Intrauterine infection with human parvovirus B19: A light and electron microscopy study

Eo, Caul; Mj, Usher; Pa, Burton

doi:10.1002/jmv.1890240108

Cited by 66 publications

(31 citation statements)

References 24 publications

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“…Giant pronormoblasts are early erythroid cells with a diameter of 25 to 32 m, large eosinophilic nuclear inclusion bodies, and cytoplasmic vacuolization, and occasionally, "dogear" projections may be observed (as indicated in Fig. 4) (44,55,168,249). EM of cells reveals cytopathic ultrastructural changes that include pseudopod formation, marginated chromatin, and virus particles in the nucleus (378).…”

Section: Cytopathologymentioning

confidence: 99%

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Human Parvovirus B19

2002

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Parvovirus B19 (B19) was discovered in 1974 and is the only member of the family Parvoviridae known to be pathogenic in humans. Despite the inability to propagate the virus in cell cultures, much has been learned about the pathophysiology of this virus, including the identification of the cellular receptor (P antigen), and the control of the virus by the immune system. B19 is widespread, and manifestations of infection vary with the immunologic and hematologic status of the host. In healthy immunocompetent individuals B19 is the cause of erythema infectiosum and, particularly in adults, acute symmetric polyarthropathy. Due to the tropism of B19 to erythroid progenitor cells, infection in individuals with an underlying hemolytic disorder causes transient aplastic crisis. In the immunocompromised host persistent B19 infection is manifested as pure red cell aplasia and chronic anemia. Likewise, the immature immune response of the fetus may render it susceptible to infection, leading to fetal death in utero, hydrops fetalis, or development of congenital anemia. B19 has also been suggested as the causative agent in a variety of clinical syndromes, but given the common nature, causality is often difficult to infer. Diagnosis is primarily based on detection of specific antibodies by enzyme-linked immunosorbent assay or detection of viral DNA by dot blot hybridization or PCR. Treatment of persistent infection with immunoglobulin reduces the viral load and results in a marked resolution of anemia. Vaccine phase I trials show promising results

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Section: Cytopathologymentioning

confidence: 99%

“…Erythroblasts in the fetal liver exhibit signs of B19 infection, including pathognomic cytopathology, viral DNA, and antigen (3,55). In utero infection is persistent and characterized by severe anemia, high-output cardiac failure, and death (365).…”

Section: B19 Infection In Pregnancy (I) Hydrops Fetalismentioning

confidence: 99%

Human Parvovirus B19

2002

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“…Light microscopy has been used to detect virus inclusions in cells of several fetal organs [Burton, 19861. Thin section electron microscopy has shown parvovirus particles in the nucleus of erythroid precursor cells [Caul et al, 1988;Knisely et al, 19881 and also in cytoplasm and associated with cytoplasmic membranes (Field et al, in preparation). Using in situ hybridisation techniques, researchers found B19 DNA in fetal spleen, liver, kidney, heart, and lung [Anderson et al, 1988;Porter et al, 1988b;Salimans et al, 19891.…”

Section: Wiley-liss Incmentioning

confidence: 96%

Detection of B19 parvovirus in human fetal tissues by in situ hybridisation

Nascimento

Hallam

Mori

et al. 1991

Journal of Medical Virology

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Evidence of B19 parvovirus infection was sought by in situ hybridisation with biotinylated probes in 65 tissue samples from 32 pregnancies (fetuses, products of conception and/or placentas). Twenty-seven samples were reactive and the results were confirmed by other methods for B19 virus detection in 22 cases. The other methods used were in situ hybridisation with 3H and 35S labelled probes; dot-blot hybridisation with biotin and 32P labelled probes; polymerase chain reaction assay; negative stain and thin section electron microscopy; and radioimmunoassay for B19 antigen. The five false positive results by in situ hybridisation with biotinylated probes were considered to be due to non-specific biotin capture and were more frequent with unfixed samples than with formalin fixed material. It was concluded that while biotinylated probes offered advantages over radioactive probes for detecting B19 DNA by in situ hybridisation, positive findings should be confirmed by other methods.

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“…Geralmente é responsável por infecções na infância que causam eritema infeccioso (EI) 3 . Em alguns casos, pode estar associado a doenças severas, tais como: artropatias agudas ou persistentes, crises aplásticas transitórias (TAC), hodropsia fetal e perda fetal 4 , que podem ocorrer em infecções durante o segundo trimestre de gravidez 5 , miocardites 11 e ou hepatite.…”

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Presença de parvovírus B19 em Manaus, AM

Figueiredo

Lima

Almeida

et al. 2005

Rev. Soc. Bras. Med. Trop.

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RESUMO ABSTRACT Investigation of 1,107 cases of exanthematous diseases in Manaus allowed the identification of the first 47 cases of human parvovirus B19 in the city. Parvovirus B19 was characterized by a combination of signs and symptoms such as fever, chronic headache, arthralgia, myalgia and exanthema. The frequency of exanthema was higher in individuals lessO parvovírus humano B19, descrito por Cossart cols 6 em 1975, pertence à família Parvoviridae, é um patógeno humano comum, associado a inúmeras doenças. Geralmente é responsável por infecções na infância que causam eritema infeccioso (EI) 3 . Em alguns casos, pode estar associado a doenças severas, tais como: artropatias agudas ou persistentes, crises aplásticas transitórias (TAC), hodropsia fetal e perda fetal 4 , que podem ocorrer em infecções durante o segundo trimestre de gravidez 5 , miocardites 11 e ou hepatite.O parvovírus B19 humano já foi detectado em várias regiões do mundo, mostrando sua ampla ocorrência e distribuição universal 1 . No entanto, estudos sobre infecções causadas por este agente ainda são escassos no Brasil.As primeiras descrições do parvovírus no país datam de 1989, no Estado do Rio de Janeiro 7 , em doadores de sangue sadios e, em Belém 13 , em estudo realizado com crianças em fase aguda de doença exantemática.Sabe-se que vários agentes virais como rubéola, sarampo, parvovírus B19 e arboviroses (oropouche, mayaro e dengue) são responsáveis pela ocorrência de doenças exantemáticas e artropatias. Sendo assim, se fez necessário o diagnóstico diferencial das doenças exantemáticas, o que proporcionou a descrição dos primeiros casos de parvovírus B19 na Cidade de Manaus, Amazonas.

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Intrauterine infection with human parvovirus B19: A light and electron microscopy study

Cited by 66 publications

References 24 publications

Human Parvovirus B19

Human Parvovirus B19

Detection of B19 parvovirus in human fetal tissues by in situ hybridisation

Presença de parvovírus B19 em Manaus, AM

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