Intraspecific variation in isoenzyme patterns of phenotypically separated populations of Zostera marina L. in the south-western Netherlands

Heij, Hans de; Nienhuis, P.H.

doi:10.1016/0022-0981(92)90185-d

Cited by 29 publications

(14 citation statements)

References 27 publications

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“…Previous investigations examining isozyme polymorphisms revealed essentially no genetic diversity within populations and a low level of genetic distinction between geographically disjunct populations of eelgrass (14,15). These findings, in conjunction with the known vigorous rhizomatous growth of this and other seagrass species, have led to the notion (3, 15) that the wide distribution and general ecological success of seagrasses are based upon a vegetative growth strategy.…”

Section: Introductionmentioning

confidence: 73%

“…The poor knowledge of the minimal habitat requirements for seagrass growth, colonization and establishment mechanisms, genetic diversity, and reproductive modes requisite for the maintenance of ecologically successful populations hinders the development of sound management criteria (see ref. 13).Previous investigations examining isozyme polymorphisms revealed essentially no genetic diversity within populations and a low level of genetic distinction between geographically disjunct populations of eelgrass (14,15). These findings, in conjunction with the known vigorous rhizomatous growth of this and other seagrass species, have led to the notion (3, 15) that the wide distribution and general ecological success of seagrasses are based upon a vegetative growth strategy.…”

mentioning

confidence: 71%

“…Previous investigations examining isozyme polymorphisms revealed essentially no genetic diversity within populations and a low level of genetic distinction between geographically disjunct populations of eelgrass (14,15). These findings, in conjunction with the known vigorous rhizomatous growth of this and other seagrass species, have led to the notion (3,15) A video-image analysis system (Bio ma, Millipore) was used to determine relative migration distances of all restriction fragments and to estimate the length of fragments by using standard curves generated from HindiII-digested phage A DNA marker.…”

mentioning

confidence: 99%

“…These findings, in conjunction with the known vigorous rhizomatous growth of this and other seagrass species, have led to the notion (3,15) A video-image analysis system (Bio ma, Millipore) was used to determine relative migration distances of all restriction fragments and to estimate the length of fragments by using standard curves generated from HindiII-digested phage A DNA marker. The A DNA size fragments (23-2 kb) were visualized with ethidium bromide, and a ruler was used to measure migration distances.…”

mentioning

confidence: 99%

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Assessment of genetic diversity of seagrass populations using DNA fingerprinting: implications for population stability and management.

Alberte

Suba

Procaccini

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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Populations of the temperate seagrass, Zostera marina L. (eelgrass), often exist as discontinuous beds in estuaries, harbors, and bays where they can reproduce sexually or vegetatively through donal propagation. We examined the genetic structure of three geographically and morphologically distinct populations from central California (Elkhorn Slough, Tomales Bay, and Del Monte Beach), using multilocus restriction fragment length polymorphisms (DNA fingerprints Seagrasses form extensive meadows along the shores of all but the polar seas (1). Where they are found, these submerged marine angiosperms structure nearshore food webs and are highly productive (2,3). Seagrass systems worldwide serve as important and often critical habitats for a broad diversity of invertebrate and fish species, many of which are economically important (3, 4). In addition, seagrasses protect coastlines by minimizing erosion; increasing sedimentation, leading to enhanced recycling of nutrients; and improving water clarity (5).Zostera marina L., or eelgrass, is the dominant seagrass species in temperate waters and can achieve production rates exceeding 4 g of carbon m-2 day-l (2). Eelgrass reproduces both sexually and vegetatively and can colonize to depths of 30 m in clear waters but typically is restricted to shallow or intertidal depths in many estuaries (1-3, 6, 7). Although eelgrass is ecologically successful in very low-light environments (<100 ILmol of quanta m-2.s1l; refs. 6 and 7), the reduction in light penetration found in most industrialized coastal regions has severely restricted the depth distribution and abundance of eelgrass and other seagrass species (8-11).Losses worldwide of seagrass beds have accelerated at alarming rates in the last two decades because of physical disturbance (e.g., dredging, coastline development, fishing practices) and water quality deterioration most often realized as enhanced light attenuation by the water column because of particle loading, eutrophication, and nuisance algal blooms (8-11). Though some of the proximal causes for seagrass loss are increasingly evident (8)(9)(10)(11)(12), the importance of genetic diversity and gene flow for resource stability is unknown. The poor knowledge of the minimal habitat requirements for seagrass growth, colonization and establishment mechanisms, genetic diversity, and reproductive modes requisite for the maintenance of ecologically successful populations hinders the development of sound management criteria (see ref. 13).Previous investigations examining isozyme polymorphisms revealed essentially no genetic diversity within populations and a low level of genetic distinction between geographically disjunct populations of eelgrass (14,15). These findings, in conjunction with the known vigorous rhizomatous growth of this and other seagrass species, have led to the notion (3, 15) that the wide distribution and general ecological success of seagrasses are based upon a vegetative growth strategy. Consequently, a high degree of genetic similarity within pop...

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Section: Introductionmentioning

confidence: 73%

mentioning

confidence: 71%

“…Previous investigations examining isozyme polymorphisms revealed essentially no genetic diversity within populations and a low level of genetic distinction between geographically disjunct populations of eelgrass (14,15). These findings, in conjunction with the known vigorous rhizomatous growth of this and other seagrass species, have led to the notion (3,15) A video-image analysis system (Bio ma, Millipore) was used to determine relative migration distances of all restriction fragments and to estimate the length of fragments by using standard curves generated from HindiII-digested phage A DNA marker.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Assessment of genetic diversity of seagrass populations using DNA fingerprinting: implications for population stability and management.

Alberte

Suba

Procaccini

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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“…Information on clonal diversity in seagrass populations has been hampered in the past by low variability of traditional genetic markers such as allozymes (De Heij & Nienhuis 1992, Capiomont et al 1996, Waycott et al 1996, Schlueter & Guttman 1998. Recent reports using DNA-based genetic markers suggest that the clonal structure in seagrass beds varies within and among species.…”

mentioning

confidence: 99%

An ancient eelgrass clone in the Baltic

Reusch¹,

Boström²,

Stam³

et al. 1999

Mar. Ecol. Prog. Ser.

122

118

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We report that an eelgrass bed Zostera marina L. at the h a n d Islands, northern Baltic Sea, is dominated by a single genotype which extends over an area of approximately 160 X 40 m. In total, 47 individuals were sampled and genotyped for 6 highly polymorphic microsatellite loci. Forty-four of the ramets revealed the same 6-locus genotype, including 3 identical heterozygous loci. They were thus assigned to the same genet (= clone). To our knowledge, it represents the largest marine plant identified thus far. Based on estimates of horizontal rhizome growth rates, this clone may be more than 1000 yr old. The remarkable phenotypic plasiticity of a single genotype which dominates this site illustrates that there is no simple one-to-one relationship between genetic diversity and population persistence in changing and stressful environments.

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