Intranuclear Coccidiosis in a Calf

Nishida, Tomoko; Hatama, Shinichi; Ishikawa, Yoshiharu; Kadota, Koichi

doi:10.1292/jvms.71.1109

Cited by 10 publications

(7 citation statements)

References 17 publications

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“…In a case of E. alabamensis infection, nuclear cleavage in epithelial cells was considered to be evidence that merozoites can escape from the host cell nuclei or cytoplasm without inducing their destruction. 14 In contrast, in the present cases, there was widespread desquamation of villus epithelial cells, which contained parasites. Moreover, epithelial cell nuclei with several parasites were disintegrated.…”

Section: Research-article2014contrasting

confidence: 76%

“…This possible process of pathogenesis resembles E. alabamensis infection. 14 The absence or atrophy of the thymus in cases 2 and 3 suggests that perhaps the calves were already immunocompro-mised, 19 and therefore susceptible to opportunistic infection. Thus, growth retardation in some congenitally compromised calves may be associated with this type of intranuclear coccidiosis.…”

Section: Research-article2014mentioning

confidence: 99%

“…8 Polymerase chain reaction amplification of the 18S ribosomal RNA coding region in the parasite genome followed by nucleotide sequence analyses were performed as described previously. 14 The primers used were Ei18Sf1 forward (5′-AGCTC GTAGTTGGATTTCTG-3′) and Ei18Sr1 reverse (5′-CTAGT CGGCATAGTTTATGG-3′). The nucleotide sequences obtained were compared to homologous sequences of previously characterized Cyclospora spp.…”

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confidence: 99%

“…Sections were also stained with phosphate buffered Giemsa solution (pH 6.4), and were differentiated and dehydrated with isopropyl alcohol. Many tissue sections from a previously published case of E. alabamensis infection 14 were also made and used as a control to compare parasite morphology.…”

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Intranuclear coccidiosis caused by Cyclospora spp. in calves

et al. 2014

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Abstract. Intranuclear coccidiosis in 3 calves is described. Two calves with no genetic relationship were kept in the same pen, and the third calf was on another farm. The animals suffered from watery diarrhea or severe emaciation. Histologic examination showed epithelial desquamation (cases 1 and 2) or atrophy (case 3) of the jejunal villi. Coccidial meronts or merozoites were present in the nuclei of the majority of villus epithelial cells. There were rare intranuclear macrogametocytes, macrogametes, microgametocytes, microgametes, and oocysts in cases 1 and 2, but these were more easily observed in case 3. Parasite 18S ribosomal RNA sequences from case 1 showed 99.5% sequence identity with Cyclospora sp. Guangzhou 1, which has been found in fecal samples of cattle from China. In addition to the molecular results in one of the cases, the fact that the microgametocytes and oocysts were noticeably smaller in size than those of Eimeria alabamensis in all cases indicates the close association between Cyclospora and the 3 cases described herein.

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Section: Research-article2014contrasting

confidence: 76%

Section: Research-article2014mentioning

confidence: 99%

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confidence: 99%

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Intranuclear coccidiosis caused by Cyclospora spp. in calves

et al. 2014

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“…Globule leukocytes were suggested to be of T cell or natural killer (NK) cell lineage in goats and cats [4,12,13]. Considering the existence of intraepithelial T lymphocytes with eosinophilic granules and their malignant counterparts including hypergranular γδ T cell lymphoma and NK-like T cell lymphoma in cattle [16][17][18], it is probable that granulated lymphocytes have been confused with globule leukocytes. In a feline NK-like T cell lymphoma [6], the neoplastic cells were characterized by nonmetachromatic cytoplasmic granules and epitheliotropism in the intestine and liver, and had no relation to mast cells.…”

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Mast Cell Sarcoma with Megakaryocytic Differentiation in a Calf

Fujimoto

Wada

Kanada³

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. A case of mast cell sarcoma in a 5-month-old Holstein female calf is described. Macroscopically, enlargement of the spleen, lymph nodes, tonsils and kidneys was noted, and there were tumor masses in the neck region and on the pleura and peritoneum. The pericardium and uterine and ureter walls were also involved by tumor. Most neoplastic cells had eosinophilic granules, which were metachromatic and positive for naphthol AS-D chloroacetate esterase and tryptase, whereas smaller numbers of cells were positive for factor VIII-related antigen, a marker of megakaryocytes. Some of the predominant type of these tumor cells were found within the epithelia of the lungs, tonsils, gastrointestinal tract, liver, ureters, urinary bladder and uterus. Their normal counterparts were considered to be globule leukocytes. KEY WORDS: cattle, epitheliotropism, globule leukocyte, mast cell sarcoma.doi: 10.1292/jvms.12-0236; J. Vet. Med. Sci. 74(12): 1643-1646, 2012 Mast cell tumors in dogs are the most frequent malignant or potentially malignant tumor of the skin [7]. In humans, systemic mastocytosis is a disease caused by a neoplastic proliferation of mast cells. Most cases have the skin lesions of urticaria pigmentosa, and the majority of these have a relatively benign course [2]. In contrast, cases without skin lesions have a worse prognosis and may progress to transformation into mast cell leukemia or, more commonly, acute myeloblastic leukemia [9]. Highly malignant mast cell disorders such as mast cell leukemia and mast cell sarcoma are cytologically distinct from indolent systemic mastocytosis [2], and are not always associated with the mastocytosis [3].In cattle, mastocytoma is a benign or low malignancy neoplasm of mast cell origin, occurring chiefly in the skin [8]. Although multiple skin lesions or metastases in visceral organs are not infrequent, cutaneous mastocytomas are characterized by rare or absent mitotic figures [8]. Leukemias of the mast cell lineage [19] or with both mastocytic and megakaryocytic differentiation [10] have been observed in cattle. Here, we report, in a calf, a mast cell sarcoma that possessed mast cell features such as metachromasia and tryptase positivity, but also factor VIII-related antigen expression in a minority of cells.A 5-month-old Holstein female calf exhibited enlargement of the superficial lymph nodes, emaciation, growth retardation, rough hair coat and a rectal temperature of 40.5°C. The next day, hematological values were as follows: hematocrit, 17%; white blood cell count, 8,560 cells/ µl with 2% neutrophils, 43% lymphocytes, 1% monocytes and 54% atypical cells with nuclear irregularity and/or coarse azurophilic granules in the cytoplasm. Antibodies to bovine leukosis virus (BLV) were not detected by the agar gel immunodiffusion test. A clinical diagnosis of calf-form leukosis was made, and euthanasia was performed seven days after the initial examination.At necropsy, the superficial lymph nodes were highly to moderately enlarged, and were grayish white and homogeneous on c...

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