial-based delivery systems to enhance the delivery of DNA vaccines through various routes of administration and their implications for generating immune responses.

show abstract

“…Synthetic or subunit viral peptide vaccines stimulate the immune response in similar mechanism as split inactivated vaccines and may be formulated with pattern recognition receptor agonists or emulsions to enhance adjuvant activity. Plasmid DNA encoding an immunogenic viral protein can be electroporated [163], administered intranasally in conjugation with nanoparticles [164], or delivered with a biolistic system. Particle-mediated epidermal delivery of DNA encoding influenza HA was recently optimized in an experimental ferret model and, when administered to the abdomen or tongue, yielded HAI titers greater than 1:40 [165]; sublingual route of vaccine administration has potential to elicit mucosal immunity and mount protective secretory immunoglobulin A (sIgA) [166,167].…”

Section: Alternatives To Current Influenza Vaccine Strategiesmentioning

confidence: 99%

Steps toward a Universal Influenza Vaccine: Research Models and Comparison of Current Approaches

Wong¹,

Ross²

2016

Steps Forwards in Diagnosing and Controlling Influenza

View full text Add to dashboard Cite

The ability of influenza virus to adapt to various species and evade natural immunity makes the ubiquitous pathogen particularly difficult to eradicate. Annual reformulation of influenza vaccines is costly and time-consuming and has varying efficacy against influenza virus strains. Therefore, worldwide efforts aim to develop a universal influenza vaccine to prevent potential healthcare emergencies such as pandemic influenza threats, such as the 1918 Spanish Flu and pandemic Swine Flu of 2009. Efficacy of a universal influenza vaccine must overcome current challenges with subtype diversity, antigenic drift, and adequately protect against emerging reassortants from both environmental and agricultural sources. Furthermore, the manufacturing and production of vaccines largely influence the effectiveness of a vaccine and technological advancements may soon rival current vaccine strategies. This review discusses the evolution and diversity of influenza viruses, how viral glycoprotein hemagglutinin plays a dominant role in influenza surveillance and assessment of protection and compares the methodologies of current and upcoming vaccine options. While the obstacles remain daunting, growing knowledge of influenza evolution and immunity may lead to more viable candidates that protect against broader varieties of influenza viruses and help prevent future international health crises.

show abstract

Intranasal chitosan-DNA vaccines that protect across influenza virus subtypes

Cited by 31 publications

References 59 publications

The Influence of the Chain Length of Polycations on their Complexation with Anionic Liposomes

The Influence of the Chain Length of Polycations on their Complexation with Anionic Liposomes

Micro- and nanoparticulates for DNA vaccine delivery

Steps toward a Universal Influenza Vaccine: Research Models and Comparison of Current Approaches

Contact Info

Product

Resources

About