Intraorale Diagnostik mittels konfokaler Laser-Scanning-Mikroskopie / Intraoral diagnostics using confocal laser scanning microscopy

Burmeister, M.; Schwanewede, H von; Stave, J.; Guthoff, Rudolf

doi:10.1515/bmt.2009.004

Cited by 6 publications

(1 citation statement)

References 19 publications

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“…The structure analysis of the biofilm and extracellular matrix exopolysaccharide was done by CLSM in the tissue of the upper airway and oral cavity (47,48). CLSM is superior to SEM because it does not require dehydration of the sample and consequently, the structure of a biofilm is better preserved.…”

Section: Quantification and Visualization Of Microbial Biofilmmentioning

confidence: 99%

Microbial biofilm in human health - an updated theoretical and practical insight

Licker

Moldovan

Hogea

et al. 2017

Revista Romana De Medicina De Laborator

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Section: Quantification and Visualization Of Microbial Biofilmmentioning

confidence: 99%

Microbial biofilm in human health - an updated theoretical and practical insight

Licker

Moldovan

Hogea

et al. 2017

Revista Romana De Medicina De Laborator

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In vivo imaging of enamel by reflectance confocal microscopy (RCM): non-invasive analysis of dental surface

2013

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The aim is to establish the feasibility to image in vivo microscopic dental surface by non-invasive, real-time, en face Reflectance Confocal Microscopy (RCM). Fifteen healthy volunteers referred at the Multidisciplinary Department of Medical-Surgical and Odontostomatological Specialties, Second University of Naples, Naples, Italy, were enrolled. A commercially available hand-held RCM (Vivascope(®)3000, Lucid, Rochester, NY, USA) was used to image in vivo the dental surface of the upper right and left central incisors of each volunteer. Totally, thirty vestibular surfaces of upper central incisors were imaged in vivo by RCM to preliminary image the dental surface and assess the feasibility of a more extended study on teeth. In vivo RCM was able to image the dental surface within the enamel, at a maximum depth imaging of 300 μm, with images good in quality and the capability to detect enamel structures such as enamel lamellae and enamel damages, such as unevenness and cracks. In conclusion, enamel "optical biopsy", gained by RCM imaging, revealed to be a non-invasive real-time tool valid to obtain architectural details of the dental surface with no need for extraction or processing the samples. RCM appears to be an optimum auxiliary device for investigating the architectural pattern of superficial enamel, therefore inviting further experiments aimed to define our knowledge about damages after etching treatments or bracket removal and the responsiveness to fluoride seals and the morphology of the tooth/restoration interface. Moreover, this device could also be used to detect relevant diseases like caries, or to assess surface properties to evaluate lesion activity.

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Non-invasive in vivo visualization of enamel defects by reflectance confocal microscopy (RCM)

et al. 2014

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The enamel defects (EDs) may present with a variety of clinical manifestations with increasing severity from the sole appearance of pale discoloration to remarkable structural alterations. EDs are responsible for higher caries receptivity. In vivo reflectance confocal microscopy (RCM) allows to image in vivo at microscopic resolution of the dental surface, thus avoiding the tooth extraction and the sample preparation because of its ability to optically scan living tissues along their depth. Aim of this study is the in vivo assessment at microscopic resolution of dental surfaces affected by EDs without resorting to invasive methods such as teeth extractions, to define histological findings occurring in chromatic and/or structural EDs. For the purpose, 15 children, referring at the Dental Clinic of the Second University of Naples, affected by several degrees of EDs, were enrolled and underwent in vivo RCM imaging to microscopically define the ED confocal features using a commercially available hand-held reflectance confocal microscope with neither injuries nor discomfort. Totally, 29 teeth were imaged. Results demonstrated images good in quality and the capability to detect EDs such as unevenness, grooves, and lack of mineralization according to their clinical degree of disarray. The present in vivo microscopic study on EDs allowed to highlight structural changes in dental enamel at microscopic resolution in real-time and in a non-invasive way, with no need for extraction or processing the samples. Further experiments could define the responsiveness to remineralizing procedures as therapeutic treatments.

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Intraorale Diagnostik mittels konfokaler Laser-Scanning-Mikroskopie / Intraoral diagnostics using confocal laser scanning microscopy

Cited by 6 publications

References 19 publications

Microbial biofilm in human health - an updated theoretical and practical insight

Microbial biofilm in human health - an updated theoretical and practical insight

In vivo imaging of enamel by reflectance confocal microscopy (RCM): non-invasive analysis of dental surface

Non-invasive in vivo visualization of enamel defects by reflectance confocal microscopy (RCM)

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