Purpose To investigate the changes in choroidal thickness (CT), axial length (AL), and ocular perfusion pressure (OPP) accompanying intraocular pressure (IOP) reduction after trabeculectomy. Methods Thirty-nine eyes of 39 patients with primary open-angle glaucoma uncontrolled by medical therapy were included in this prospective and interventional study. All patients underwent a fornix-based trabeculectomy. The CT was measured by enhanced depth imaging-optical coherence tomography. IOP, AL, and systolic/ diastolic blood pressure were also measured, and OPP was calculated. All measurements were performed at baseline and 1 month after surgery. Results The mean IOP was 25.0 ± 5.8 mm Hg at baseline and 11.7 ± 2.6 mm Hg after trabeculectomy (Po0.001), and the mean subfoveal CT was 295±84 mm Hg at baseline and 331±82 mm Hg after trabeculectomy (Po0.001). The mean AL was 23.64 ± 0.98 mm at baseline and 23.54 ± 0.96 mm after trabeculectomy (Po0.001), whereas the mean OPP was 38.8 ± 6.2 mm Hg preoperatively, and 51.1 ± 7.3 mm Hg postoperatively (Po0.001). The change in CT negatively correlated with the change in IOP (r ¼ À 0.785, Po0.001) and AL (r ¼ À 0.693, Po0.001), whereas it positively correlated with the change in OPP (r ¼ 0.418, P ¼ 0.008). Conclusion These results suggest that the large IOP decrease following trabeculectomy causes choroidal thickening.In addition, CT changes are associated with IOP and AL reduction as well as OPP increase.