Intraocular tumour necrosis factor ligand related molecule 1 A links disease progression of proliferative diabetic retinopathy after primary vitrectomy

Li, Jianan; Xie, Ruotian; Wang, Jiaxing; Zhang, Wei; Jiang, Feng; Du, Mei; Wang, Xiaohong; Huang, Bo; Brant, Rodrigo; Zhang, Cheng; Yan, Hua

doi:10.1111/1440-1681.13284

Cited by 4 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TNF- α is a small molecule protein secreted by macrophages, and its expression is regulated by the NF- κ B signaling pathway. Wang et al showed that the levels of TNF- α in the tears of DR patients are proportional to the severity of the disease [ 31 ]. IL-1 β is mainly produced by macrophages, which promotes the release of neutrophils from bone marrow and induces monocytes to release lysosomal enzymes locally, resulting in local inflammation.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cell Exosomal miR-146a Mediates the Regulation of the TLR4/MyD88/NF-κB Signaling Pathway in Inflammation due to Diabetic Retinopathy

Zhang

Zhao

et al. 2022

Computational and Mathematical Methods in Medicine

View full text Add to dashboard Cite

Diabetic retinopathy (DR) is the main cause of vision loss in diabetic patients, which cannot be completely resolved by typical blood sugar control. Inflammation influences the development of DR, so reducing the inflammatory response in DR patients is crucial to the prevention of DR. Therefore, we explored the regulatory effect of bone marrow mesenchymal stem cell (BMSC) exosomes on inflammation in DR mice. In order to analyze the mechanism of action, we used BMSC exosomal miR-146a to treat microglias in DR mice to observe cellular changes and expression of inflammatory factors. It was found that BMSC exosomal miR-146a reduced the levels of proliferating cell antigen and B-cell lymphoma-2 in microglias of DR mice and increased Bcl-2-related X with cysteine aspartic protease-3. By analyzing the expression of inflammatory factors, we found that BMSC exosomal miR-146a reduced the levels of TNF-α, IL-1β, and IL-6, which suggested that miR-146a can alleviate inflammation in DR mice. Further exploration found that miR-146a reduced the activity of TLR4 and increased the activity of MyD88 and NF-κB. Furthermore, the overexpression of TLR4 reversed the effects of miR-146a on the proliferation, apoptosis, and inflammation of microglias. Our study demonstrated that BMSC exosomal miR-146a can regulate the inflammatory response of DR by mediating the TLR4/MyD88/NF-κB pathway, providing an experimental basis for the prevention and treatment of DR.

show abstract

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cell Exosomal miR-146a Mediates the Regulation of the TLR4/MyD88/NF-κB Signaling Pathway in Inflammation due to Diabetic Retinopathy

Zhang

Zhao

et al. 2022

Computational and Mathematical Methods in Medicine

View full text Add to dashboard Cite

show abstract

“…Many risk factors, such as duration of diabetes, poor metabolic control, and onset of diabetes before puberty [28] and levels of inflammatory cytokines after primary vitrectomy [29], have been proven to be related to the progression of DR. In our study, multivariate logistic regression analysis showed that the AH level of VEGF 165 b/ VEGF ratio was an independent negative factor in the progression of DR, which suggests that a lower VEGF 165 b/VEGF ratio may have predictive value in determining diabetic patients at risk for DR progression.…”

Section: Discussion/conclusionmentioning

confidence: 99%

Decreased Ratio of VEGF165b/VEGF in Aqueous Humor Predicts Progression of Diabetic Retinopathy

