Intraperitoneal Administration of Oxygen/Ozone to Rats Reduces the Pancreatic Damage Induced by Streptozotocin

Abstract

Background: The rat model of streptozotocin (STZ)-induced pancreatic damage was used to examine whether a systemic oxygen/ozone mixture could be beneficial for the pancreas by reducing the machinery of the local detrimental mediators released by STZ. Results: The results showed that oxygen/ozone administration (150 µg/Kg i.p.) for ten days in STZ rats increased the endogenous glutathione-s-transferase (GST) enzyme and nuclear factor-erythroid 2-related factor 2 (Nrf2) into the pancreatic tissue, together with reduction of 4-hydroxynonenal (4-HNE) and PARP-1 compared to STZ rats receiving O2 only. Interestingly, these changes resulted in higher levels of serum insulin and leptin, and pancreatic glucagon immunostaining. Consequently, glucose metabolism improved as evidenced by the monitoring of glycemia throughout. Conclusions: This study provides evidence that systemic administration of oxygen/ozone reduces the machinery of detrimental mediators released by STZ into the pancreas with less local damage and better functionality.

Potential Cytoprotective Activity of Ozone Therapy in SARS-CoV-2/COVID-19

“…The increment in Nrf2 was in line with the significant reduction in 61 % of the level of the pro inflammatory cytokine IL-1β in ME-treated patients compared with basal levels, even IL-1β levels in ME remain 94 % higher than values in normal subjects. An imbalance between Nrf2/NF-κB has been proposed in other diseases like diabetic neuropathy [68] in which ozone experimentally demonstrated its efficacy equilibrating this disruption [62]. Similar trends were found in viral diseases, NF-κB pathways can support influenza A virus infection and promote pneumonia.…”

“…An imbalance between Nrf2/NF-κB has been proposed in other diseases like diabetic neuropathy [68] in which ozone experimentally demonstrated its efficacy equilibrating this disruption [62]. Similar trends were found in viral diseases, NF-κB pathways can support influenza A virus infection and promote pneumonia.…”

“…Moreover, low quantities of H 2 O 2 formed as consequence of O 2 /O 3 have a key role in the molecular mechanism. H 2 O 2 is crucial and a common activator of the modulation of NF-κB and Nrf2 pathways [60][61][62]. In addition, 4-HNE also sends a signal of transient oxidative stress and its effects depend on concentration as well as cell/tissue origin.…”

Modulation of Oxidative Stress by Ozone Therapy in the Prevention and Treatment of Chemotherapy-Induced Toxicity: Review and Prospects

“…In addition, H 2 O 2 can act as promotor of the Nrf2 pathway. The important role of Nrf2 induction by ozone in order to enhance the antioxidant systems has been described recently [31][32][33].…”

Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders

“…Based on this rationale, in this review we present wide scientific background indicating how a natural bioactive molecule with antioxidant property such as the ozone (O 3 ) can be indicated as a potential new strategy to delay neurodegeneration. This hypothesis is based on the widely demonstrated evidence regarding the interaction between O 3 and Nrf2 ( Galie et al, 2018 , Siniscalco et al, 2018 , Re et al, 2014 , Vaillant et al, 2013 ). We first describe the relevant, well known and documented molecular mechanisms related to antioxidant/anti-apoptotic/pro-autophagy processes targeted by the O 3 administration via Nrf2 biological pathway.…”