Intraoperative pleural lavage cytolopgy in lung cancer patients

Okumura, Meinoshin; Ohshima, S; Kotake, Yahiro; Morino, Hideo; Kikui, Masanori; Yasumitsu, Tsutomu

doi:10.1016/0003-4975(91)90318-k

Cited by 70 publications

(42 citation statements)

References 5 publications

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“…In Asian countries, the predominant histological type of esophageal carcinoma is squamous cell carcinoma, and tumors are frequently located in the thoracic esophagus. As in lung cancer (11)(12)(13)(14), the presence of exfoliated cancer cells in the pleural cavity after The results are presented as the means ± SEM. The isotonic NaCl buffer before cell suspension, n=5; the other solutions, n=3.…”

Section: Discussionmentioning

confidence: 99%

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Kosuga

Shiozaki

Ichikawa³

et al. 2011

Oncol Rep

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Abstract. This study aimed to investigate cytocidal effects of hypotonic shock on esophageal squamous cell carcinoma (ESCC) cell lines, and to apply pleural lavage with distilled water to surgery for ESCC. Three human ESCC cell lines, TE5, TE9 and KYSE170 were exposed to distilled water, and morphological changes in ESCC cells were closely observed under a differential interference contrast microscope connected to a high-speed digital video camera. Further, serial cell volume changes after hypotonic shock were measured using a high-resolution flow cytometer. To investigate the cytocidal effects of hypotonic shock on ESCC cells, re-incubation of ESCC cells was performed after hypotonic shock. Additionally, the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB), a Cl -channel blocker, during hypotonic shock were analyzed. Video recordings by high-speed digital camera demonstrated that hypotonic shock with distilled water induced cell swelling followed by cell rupture. Measurements of cell volume changes using a high-resolution flow cytometer indicated that severe hypotonicity with distilled water increased broken fragments of ESCC cells within 5 min. Re-incubation experiments demonstrated cytocidal effects of hypotonic shock on ESCC cells. Treatment of cells with NPPB increased cell volumes by the inhibition of regulatory volume decrease, which is observed during hypotonic shock, and enhanced cytocidal effects. These findings demonstrated the cytocidal effects of hypotonic shock on ESCC cells, and clearly support the efficacy of pleural lavage with distilled water during surgery for ESCC.

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Section: Discussionmentioning

confidence: 99%

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Kosuga

Shiozaki

Ichikawa³

et al. 2011

Oncol Rep

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“…Spread of malignancies to the pleural cavity, resulting in malignant pleural effusions and pleural metastatic disease are associated with a dismal prognosis, with most patients surviving only a few months after the diagnosis is made (40)(41)(42)(43)(44)(45)(46)(47). While NSCLC is the most common cause of malignant disease of the pleura, the pleural model used in this study is also relevant to other cancer types, including mesothelioma, breast, ovarian, and lymphoid cancers (48)(49)(50).…”

Section: Discussionmentioning

confidence: 99%

“…Such foci of disease are extremely difficult to diagnose preoperatively. Metastatic disease in the pleural cavity may indicate a more aggressive tumor biology an a higher risk of recurrence (12)(13)(14)(15)(16), which could contribute to the decreased survival often seen in these patients. As such, new techniques are needed to both diagnose and treat metastatic pleural spread of NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Minimally invasive localization of oncolytic herpes simplex viral therapy of metastatic pleural cancer

et al. 2005

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Purpose-Herpes simplex virus-one (HSV-1) oncolytic therapy and gene therapy are promising treatment modalities against cancer. NV1066, one such HSV-1 virus carries a marker gene for enhanced green fluorescent protein (EGFP). The purpose of this study was to determine whether NV1066 is cytotoxic to lung cancer and whether EGFP is a detectable marker of viral infection in vitro and in vivo. We further investigated whether EGFP expression in infected cells can be used to localize the virus and to identify small metastatic tumor foci (< 1 mm.) in vivo by means of minimally invasive endoscopic systems equipped with fluorescent filters.Experimental Design-In A549 human lung cancer cells, in vitro viral replication was determined by plaque assay, cell kill by LDH release assay, and EGFP expression by flow cytometry. In vivo, A549 cells were injected into the pleural cavity of athymic mice. Mice were treated with intrapleural injection of NV1066 or saline and examined for EGFP expression in tumor deposits using a stereomicroscope or a fluorescent thoracoscopic system. Results-NV1066 replicated in, expressed EGFP in infected cells and killed tumor cells in vitro.In vivo, treatment with intrapleural NV1066 decreased pleural disease burden, as measured by chest wall nodule counts and organ weights. EGFP was easily visualized in tumor deposits, including microscopic foci, by fluorescent thoracoscopy.Conclusions-NV1066 has significant oncolytic activity against a human NSCLC cell line and is effective in limiting the progression of metastatic disease in an in vivo orthotopic model. By incorporating fluorescent filters into endoscopic systems, a minimally-invasive means for diagnosing small metastatic pleural deposits and localization of viral therapy for thoracic malignancies may be developed using the EGFP marker gene inserted in oncolytic herpes simplex viruses.