Jiang

Chong

et al. 2020

Ophthalmic Res

Self Cite

View full text Add to dashboard Cite

Background: Different splicing of vascular endothelial growth factor (VEGF) gene results in 2 families of VEGF, the proangiogenic isoforms (VEGF_xxxa) and the antiangiogenic isoforms (VEGF_xxxb). VEGF₁₆₅b is the major antiangiogenic isoform of VEGF and the most studied member of the VEGF_xxxb family so far. Objectives: To determine the concentration of VEGF₁₆₅b and VEGF in the aqueous humor (AH) in diabetic eyes with or without diabetic retinopathy (DR) and to address the predictive value of VEGF₁₆₅b/VEGF ratio for progression of DR. Methods: AH samples from 20 eyes in healthy controls (CON group), 40 eyes in diabetic patients without DR (nDR group), and 30 eyes in diabetic patients with mild nonproliferative DR (DR group) were collected. All of the patients were followed up for at least 5 years. VEGF₁₆₅b and VEGF levels of AH samples were measured by enzyme-linked immunosorbent assay (ELISA). The predictive value of the initial VEGF₁₆₅b/VEGF ratio for progression of DR was studied. Results: The mean concentration of VEGF₁₆₅b significantly decreased in diabetic eyes vs. controls. The mean concentration of VEGF significantly increased in the DR group vs. the CON group. The VEGF₁₆₅b/VEGF ratio was significantly lower in diabetic patients compared to the CON group. The VEGF₁₆₅b/VEGF ratio was significantly lower in diabetic patients compared to the control group. The mean follow-up was 66.1months (range 60–71 months). The risk of DR progression was greater with a lower VEGF₁₆₅b/VEGF ratio. Conclusion: The VEGF₁₆₅b/VEGF ratio is lower in the AH of DR patients and the decreased ratio of VEGF₁₆₅b/VEGF predicts DR progression.

show abstract

“…Diabetic retinopathy (DR) is one of the most destructive microvascular complications of diabetes mellitus. Intraocular neovascularization and diabetic macular edema (DME) are two major clinicopathologic features during the development and progression of DR [ 1 , 2 ]. The occurrence of neovascularization is closely related to the duration of diabetes mellitus.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Level of ANGPTL4 Correlates with the OCTA Metrics of Diabetic Macular Edema in NPDR

Gong

Qiao

et al. 2022

Journal of Diabetes Research

View full text Add to dashboard Cite

Purpose. To investigate the aqueous levels of angiogenic factors in nonproliferative diabetic retinopathy (NPDR) patients with diabetic macular edema (DME) and to ascertain their association with optical coherence tomography angiography (OCTA) metrics. Methods. This study enrolled 21 NPDR eyes with DME (NPDR/DME+), 17 NPDR eyes without DME (NPDR/DME-), and 16 diabetic eyes without retinopathy (DWR). Luminex bead-based multiplex array was used to measure the levels of 25 cytokines. OCTA system with a scan area of 3 × 3 mm was used to measure retinal thickness (RT), retinal volume (RV), superficial vessel density (SVD), deep vessel density (DVD), foveal avascular zone (FAZ) area, perimeter and acircularity index. Results. The levels of ANGPTL4 were significantly different among the three groups ( P < 0.05 ), in which NPDR/DME+ group had the highest level and NPDR/DME- group had a higher level than the DWR group (all, P < 0.0167 ). OCTA examination showed that, compared with DWR and NPDR/DME- group, RT and RV increased and the whole/parafoveal DVD decreased in NPDR/DME+ group (all, P < 0.05 ). Meanwhile, NPDR/DME- group had lower parafoveal DVD than the DWR group ( P < 0.05 ). Correlation analysis showed that the levels of ANGPTL4 were positively correlated with foveal/parafoveal RT and RV and negatively correlated with whole/parafoveal DVD in NPDR patients (all, P < 0.05 ). As the influencing factor of RT, RV, and DVD, every additional 103 pg/ml of ANGPTL4 was associated with an increase in foveal and parafoveal RT of 4.299 μm and 3.598 μm, respectively. Every additional 106 pg/ml of ANGPTL4 was associated with an increase in foveal and parafoveal RV of 3.371 mm3 and 17.705 mm3, respectively. Every additional 104 pg/ml of ANGPTL4 was associated with a decrease in whole and parafoveal DVD of 1.705% and 1.799%, respectively. Conclusions. The level of ANGPTL4 in aqueous humor of NPDR patients with DME was significantly increased and ANGPTL4 might predict RT, RV, and parafoveal DVD of DME in NPDR patients.

show abstract

Intraocular tumour necrosis factor ligand related molecule 1 A links disease progression of proliferative diabetic retinopathy after primary vitrectomy

Cited by 4 publications

References 43 publications

Mesenchymal Stem Cell Exosomal miR-146a Mediates the Regulation of the TLR4/MyD88/NF-κB Signaling Pathway in Inflammation due to Diabetic Retinopathy

Mesenchymal Stem Cell Exosomal miR-146a Mediates the Regulation of the TLR4/MyD88/NF-κB Signaling Pathway in Inflammation due to Diabetic Retinopathy

Decreased Ratio of VEGF165b/VEGF in Aqueous Humor Predicts Progression of Diabetic Retinopathy

Aqueous Level of ANGPTL4 Correlates with the OCTA Metrics of Diabetic Macular Edema in NPDR

Contact Info

Product

Resources

About