Intratumoral Photodynamic Therapy With Newly Synthesized Pheophorbide a in Murine Oral Cancer

Abstract

Photodynamic therapy (PDT) is a therapeutic alternative for malignant tumors that uses a photosensitizer. Our group recently synthesized photosensitizer pheophorbide a (Pa) from chlorophyll-a. The present study investigated the therapeutic effect of PDT using intratumoral administration of the synthetic photosensitizer Pa in an in vivo murine oral squamous cell carcinoma (OSCC) animal model. Pa accumulation was measured using the fluorescence spectrum and imaging in living C3H mice. Intratumoral treatment of Pa-PDT (IT Pa-PDT) significantly inhibited the growth of transplanted OSCC cells. Histopathological examination of tumor tissues showed that PCNA expression was significantly decreased, while TUNEL-stained cells were markedly increased in the IT Pa-PDT group compared to controls. IT Pa-PDT-induced apoptosis was confirmed by immunoblot. Reduction of Bcl-2 and cleavage of caspase 3 and PARP were observed in IT Pa-PDT. These data demonstrate that IT Pa-PDT inhibited tumor cell proliferation and induced apoptosis, which is correlated with the anticancer activity of IT Pa-PDT. These potent antitumor activities of IT Pa-PDT were observed in both the immunohistochemistry and Western blot experiments. Our findings suggest the intratumoral therapeutic potential of Pa-PDT on OSCC. Additionally, demonstrated detection of Pa using a fluorescence spectroscopy system or molecular imaging system provides a means for simultaneous diagnosis and treatment of OSCC.

Photodynamic Synergistic Effect of Pheophorbide a and Doxorubicin in Combined Treatment against Tumoral Cells

“…Our values are in excellent agreement with previous literature reports (Φ ∆ = 0.59 in EtOH; Φ ∆ < 0.02 in water) [ 62 , 63 ]. PhA has been evaluated as a PDT agent against different cancer malignancies [ 40 , 64 ]. In our study, PhA localizes in lysosomes ( Figure 8 ).…”

Nanobubble-embedded inorganic 808 nm excited upconversion nanocomposites for tumor multiple imaging and treatment

“…6c ), and selected fluorescein as a fluorescent marker. 35 After 10 min of 808 nm laser irradiation, the fluorescence of fluorescein isothiocyanate (FITC) was detected by LSCM at 450 nm, showing apoptosis. The FITC fluorescence intensity shown confirms that UCNP–CN@NBs with O 2 has a more significant signal than UCNP–CN@NBs without O 2 .…”

Morinda citrifolia: fatos e riscos sobre o uso do noni

“…A feoforbida atua de forma citostática seletiva, em comparação a células normais, em células de glioblastoma com IC50 na ordem de microgramas/ml (Cho et al, 2014). Ainda, a atividade antiproliferativa tem sido observada em outras linhagens celulares, como câncer de mama, melanoma, câncer de intestino (Baudelet et al, 2013) e mais recentemente em tumores de boca (Kim et al, 2016;Ahn et al, 2016).…”