Intratympanic manganese administration revealed sound intensity and frequency dependent functional activity in rat auditory pathway

Jin, Seong-Uk; Lee, Jaejun; Hong, Kwan Soo; Han, Mun; Park, Jang-Woo; Lee, Hui Joong; Lee, Sangheon; Lee, Kyu-yup; Shin, Kyung Min; Cho, Jin Ho; Cheong, Chaejoon; Chang, Yongmin

doi:10.1016/j.mri.2013.03.003

Cited by 10 publications

(14 citation statements)

References 19 publications

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“…9,11,16 Manganese-enhanced magnetic resonance imaging was used to trace the central auditory pathway in the living animals after either topical or systemic administration due to the fact that Mn ++ ions enter excitable cells through voltagegated calcium channels. [16][17][18] Manganese-enhanced magnetic resonance imaging has been used to follow neural activity in the brain, including regions of accumulated sound-evoked activity in normal animals, tinnitus-related brain regions, noise-induced activity increases in the brain, and the central neuroplasticity (including hyperactivity) and neurodegenerative mechanisms associated with age-related hearing loss. [19][20][21][22] Recently, alterations in calcium transport (possibly due to Ca ++ -ATPase dysfunction) in preclinical models of cardiac hypertrophy were visualized using MEMRI.…”

Section: Camentioning

confidence: 99%

Calcium Metabolism Profile in Rat Inner Ear Indicated by MRI After Tympanic Medial Wall Administration of Manganese Chloride

Zou

Pyykkö

2015

Ann Otol Rhinol Laryngol

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Objectives: To evaluate the efficacy of the novel method for the targeted delivery of Mn ++ to the inner ear and monitor calcium metabolism activity in the inner ear. Materials and Methods: Dynamic signal changes of Mn ++ in the rat inner ear were followed using T 1 -weighted magnetic resonance imaging (MRI) after administration of 2.5 µl MnCl 2 (500 mM) to the medial wall of the middle ear cavity. Results: Mn ++ passed through both the oval and round windows and distributed in the perilymphatic compartments, where it formed bright sharp lines along the fluid-cellular borders 12 minutes post administration and entered the endolymph sufficiently after 45 minutes. After 6 hours, the distribution of Mn ++ shifted from a fluid-dominant pattern to a cell-dominant pattern. Mn ++ concentrated in the area of the basilar membrane, periphery process, and soma of the spiral ganglion on day 2; became more distinguishable on day 4; declined on day 8; and remained detectable for 16 days post administration. Conclusions:The novel targeted delivery method efficiently introduced Mn ++ into the inner ear. The dynamic distribution pattern of Mn ++ in the inner ear shown by MRI indicates that this method can be used to monitor calcium metabolism activity in the inner ear.

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Section: Camentioning

confidence: 99%

Calcium Metabolism Profile in Rat Inner Ear Indicated by MRI After Tympanic Medial Wall Administration of Manganese Chloride

Zou

Pyykkö

2015

Ann Otol Rhinol Laryngol

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“…The mechanism underlying the analysis is that the Mn ++ acts as a Ca ++ agonist in addition to its T1 contrast effect. MEMRI was used to trace the central auditory pathway in the living animals after either topical or systemic administration because Mn ++ ions enter excitable cells through voltage-gated calcium channels [12][13][14]. Recently, alterations in calcium handling (possibly due to Ca ++ -ATPase dysfunction) in preclinical models of cardiac hypertrophy were visualized using MEMRI [15].…”

Section: Review Articlementioning

confidence: 99%

Targeted Delivery of Contrast Agents to the Tympanic Medial Wall at Minimum Amount and the Efficient Uptake in the Inner Ear through Oval and Round Windows

Zou¹

2015

Austin J Radiol

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Inner ear MRI after intratympanic administration of Gadolinium chelate (GdC) has become a valuable method in detecting endolymphatichy drops attributing to the efficient uploading in the inner ear while avoiding high dose intravenous application that can cause renal injury. In addition to the well-accepted round window pathway, the oval window was discovered as a dominant approach for the agents to move from the middle ear into the inner ear. In order to fine-tune this method, a targeted delivery of GdC to the medial wall of the middle ear cavity was developed in a minimally invasive way. The targeted tympanic medial wall administration was expended in the topical tympanic application of super paramagnetic iron oxide nanoparticles and manganese as contrast agents in the inner ear MRI. This review provides an overview of the developments in the field.

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“…Lee et al first reported that ME MRI using intratympanic administration provided a new and convenient tool to investigate the neuronal pathway between the cochlea and other auditory centers in the brain stem. Furthermore, their following study with intratympanic injection suggested that more firing of auditory neurons, or firing of many auditory neurons together for the pooled neural activity, was needed to achieve an effective response to a high sound intensity or frequency …”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, their following study with intratympanic injection suggested that more firing of auditory neurons, or firing of many auditory neurons together for the pooled neural activity, was needed to achieve an effective response to a high sound intensity or frequency. 13 To the best of our knowledge, there have been no systematic reports in the literature aimed at investigating the visualization of the auditory pathway through different local injection sites on ME MRI, which plays an important role in potential basic science research on the auditory pathway. In this current study, the dose-related neurotoxicity of manganese ions was addressed using rats as the subjects.…”

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confidence: 99%

Manganese‐enhanced MRI (ME MRI) in evaluation of the auditory pathway in an experimental rat model

Tang

Xiao

et al. 2016

NMR in Biomedicine

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This study aimed to explore the optimal dose and manner of administration for visualization of the auditory pathway on manganese-enhanced MRI (ME MRI). Twenty-four healthy male Sprague-Dawley rats were randomly divided into three experimental groups (n = 8 for Groups A, B and C). The rats in Groups A, B and C were subjected to MnCl injection through the tympanum, inner ear endolymph and perilymph, respectively (0.2 M for four rats and 0.4 M for the others in each group) and observed at 1, 2, 3, 4, 7 and 10 days after the operation with 3.0 T MRI. The signal intensity (SI) and dynamic changes of the auditory pathways at various times, and at two doses through three injection routes, were compared by statistical analysis. Administration of MnCl through the perilymph best showed the complete auditory pathway (P < 0.01), whereas administration though the tympanum only demonstrated part of the pathway. The SI was highest at 24 h after administration of the tracer and began to decline at 48 h. The SI of the auditory cortex was higher after the injection of 0.4 M MnCl than that of 0.2 M MnCl . ME MRI best demonstrated the whole auditory pathway at 24 h after the injection of 0.4 M MnCl through the perilymph in the rat, which provided an optimal method for the study of ME MRI of the auditory pathway in the animal model.

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Intratympanic manganese administration revealed sound intensity and frequency dependent functional activity in rat auditory pathway

Cited by 10 publications

References 19 publications

Calcium Metabolism Profile in Rat Inner Ear Indicated by MRI After Tympanic Medial Wall Administration of Manganese Chloride

Calcium Metabolism Profile in Rat Inner Ear Indicated by MRI After Tympanic Medial Wall Administration of Manganese Chloride

Targeted Delivery of Contrast Agents to the Tympanic Medial Wall at Minimum Amount and the Efficient Uptake in the Inner Ear through Oval and Round Windows

Manganese‐enhanced MRI (ME MRI) in evaluation of the auditory pathway in an experimental rat model

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