Intraspecific Antagonism in Natural Populations of Wood-decaying Basidiomycetes

Rayner, A. D. M.; Todd, N.K.

doi:10.1099/00221287-103-1-85

Cited by 63 publications

(24 citation statements)

References 5 publications

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“…Minimum putative boundaries of the area occupied by each genotype were determined by enclosing points in which the same genotype was retrieved with a continuous line. Fungal individuals belonging to the same genotype were assumed to represent a unit of H. annosum populations and were defined as representatives of the same genet (38). We use the broader definition of genet by Anderson and Kohn (2) to include both homokaryotic and heterokaryotic genotypes.…”

Section: R Cmentioning

confidence: 99%

“…In this regard, H. annosum appears to represent an intermediate situation between root pathogens, with a great secondary spreadCorresponding author: M. Garbelotto; E-mail address: matteo@nature.berkeley.edu -ing ability (e.g., some Armillaria spp. and Phellinus weirii) (3,42), and wound pathogens or decay basidiomycetes (16,29,38), characterized by the presence of numerous less extensive genotypes in a limited space. Although mortality centers resembling those in pine and spruce stands can also be observed in Abies spp.…”

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confidence: 99%

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Genetic Structure of Heterobasidion annosum in White Fir Mortality Centers in California

Garbelotto¹,

Cobb²,

Bruns³

et al. 1999

Phytopathology®

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ABSTRACT.Garbelotto, M., Cobb, F. W., Bruns, T. D., Otrosina, W. J., Popenuck, T., and Slaughter, G. 1999. Genetic structure of in white fir mortality centers in California. Phytopathology 89546-554.The structure of Heterobasidion annosum populations was studied in 1.5 mixed-conifer sites in central and northern California. Study sites displayed mortality of white fir trees in enlarging discrete patches (mortality centers). At each site, fungal genotypes were defined by somatic compatibility tests. In two sites, further genetic and molecular analyses were performed on field genotypes and on homokaryons obtained by dedikaryotization of field heterokaryons. Isolates were found to be colonizing mostly the roots and the bole sapwood of white fir trees, and no significant infections of other tree species were observed. Each mortality center was characterized by the presence of several fungal genotypes, all belonging to the S intersterility group. Both homokaryotic and heterokaryotic strains were present in all sites. Multiple genotypes were retrieved in individual trees or stumps. Out of 228 fungal genotypes, 86% were found only within a single tree or stump, while 14% had spread to adjacent trees. The two largest genotypes had diameters of 9 and 10 m, and had colonized five and nine trees, stumps, or both, respectively. The maximum distance between two adjacent trees colonized by the same genotype was 6 m, and a highly significant correlation was found between tree diameter and distance of fungal "vegetative" spread. The largest clones were found in areas characterized by high tree and stump densities, and secondary spread of the fungus was more significant in denser stands. In most cases, original infection courts of existing genotypes could be traced to standing trees and not to stumps. The genetic analysis performed in two mortality centers revealed that most local genotypes had different mating alleles, and thus originated from unrelated basidiospores. In a few cases, the same mating allele was shared by two heterokaryons (n+n genome) or by a homokaryon (n genome) and a heterokaryon. Molecular analysis showed that nuclei bearing the same mating allele were identical, providing evidence that the two nuclei forming heterokaryons can act independently in the field and can be shared among isolates, presumably via di-mon mating or by separate matings of different portions of widespread homokaryons.

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Section: R Cmentioning

confidence: 99%

mentioning

confidence: 99%

Genetic Structure of Heterobasidion annosum in White Fir Mortality Centers in California

Garbelotto¹,

Cobb²,

Bruns³

et al. 1999

Phytopathology®

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“…The use in experiments of such large numbers of basidiospores could be expected to result in intense competition between developing individual genotypes, so that individuals are likely to be severely restricted (Rayner & Todd 1977, Rayner & Boddy 1988. As discussed by , the volume of wood ultimately occupied by H. annosum ©1997 Scandinavian University Press ISSN 0282-7581 Downloaded by [ECU Libraries] at 15:37 05 June 2016 might in consequence be smaller than in stumps inoculated with fewer spores.…”

Section: Introductionmentioning

confidence: 99%

Inoculum concentration and the colonization of picea sitchensis stumps by basidiospores of heterobasidion annosum

Redfern

Gregory

MacAskill

1997

Scandinavian Journal of Forest Research

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Inoculum concentration and the colonization of Picea sitchensis stumps by basidiospores of Heterobasidion annosum.The colonization of Sitka spruce stumps by Heterobasidion annosum, and the population structure of colonies, was determined 2-3 yrs after inoculation with a 10-fold dilution series of basidiospore suspensions. Artificially inoculated stumps were infected more frequently than the controls, which received only natural inoculum. The extent of colonization tended to increase with increasing dilution and reached a maximum at the penultimate dilution, but differences were not significant because of large within-treatment variation. At the higher dilutions stumps fell into poorly-colonized and heavily-colonized groups suggesting that some stumps are inhibitory to infection whereas others are extremely susceptible. Colonized areas resulting from the higher dilution treatments tended to comprise fewer larger genets than those from more highly concentrated spore treatments. The results suggest that the success of establishment by H. annosum in basidiospore-colonized stumps represents the outcome principally of intraspecific competition and the constraints on colonization operating within stumps.

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“…We have concentrated on the cytology of this process in the wood-decaying basidiomycete Coriolus versicolor, which shows a marked incompatibility reaction in plate culture (Rayner & Todd, 1977, 1979. Surprisingly, however, direct microscopical observation of fusing hyphae failed to reveal any differences in the cytological events which occurred between somatically compatible (self) and incompatible (non-self) anastomoses.…”

Section: Introductionmentioning

confidence: 99%

Cytology of Self Fusions in Hyphae of Phanerochaete velutina

Aylmore

Todd

1986

Microbiology

Self Cite

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Hyphal fusions forming in secondary mycelia of Phanerochaete velutina were examined using combined light and electron microscopy. Only 'self' fusions, occurring between hyphal compartments derived from the same colony, were studied. At points of contact, fusions formed from a single opening which expanded radially by highly localized lysis of the surrounding cell wall. Fusion pore enlargement failed to reach the full hyphal diameter and a rim of undissolved cell wall remained marking the original point of contact. Within 2 h of this, the compartments became re-partitioned by septum formation, synthesis being associated with mitotic division and beginning precisely at the site of fusion pore expansion. Septa formed at fusions showed identical structure and development to those occurring in unfused compartments.

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Intraspecific Antagonism in Natural Populations of Wood-decaying Basidiomycetes

Cited by 63 publications

References 5 publications

Genetic Structure of Heterobasidion annosum in White Fir Mortality Centers in California

Genetic Structure of Heterobasidion annosum in White Fir Mortality Centers in California

Inoculum concentration and the colonization of picea sitchensis stumps by basidiospores of heterobasidion annosum

Cytology of Self Fusions in Hyphae of Phanerochaete velutina

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