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“…On the other hand, the first intraoperative pleural lavage study conducted in 1958 with 49 cases demonstrated for the first time that there could be tumor cells in pleural cavity in cases with no macroscopic pleural effusion or pleural invasion [1]. Many studies emphasized the significance of pleural lavage in planning lung cancer treatment [2][3][4][5][6][7]. In existing studies, positive pleural lavage cytology after thoracotomy have been calculated as 4-14% [2][3][4]6,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Many studies emphasized the significance of pleural lavage in planning lung cancer treatment [2][3][4][5][6][7]. In existing studies, positive pleural lavage cytology after thoracotomy have been calculated as 4-14% [2][3][4]6,8,9]. Regardless of pleural effusion, presence of tumor in pleural cavity indicates presence of more aggressive tumor.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Pleural Lavage Cytology Before and After Resection on Prognosis

Özkan¹

2017

Ann Clin Anal Med

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ÖzetAmaç: Plevral sıvı sitolojisinde tümör hücresi saptanması malignite açısın-dan ileri evre ve kötü prognoz göstergesidir. Çalışmamızda plevral sıvısı olmayan operabl malignite olgularında peroperatif plevral lavaj ile saptanabilecek tümör pozitifliğinin kitle ve mediastinal lenf nodu özelliklerine göre analizi amaçlanmıştır. Gereç ve Yöntem: Küçük hücrteli dışı akciğer kanseri nedeni ile opere edilen 199 olguda, diseksiyon öncesi ve rezeksiyon sonrası yapılan plevral lavajın sitolojik değerlendirme sonuçları cinsiyet, lezyon şekil ve boyutu, toraks bilgisayarlı tomografi ve Pozitron emisyon tomografisi (PET BT) lenf nodu tutulumu, lezyonun SUVmax değeri, lokalizasyonu, patolojik N1 ve N2 metastazı, lokal invazyon, tümörün histopatolojisi gibi değişkenler ile istatistiksel olarak karşılaştırıldı. Bulgular: Çalışmaya dahil edilen olgular 4 yıl takip edildi. On (%5) olguda tümör nüksü, 12 (%6) olguda uzak organ metastazı saptandı. Çok değişkenli analizde ilk lavaj sitolojisi ile uzak organ metastazı arasında ve son lavaj sitolojisi ile tümör nüksü arasında anlamlı iliş-ki saptanmıştır. Postoperatif uzak organ metastazının lenf nodu metastazı ve ilk lavaj sitoloji pozitifliği ile, tümör nüksünün PET BT lenf nodu tutulumu, lenf nodu metastazı ve son plevral lavaj sitoloji pozitifliği ile anlamlı düzeyde iliş-kisi saptanmıştır. Tartışma: Günümüzde plevral lavaj analizi ile ilgili çalışma-larda artışlar söz konusu olup lavaj zamanlaması ve örnek alımı ile ilgili standardizasyona ihtiyaç mevcuttur. Daha geniş seriler içeren çok merkezli çalış-malar sonucunda pozitif lavaj sitolojisinin evrelendirmede tıpkı malign efüz-yon gibi prognostik faktör olarak kabul edileceğini umuyoruz. Anahtar KelimelerAkciğer Kanseri; Plevral Lavaj; Sitoloji; Prognoz Abstract Aim: Regardless of pleural effusion, presence of tumor in pleural cavity indicates presence of more aggressive tumor. In this study, we analysed positive tumor results in preoperative pleural lavage in operable malignant cases with no pleural fluid according to the mass and mediastinal lymph node characteristics. Material and Method: Pleural lavage before preoperative dissection and after resection was performed on 199 cases that underwent surgery for non small cell lung cancer (NSCLC). Findings of lavage were statistically evaluated based on gender, lesion shape and size, lymph node involvement in positron emission tomography-computed tomography (PET-CT), SUV-max value of lesion, localisation of the lesion, N1 and N2 metastases, local invasion findings, histopathological type of tumor and type of resection. Results: Cases included in this study were followed for four years. Ten of the cases (5%) had tumor recurrence and 12 of them (6%) had distant organ metastasis. In multivariable analysis, significant correlation was found between the first positive pleural lavage cytology and postoperative distant organ metastasis; the last pleural lavage cytology and tumor recurrence; postoperative distant organ metastasis and lymph node metastasis and the first positive lavage c...

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Intraoperative pleural lavage cytolopgy in lung cancer patients

Cited by 70 publications

References 5 publications

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Minimally invasive localization of oncolytic herpes simplex viral therapy of metastatic pleural cancer

The Impact of Pleural Lavage Cytology Before and After Resection on Prognosis

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