Intrasplenic xenografting of human fetal pancreatic islet cell cultures into rats with experimental diabetes mellitus

Blyumkin, V. N.; Скалецкий, Н. Н.; Popov, Vsevolod L.; Shal'nev, B. I.; Danilov, M.A.

doi:10.1007/bf00834202

Cited by 2 publications

(4 citation statements)

References 2 publications

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“…The functional activity of the cultures in vivo was confirmed by their transplantation to animals with stable alloxan diabetes [49,72]. A manifest antidiabetic effect was observed in the case of introduction of cultured islet cells in the liver and spleen, as well as in the muscles [5,45,46,48,72]. Remission of experimental diabetes was noted in rat recipients before the completion of the experiment (20 weeks).…”

Section: Bulletin Ol Experimental Bfology and Medicine No 4 1994 mentioning

confidence: 85%

“…An important result of these studies has been the conclusion that there are no species-specific differences between the antigenic characteristics of cells of humans and animals belonging to different taxonomic groups. The successful experiments with xenotransplantation of cultured islet cells to animals with induced diabetes mellitus have substantiated the possibility of effective xenogeneic transplantation in clinical practice [5,42,44,[46][47][48]72,79]. It has been demonstrated that the use of precultured islet ceils makes it possible to attain a longterm remission of the diabetic state even without immunosuppression.…”

Section: Bulletin Ol Experimental Bfology and Medicine No 4 1994 mentioning

confidence: 99%

“…Remission of experimental diabetes was noted in rat recipients before the completion of the experiment (20 weeks). A long-term survival of previously cultured islet cells in the organism of a xenogeneic recipient was verified by histological examination of the site of transplantation: groups of transplanted cultured islet cells which had preserved their structure were discovered [5,48]. Successful experimental studies provided grounds for clinical transplantation of cultured islet cells of HFP.…”

Section: Bulletin Ol Experimental Bfology and Medicine No 4 1994 mentioning

confidence: 99%

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Transplantation of cultured fetal pancreatic islet cells in the treatment of insulin-dependent diabetes mellitus

Скалецкий¹,

Fateeva²,

Сухих³

et al. 1994

Bull Exp Biol Med

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Fetal tissue grafting has been used in Russia for some 15 years for the treatment of insulin-dependent diabetes meUitus with complications. The use of cultured islet cells from the fetal human, as well as animal, pancreas has been successful even in the absence of immunosuppression. This review concerns only studies carried out in the CIS and the rest of the former USSR. It describes the treatment of complications of diabetes mellitus (retinopathy, nephropathy, polyneuropathy), the treatment of diabetes mellitus in children and pregnant women, and the preparation of patients with diabetes mellitus for surgery. Key Words: transplantation of human and animal fetal tissues; treatment of insulin-dependent diabetes mellitus and diabetic retinopathy, nephropathy, and polyneuropathy; treatment of diabetes mellitus in children and pregnant women; preparation of patients with diabetes mellitus for surgeryThe failure of traditional methods of treatment of insulin-dependent diabetes mellitus (IDDM) called for new, nontraditional methods of treating this severe disease. Since the main cause of IDDM, which usually develops during childhood or youth (before the age of 35), is the total or near-total death of 13-cells of the pancreatic islets, the possibility of a pancreas transplant was explored. In the middle of the sixties, major advances occurred in transplantology and immunology, successful transplantation of the pancreas became a reality. To date, over 4000 clinical pancreas transplants have been performed all over the world [74]. Since transplantation of the pancreas is not a life-saving operation and the risk of adverse side effects and complications from the surgery per se, resulting from adjunct immunosuppressive treatment, is rather high, a transplant of the pancreas alone can hardly be justified in patients with IDDM. Such an operation is usually performed International Institute of Biological Medicine, Moscow at the terminal stage of diabetic nephropathy, when a kidney transplant is required to save the patient's life. According to data of the leading centers of transplantation, successful transplantation of the pancreas performed simultaneously with kidney grafting provides for stable normaliTation of glycemia and for the abolishment of insulin dependence during the first year postoperation in almost 70% of patients with IDDM, the one-year survival attaining 90% [75]. On the other hand, no marked positive effect of transplantation of the pancreas has been noted with respect to the course of diabetic angiopathies [80], although, admittedly, these angiopathies had been severe at the time of transplantation. This operation, which is costly and not without risk for the patient with IDDM, is inadvisable at the earlier stages of diabetic complications. Furthermore, transplantation of the pancreas is severely limited owing to the lack of aUogeneic donor organs.Recently, the surgical techniques of pancreas grafting have virtually reached the limit of their

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Section: Bulletin Ol Experimental Bfology and Medicine No 4 1994 mentioning

confidence: 85%

Section: Bulletin Ol Experimental Bfology and Medicine No 4 1994 mentioning

confidence: 99%

Section: Bulletin Ol Experimental Bfology and Medicine No 4 1994 mentioning

confidence: 99%

See 1 more Smart Citation

Transplantation of cultured fetal pancreatic islet cells in the treatment of insulin-dependent diabetes mellitus

Скалецкий¹,

Fateeva²,

Сухих³

et al. 1994

Bull Exp Biol Med

Self Cite

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“…Transplantation of allogeneic isolated islet cells allows one to avoide abdominal surgery. In 1983, human iL were transplanted to rats with experimentally induced diabetes [60]. The first allogenic iL transplantation into a Type I diabetic patient was reported in 1990 [61].…”

Section: Transplantation Of Donor Islets Of Langerhansmentioning

confidence: 99%

Stem Cells in the Treatment of InsulinDependent Diabetes Mellitus

et al. 2016

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Diabetes affects over 350 million people worldwide, with the figure projected to rise to nearly 500 million over the next 20 years, according to the World Health Organization. Insulin-dependent diabetes mellitus (type 1 diabetes) is an endocrine disorder caused by an autoimmune reaction that destroys insulin-producing -cells in the pancreas, which leads to insulin deficiency. Administration of exogenous insulin remains at the moment the treatment mainstay. This approach helps to regulate blood glucose levels and significantly increases the life expectancy of patients. However, type 1 diabetes is accompanied by long-term complications associated with the systemic nature of the disease and metabolic abnormalities having a profound impact on health. Of greater impact would be a therapeutic approach which would overcome these limitations by better control of blood glucose levels and prevention of acute and chronic complications. The current efforts in the field of regenerative medicine are aimed at finding such an approach. In this review, we discuss the time-honored technique of donor islets of Langerhans transplantation. We also focus on the use of pluripotent stem and committed cells and cellular reprogramming. The molecular mechanisms of pancreatic differentiation are highlighted. Much attention is devoted to the methods of grafts delivery and to the materials used during its creation.

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Intrasplenic xenografting of human fetal pancreatic islet cell cultures into rats with experimental diabetes mellitus

Cited by 2 publications

References 2 publications

Transplantation of cultured fetal pancreatic islet cells in the treatment of insulin-dependent diabetes mellitus

Transplantation of cultured fetal pancreatic islet cells in the treatment of insulin-dependent diabetes mellitus

Stem Cells in the Treatment of InsulinDependent Diabetes Mellitus

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