Intraperitoneal Injection as a Method of Psychotropic Drug Delivery in Adult Zebrafish

Thiopeptide Antibiotics Exhibit a Dual Mode of Action against Intracellular Pathogens by Affecting Both Host and Microbe

“…Overall, the treatments apparently reduced the bacterial burdens in the infected larvae, consistent with the finding that the survival duration of treated larvae was much longer than that of the untreated controls (Figures 2A and S1A). Furthermore, the anti-infective effect of TSRs was validated on an M. marinuminfected zebrafish adult system (Stewart et al, 2011) in which a one-time treatment, by intraperitoneal injection, with each compound at 5 mM significantly reduced the bacterial burdens and improved viability ( Figures 2B and S1B). Notably, the same therapeutic dose of isoniazid, the front-line anti-mycobacterial agent, had almost no effect upon M. marinum infection in either the larval or the adult model ( Figure 2).…”

Pharmacological study of the light/dark preference test in zebrafish (Danio rerio): Waterborne administration

“…As zebrafish are a relatively new animal model of behavioral pharmacology, the effective concentrations of various psychotropic compounds are unknown (Stewart et al, 2011a;Stewart et al, 2011c;Stewart et al, 2011d). Despite having the main neurotransmitters, peptides, and hormones found in mammals, there are differences between the physiology of the two species (Stewart et al, 2011c).…”

“…However, intraperitoneal injection causes stress in animals (Maximino et al, 2010b;Stewart et al, 2011a). Thus, alternative methods were sought to identify anxiolytic drugs in zebrafish.…”

Effective Method to Deliver Test Substance in Adult Zebrafish (Danio Rerio).

“…Intraperitoneal injection proves beneficial when drugs are insoluble in water or which needs substantially small amount of drug to be delivered to an individual animal. Stewart A. et al outlined a simple protocol for the intraperitoneal injection of drugs in adult zebrafish (Stewart et al, 2011). Intravenous injection of drugs to zebrafish has been a challenge because of the small vessel diameter.…”